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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Immunogenicity Based on immunogenicity studies and experience with other vaccines, the new meningococcal conjugate vaccine (Menactra) should be highly effective in preventing m
Fusion inhibitor After HIV binds to the host cell surface, a conformational change occurs in the transmembrane glycoprotein sub- unit (gp41) of the viral envelope, facilitatin
what are mendal low
Why is the human placenta referred to as haemochorial type? Name the hormone it secretes to facilitate parturition.
Antibacterial activity Rifaximin reaches high concentrations in the intestinal tract and is active in vitro against a broad range of enteropathogens, including ETEC, EAEC, Shi
What are taenias? What are the diseases caused by them? Taenias, also called as tapeworms, are platyhelminth animals (flatworms). The major diseases caused by taenias are taeni
A restriction enzyme or restriction endonuclease is an enzyme which cuts DNA at particular recognition nucleotide sequences with Type II restriction enzymes cutting double-stranded
Measles Adults born after 1956 who have not received 2 doses of live measles vaccine (not the killed vaccine that was commonly used in the 1960s) after their first birthday an
Define the effect of Deficiency of Vitamin C? Severe ascorbic acid deficiency results in the development of the disease known as scurvy, three important manifestations of scur
What are mineral salts? Where in living beings can mineral salts are found? Mineral salts are simple inorganic substances made of metallic chemical elements, such as iron, sodi
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