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Which of the following is a false statement regarding Polymerase Chain Reaction (PCR)?
A. PCR is a modified version of cellular replication that is used to amplify small amounts of DNA.
B. A single PCR cycle having of three steps: denature, anneal and elongate.
C. Every cycle results in a doubling of the number of DNA molecules within the sample.
D. A PCR reaction utilizes all of the enzymes that are used during cellular replication.
I dissolve 4µg of DNA in 1mL of water. How much of this solution would I need in order to add 10ng of DNA in a reaction?
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