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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
A mother has brought her 2-week-old infant to the emergency department due to the baby's persistent and increasing jaundice. Blood testing reveals that the infant's unconjugated bi
Define Classification of foods based on pH? Of these, the nature of the food, primarily the pH of food, is the most significant determinant of how severely the food will be pro
explain in detail various types of epithelial tissue
A laboratory mouse homozygous for an RFLP marker is mated to a wild mouse that is heterozygous for that marker. One of the heterozygous individuals resulting from this cross is mat
Q. Explain Microbiology of vegetables? Ans. All of us are well aware that vegetables are grown in soil, which, we already know, are a rnajor support system for the mi
A container is filled to a depth of 20.0 cm with water. On top of the water floats a 30.0-cm-thick layer of oil with specific gravity 0.700. What is the absolute pressure at the bo
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When the chromosomes are depleted of histones they are seem to have a central fibrous 'protein scaffold' or nuclear matrix to which the DNA is attached in loops. Therefo
Determine the Symptoms of Shigellosis Symptoms: Pathogenicity involves the release of lipopolysaccharide endotoxin, which infects the intestinal mucosa. Shigellosis ranges fro
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