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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
What is the fundamental difference between protozoans and algae? The basic difference among protozoans and algae is the fact that protozoans are heterotrophs whereas algae are p
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It is made up of three cusps, anterior, posterior and septal. The septal cusp is attached to the medial margin. The anterior cusp is attached to the superior-lateral part of the ma
What proportion of children with Down syndrome do you expect when women with down syndrome have children with men who have 46 chromosomes? Justify answer
Classification with its Justification Kingdom Animalia Animals, multi-cellular organisms with cells that lack a cell wall, many capable of movement or movement of some o
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Raw Material for Industry - impacts on biodiversity The industry, producing goods and services, relies and impacts on biodiversity directly. Much of the raw material that goes
a) How does the Hardy- Weinberg's expression (p²+2pq+q² =1) Describe that genetic equilibrium is maintained in a population? (b) List any two factors that can disturb the ge
Define Equilibrium Conditions in Multicomponent Systems? This chapter applies equilibrium theory to a variety of chemical systems of more than one component. Two different appr
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