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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Explain Ventricular Fibrillation (VF) and Pulseless Ventricular Tachycardia (VT) The commonest rhythm seen in cardiac arrest is VF, which may be preceded by a short period of
If the generation time (t) is the incubation time (t) per generation (G), or t = t/G, rewrite the formula you derived in question 2 for bacterial population growth in terms of incu
Autumn (fall) overturn In autumn or early winter the air temperature falls, resulting in the cooling of the surface waters. When the surface water cools to 4°C it becomes dens
Vitamin B 6 (Pyridoxine hydrochloride) Pyridoxine hydrochloride is a white, crystalline powder, practically odourless. The dry substance is sufficiently stable in air. With pr
Types of cells in Phloem The phloem consists of 4 types of cells: i).sieve elements, ii) companion cells, iii) phloem parenchyma and sometimes iv) phloem fibres. The metabolit
What are the main human diseases caused by prions? The main called human diseases of such type are the Creutzfeldt-Jacob disease (CJD), the kuru and the Gerstmann-Sträussle-Sch
Explain Direct Microscopic Count - Measurement of Microbes? The most obvious way to determine microbial number is through direct counting under a microscope. In this method, a
Explain the Most Probable Number (MPN)? MPN is a statistical estimating technique which is based on the fact that more the number of bacteria in a sample, more is the dilution
General necessities for surgery The actual surgical technique of dental implant placement, it would be appropriate to recapitulate the basic principles of surgery. The two prin
Explain Congenital Heart Disease (CHD)? Congenital heart disease is an abnormality at birth in cardiac circulatory structure or function. Optimal management of CHD aims not mer
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