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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Define Prevention of IDD - Iodized salt distribution? Many approaches to reduce iodine deficiency has been formulated. Some of these are reviewed herewith: Iodized salt dist
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What is mechanical digestion? In molluscs, arthropods, earthworms, birds and vertebrates, in general, which organs respectively participate in this type of digestion? Mechanica
We have end of semester exams coming up meaning that a majority of it will be over subatomic particles
If dimples may be considered a single-gene dominant trait, and 64% of the population features dimples, calculate the frequencies of heterozygotes and both types of homozygotes, ass
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Implementation The planned goals and strategies are translated into nursing intervention by means of the nursing care plan. It is essential that the client and family par
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