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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Define the Poverty and hunger - mutually causes, devastating effects? Measures of food deprivation, nutrition and poverty are strongly correlated. Countries with a high prevale
LETHAL GENE (2:1 Instead of 1 : 2 : 1 or 3 : 1) (i) All the genes present in an organism are not useful, but some of them show harmful effects and sometimes may cause d
what do our brain and heart do when we are asleep?
why green seeds reappeared in the f2 generation
Explain about the Spinal Trauma? Spinal trauma or spinal cord injury, commonly clue to accidents, fills, sports injury can result in serious disabling consequences. The spinal
The axons of all the baroreceptors in the body were destroyed at 2 AM. All else is normal. Which of the following statements is true for the system at 2:10 AM when compared to th
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In the ecological study of food interactions, what are the autotrophic beings called? In the Ecology autotrophic beings are called as producers because they synthesize the orga
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