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Your methods so far have given some success! You now want to look at many different tissues in several individuals, some of whom have the mutation, some who are controls. How can you efficiently generate and amplify cDNA from each individual and tissue that can be used for directional cloning? Remember, you want to do many samples from many tissues, so starting with total cellular RNA fractions containing mRNA is where you need to put your effort and resources!
Structure and Content of NEUROPSYCHOLOGICAL BATTERY The battery contains 269 items, each of which may be scored on a 2- or 3- point scale. A score of 0 indicates normal perform
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The PCR (polymerase chain reaction) is an extremely simple as immensely powerful methods. It permits enormous amplification of any particular sequence of DNA
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what is the Symptoms of VIBRIO PARAHAEMOLYTICUS Symptoms: A total of greater than one million organisms may cause disease. Symptoms of intoxication which range from mild to se
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Each of the three NADH molecules formed per turn of the cycle yields 3 ATPs and the one FADH2 yields 2 ATPs by oxidative phosphorylation (whereas some measurements indicate in whic
Q. In which part of the digestive tube is water is chiefly absorbed? What about the mineral ions and vitamins? Most part of water, mineral and vitamins ions are absorbed by the
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