Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Working Principle of Spectrophotometer?
The working principle of spectrophotometer is graphically presented in Figure.
Figure: Working principle of spectrophotometer
Spectrophotometer measures either the amount of light transmitted (% transmission) or the amount of light absorbed (A) by a sample, as you may have noticed from Figure. Here monochromatic light (beam of light at a single wavelength) is passed through a liquid sample. Depending upon the cells suspended in the culture, light is scattered. The scattering of light is directly proportional to the cell mass or indirectly to the cell number. More the number of cells, more would be scattering of light. The unscattered light is measured by a photoelectric cell and is recorded as 0% to 100% transmission (T).
However, in practice, cell density is represented in form of optical density or absorbance because itis directly proportional to the cell concentration while transmission is inversely related to cell density. Population growth can be easily measured spectrophotometrically as long as the population is high enough to give detectable turbidity. At very high concentration of cells, light scattered by one cell may be rescattered by another cell and it may appear as unscattered. This results in the loss of linearity between cell number and turbidity at very high cell concentration. Commonly used wavelengths for bacterial turbidity measurements are 540 nm (green), 600 (orange) or 660 nm (red). Estimation of cell number is based on turbidity measurement. Turbidity increases as the cell number increases.
#qcarrieruestion..
Excretory organ of agama lizard
Heat Exchangers : This is an integral part of cardio pulmonary bypass and is designed to cool and warm the perfusate. non-sterile water from ice bath or warm water passed through
a) Why are grasshopper and Drosophila said to show male heterogamity? Describe. b) Define female heterogamity with the help of an example.
In general compare and contrast the three functional classifications of joints according to movement. What are two characteristics that make synovial joints unique and different fr
Q. How are the male gametes and the male gametophytes formed in angiosperms? In the anthers of every stamen there are pollen sacs. Inside the pollen sacs there are microspore m
Observing root hairs Study the root hairs with a hand lens and view how they are constructed.
all things in this topic
Arteries - Circulation The arteries deliver blood from the heart. Figure shows the structure of arteries and the different layers of the vessel wall. The thick walls of these
Assuming independent assortment, what proportion of the offspring of the cross AaBbCcDd × AabbCCdd will have the aabbccdd genotype?
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd