Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Define Urinary Excretion Test - Thiamin Status? The urinary excretion of thiamin is an index of recent dietary intake of the vitamin. Hence, determination of thiamin in urine s
Amino Acid - compound with an amino group and a carboxyl group attached to a central carbon Proteins make up 50% of dry mass of cells in our bodies E.g. of proteins are
1. You are observing adsorption of a virus to the host cell, it uses long leg-like structures, what are they called?
Q. What is ancylostomiasis? The Ancylostomiasis is a disease caused by the Necator americanus or Ancylostoma duodenale both hookworms belonging to the nematode phylum (roundwor
explain the cell survival curve
Ulcerative enteritis (quail disease) Ulcerative enteritis, caused by Clostridium colinum, is found in chicken, quails, pheasants, turkeys and some other birds. Clostridial or
Q. Explain Use of Toxic amino acids? Lathyrus sativus (kesari dhal) seeds contain toxic amino acid beta-oxalyl aminoalanine (BOAA), is considered to be responsible
Genome wide association studies must account for the fact that covering the entire genome with marker loci will produce ______ associations between linked marker alleles and possib
Q How does the amoeboid movement occur and what are examples of beings and cells that use such movements for locomotion? Amoeboid movements are created by cytoplasmic movements
Define Nutritional Support Management for chemotherapy? Antiemetics are used in the treatment of chemotherapy induced nausea and vomiting. Antiemetics become absolutely necessa
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd