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1. You are given a pair of primers that has been shown amplify a 125 bp DNA of interest specifically by a end point PCR- as there was only one PCR product with a size of 125 bp detected by agarose gel electrophoresis. How would you further develop this end-point PCR to be a quantitative real-time PCR? Please provide a rationale for each step that you choose to further develop the assay and what is the alternative if your first choice is not working.
2.You have been asked to develop a monoclonal antibody against the human His-tagged Casc4 recombinant protein that you purified from the expression construct that you designed earlier in the semester (assume the protein is soluble).
a.) Outline a brief protocol for generating this monoclonal antibody using mice as the host species.
b.) What is a method for purifying this antibody from culture supernatant?
3. Zach wants to analyze the expression of transferrin receptor (a surface transmembrane protein) in an epithelial cell line with and without treatment using a certain drug. a.) Outline a brief protocol for obtaining these results. b.) Why did you choose this method?
Differentiation is largely a matter of gene control and imprinting (CH3) plays a role, but ___ may also play a role.
what is sunandini
Group specificity Some enzymes prefer a specific functional group to be present\%on the substrate molecules. Example:- alcohol dehydrogenase acts on alcohols having -OH gr
All the water used in the home that goes into the drain or into sewage collection system is called waste water. Domestic sewage is the waste water from kitchens, bathrooms etc. It
Q. Generally how does a male animal realize that the female is receptive to copulation? In most vertebrate species with internal fecundation the females have reproductive cycle
Are sex-linked diseases associated only to genes of the X chromosome? There are so many X-linked diseases such like, hemophilia B, hemophilia A and adrenoleukodystrophy, but re
Why is quarrantine a must before introduction of a plant species from a dissimilar country? a) What is meant by apoplast pathway? Why does it occur in cortex and not in endoder
Phylum Phaeophyta (brown algae) 1) They are brown algae, apart from chlorophyll they have brown pigment i.e. fucoxanthin which predominates. 2) They are one of ,the most com
Why is it important that a cell membrane does not allow all dissolved substances to diffuse freely through it? If the cell membrane were freely permeable, harmful substances co
Chancroid Chancroid, caused by Haemophilus ducreyi, is rare in the US. A single dose of azithromycin or ceftriaxone is usually effective, but may be less effective in HIV-inf
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