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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
What are primary consumers? Can a food chain present quaternary consumers without having secondary or tertiary consumers? Can a tertiary consumer of one chain be a primary or secon
Reproductive health disorders to dairy livestock accounts to a loss of about Rs 30,000- 50,000 crore annually to the nation. Better herd management can minimize the occurrence of d
Chiasmata formation takes place where crossing over occurs. Here Chromatid segments are exchanged which contributes to genetic variability. The 46 homologous chromosomes arrange at
Q. What is Etiopathology? Who is likely to develop gout? What are the risk factors? Let us find out. Gout is caused when there is over production of uric acid in normal purine
Explain the Techniques of cardian examination? Examiner should use the fingertips or the part just proximal to them for palpation. The chest should be completely exposed and el
XYY humans are fertile males. XXX humans are fertile females. What do these observations reveal about the mechanisms of sex determination and dosage compensation?
Q. What are the three kinds of protein fibers of the connective tissue proper? The matrix of the connective tissue proper is made of elastic fibers, collagen fibers and reticul
It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates
Which is the brain region responsible for the regulation of breathing and blood pressure? The neural regulation of breathing, blood pressure and other physiological parameters
Key skills for healthy coping are: - Problem-solving and goal setting skills: Finding a problem and challenges, thinking of alternatives for dealing with them, testing those
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