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What does the cell use NAD+ and NADP+ for? What is the difference between the two? In what types of biological pathways is NAD usually used in? NADP?
Explain what DNA-bar coding is and how it is used to study the epidemiology of viral diseases caused by insect vector-virus complexes; what can be learned using this approach?
Explain how the microtubule binding activity of dynein, dynactin, and kinesin II is regulated by specific kinases and phosphatases during organelle transport.
Suppose you are purifying a protein on a weak cation exchanger (pH 7.5) and it comes off at 300mM salt. predict what salt concentration (lower, same, higher) the protein would come off if you used a strong cation exchanger.
Smilin is a protein that causes people to be happy. It is inactive in many chronically unhappy people. The mRNA isolated from a number of different unhappy persons in the same family was found to lack an internal stretch.
How may you use site-directed mutagenesis to determine whether this residue is essential for proton conduction?
Different types of normal tissues in the human body have different mitotic indices. From the following list, which normal tissues would you expect to have the highest mitotic index: muscle, skin, kidney, or lung? Explain your answer.
What must the intracellular 3-phosphoglycerate/1, 3-diphosphoglycerate ratio be to make the phosphoglycerate kinase reaction thermodynamically favourable in the direction of 1, 3-diphosphoglycerate synthesis.
Describe how a Single Nuclear Polymorphism in each INTRON, EXON, and PROMOTER would negatively impact the function/product of the gene.
What is the best explanation for why tracheary elements are absent in the center of many monocot roots, but present in other seed plant roots?
How various molecules of ATP are USED UP in the glycolysis pathway.
Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase.
Describe the mechanisms through which input from GABAergic inhibitory neurons reduce action potential firing and neurotransmitter release.
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