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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
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Grazing Food Chain - Food Chain Grazing food chains are quite familiar to most of us. Cow or deer grazing in a field represent a grazing food chain. Similarly, eating of phyto
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what is the function of dna primer
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A solid compound was found to contain 42.11% C, 51.46%O and 6.43% H by weight. The molecular weight of the compound was 341. What is the molecular formula of the compound?
ENZYME INHIBITION Enzymes are often inhibited by the presence of suitable inhibitors. Much of current drug therapy is based on this. Basically, there are three major cla
Define Basic Terminology in Relation to Nutritional Requirements? By now you would have a good understanding that nutrient requirements are affected by a number of factors and
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Define Iron Response Element and Iron Regulatory Protein? The Iron Response Element (IRE) and the Iron Regulatory Protein (IRP) play key roles in co-ordinating regulation of ir
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