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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
What is telophase? Telophase is the cell divides apart and screws itself.
Define Recommended Dietary Allowance for Folate (RDA)? Folate requirements are the intake levels necessary to prevent deficiency with clinical symptoms. The requirements are ex
What are the main functions of the blood? The blood is a means of substance transportation all by the body. The blood distributes nutrients, oxygen, antibodies, hormones, and c
Explain glycolysis? Name the two monosaccharides which readily enter the glycolytic pathway. Illustrate a diagrammatic sketch of the microscopic view of a mammalian sperm a
middle man of the body
explain digestion in protozoans
Determine the Basic Seven Food Groups - Nutritions? These basic seven food groups are: 1) Cereals and cereal products 2) Pulses (also meat and meat products) 3) Milk and m
Euthenics : It is the study of improvement of human races by providing better conditions. Euthenics means human improvement by altering external factors like as education or the co
Q. List the functions of insulin? a) Insulin controls the level of the sugar (glucose) in the blood. b) Insulin permits cells to use sugar (glucose) for energy. Cells cannot
What are hydrophobic molecules (or hydrophobic molecular regions)? What are hydrophilic molecules? How can they be characterized in relation to their polarity? Hydrophobic mol
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