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Metaphase
This is a period of short duration, the chromosomes are at the stage of their maximum condensation and , hence more clearly visible ,Upon each side of their centromeres, a plate or cup like dise ,formed of protein complexes and called kinetochore , appears in the depression of the chromatid of this side the kinetochore of each side is seen to be attached to a bundle of a number ( usually 20 to 40 ) of newly formed spindle fibres called discontinuous , or attachment , or chromosomal , or interzonal spindle fibres . The opposite ends of these fibres are attached to the spindle poles of their respective sides. These fibres pull the scattered chromosomes and align then in a fixed specific manner at the equatorial plane of the spindle forming what is known as the equatorial or metaphase plate.
Carbon dioxide needed for photosynthesis enters the leaf via the stomata but water is also lost when these pores are open. Water is often limiting and to limit water loss through s
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