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Metaphase
This is a period of short duration, the chromosomes are at the stage of their maximum condensation and , hence more clearly visible ,Upon each side of their centromeres, a plate or cup like dise ,formed of protein complexes and called kinetochore , appears in the depression of the chromatid of this side the kinetochore of each side is seen to be attached to a bundle of a number ( usually 20 to 40 ) of newly formed spindle fibres called discontinuous , or attachment , or chromosomal , or interzonal spindle fibres . The opposite ends of these fibres are attached to the spindle poles of their respective sides. These fibres pull the scattered chromosomes and align then in a fixed specific manner at the equatorial plane of the spindle forming what is known as the equatorial or metaphase plate.
Tongue grafting Top of the stock is cut diagonally and in an upward direction. Scion is cut diagonally in downward direction. A second cut is given from above downwards,
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