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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Nose Nose consists of external and internal parts. The inner lining of the nose contains millions of small receptors. These receptors are located on the sensory hairs.
DIGESTION Digestive enzymes break down food particles into smaller units. You will see that the final breakdown products of protein digestion are single amino acids or small c
Centrifugal Pump: This is available for clinical perfusion from 1976 (Bio Medicus Pump). It is disposable, causes less blood trauma and reduces the risk of massive air embolism.
What is Exposure Assessment Exposure Assessment : The qualitative and quantitative evaluation of the degree of intake likely to occur.
Explain about the Functional Properties of Proteins? It may be clear by now that functionality (as implied to food ingredients) refers to 'any property aside from the nutrition
Discuss about the Luria Nebraska procedure The Luria Nebraska procedure involves an age and education correction. It is accomplished by computation of a cutoff score for abnorm
If a color blind man marries a woman pure for normal color vision, it's probable that one of the below situations may result. Is it possible that a) All the children would be
Once you have identified a few relevant mRNA species, you wish to more closely examine their messages by sequencing. You do not have the time to search the genome and isolate the D
Radial cleavage - Metazoa Radial cleavage produces tiers or layers of cells one on top of another. Radial cleavage is also said to be indeterminate or regulative because each
Q. Can you explain Antibiotics? The antibiotics are the chemicals produced by microorganisms which are able to destroy or inhibit the growth of other microorganisms. The therap
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