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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Q. What are the major types of animal tissue? The major animal cell tissues are the epithelial tissue, the nervous tissue, the connective tissue and the muscle tissue.
The Features of Retrograde Peri-Implantitis include: - Periapical Bone loss without gingival inflammation. - Microflora consisting mainly of streptococci and nonmotile organ
EMERGENC E OF CONTEMPORARY BIOLOGY - 1 . Aristotle (384-322BC) - Greek Classified animal species and arranged them in hierarchies. Formulated 'Great chain of
Research methods used in studying a genetic disease change with technological advances. These changes can be seen in Reading 22.1: Discovering the Huntington Disease Gene, which de
What is the meaning of Body composition? It refers primarily to the distribution of muscle, fat, bone and other tissues in the body, and its measurement is often considered as
Explain the interaction of vitamin A with Iron? Iron: Iron status of an individual correlates with vitamin A. The deficiency of vitamin A has been found to be associated with
Q. What is the lymphatic system? The lymphatic system is a network of specialized valved vessels that drain interstitial fluid (lymph), the lymphatic system is as well responsi
Define Eosin - Methylene Blue (EMB) Agar (Levine) Peptone - 10.0 gm Lactose - 5.0 gm Dipotassium - 2.0 gm Hydrogen Phosphate Agar - 13.5 gm Eosin Y - 0.4 gm M
Define symptoms in Stomach and Mouth of pernicious anaemia? Stomach: The cells which secrete acid and enzymes are atrophied. The gastric secretions are devoid of acid, pepsin
Digestion of Carbohydrates Digestion of food starts in the mouth itself by the action of enzyme salivary amylase and the carbohydrates present in the food, particular
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