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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Explain the Digestive System ? The digestive system takes in food and processes it. Digestion is the mechanical and chemical breaking down of food into a form that can be used
What is plaque? Plaque is a film over the teeth and having of saliva, mucus, bacteria and the substances they produce. It may also have mineral salts of calcium and magnesium.
Types of overpopulation - Consumption overpopulation Consumption overpopulation is a phenomenon seen in technologically advanced and affluent countries such as U.S.A., Japan,
Q. Are the veins or the arteries constituted of more muscle tissue? How different are the walls of these two kinds of blood vessels? The arterial system has thicker muscle wall
Why is the dietary obtainment of iodine so important for thyroid functioning? The obtainment of iodine from the diet is significant for the thyroid because this chemical elemen
Q. What are the differences between anastral and astral mitosis? Astral mitosis is that in which there is formation of the aster a structure made by the centrioles. Anastral mi
Explain fixed performance system in oxygen therapy? Fixed Performance Systems: These devices are capable of delivering a fixed, preset concentration of oxygen regardless of t
Explain the Liver organ? The liver is the largest internal organ in the human body. It lies just below the diaphragm in the upper right area of the abdominal cavity. It is an i
Explain the Naturally occurring Food Chemicals? Pharmacologically active substances include vasoactive amines such as histamine, tyramine, tryptamine, phenylethylamine, and ser
Q. What is the evolutionary importance of the emergence of seeds in the plant kingdom? The evolutionary significance of the seed is related to the plant capability of distant c
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