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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Heterophagic intracellular digestion is the breaking into minute substances of external substances engulfed in the cell by pinocytosis or phagocytosis. Phagosomes or pinosomes fuse
Explain about the Probiotics? A mono or mixed culture of living organisms, which when ingested in certain amounts, has a positive impact on host health, beyond conventional nut
Illustrate the aqueous phase in sol processing In a sol possessing a continuous aqueous phase, the colloidal particles have an electrically charged surface. The ionized groups
HEARIN G - Sound waves struck ear drum, it starts vibrations, these vibrations are carried to MIS → f. ovalis → S. vestibule → Hellicotrema → S. Tympani → f. rotundus → mi
Long-Day Plants (LDP) - Plant Responses to Light-Dark Cycles The definition of this is exactly opposite to short-day plant. That is those plants which flower when given more t
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A complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline. The neuron is healthy; it has a stable resting voltage of -70 mill
Other Causes - Deforestation Natural enemies like termites, pests and several kinds of diseases affect the forests adversely. There is also massive destruction of forests beca
AGA R - AGAR It is cell wall mucopolysaccharide of some red algae (e.g. Gelidium, Gelidiella, Gracillaria). Agar is used as culture medium, thickener, stabilizer and as lax
keel is the characteristic feature of flower of 1.tomato 2. tulip 3.ubdugifera 4.aloe
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