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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Early human studies are normally conducted in healthy subjects to assess acute safety and, if applicable, to monitor biochemical and pharmacological responses. These studies also p
Individual Psychotherapy: It is conducted on one to one basis. It is effective when a therapeutic rapport develops between patient and therapist. The common types of indiv
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The excretory of lizard
Define sex
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Cost due to Damage to Equipment Cost due to Damage to Equipment, Property etc. Tooling may have to be redesigned, adding cost. Cost due to reduced output and delay
Infectious bursal disease (IBD) It is a highly contagious acute disease in young chicken up to 6 weeks of age causing high morbidity and low mortality. Bursa may atrophy at th
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