Predict salt concentration the protein would come off

Assignment Help Biology
Reference no: EM1397736

Suppose you are purifying a protein on a weak cation exchanger (pH 7.5) and it comes off at 300mM salt. predict what salt concentration (lower, same, higher) the protein would come off if you used a strong cation exchanger. explain the logic behind your prediction in terms of the differences between a weak and strong exchanger.

Reference no: EM1397736

Questions Cloud

What information about brainless would be gained : Reporter genes are often used to study how the transcription of a particular gene is controlled during fruit fly development. You are interested in a gene called brainless that you think is involved in neural cell development.
Probability of random sample-sample mean sat score : What is the probability a random sample of 75 students will provide a sample mean Sat score within 20 of the population mean?
Advantageous from the organism perspective : The cells that will eventually develop into gamete-producing tissue are typically sequestered away from the developing organism quite early in development.
Conduct a general swot analysis for the sabic company : Conduct a general SWOT analysis for the SABIC Company, showing your methodology and conduct SWOT analysis for the SABIC Company, focusing more on the issues related to MIS and POM, showing your methodology.
Predict salt concentration the protein would come off : Suppose you are purifying a protein on a weak cation exchanger (pH 7.5) and it comes off at 300mM salt. predict what salt concentration (lower, same, higher) the protein would come off if you used a strong cation exchanger.
Compute the standard error of the mean : Compute the standard error of the mean O(funny o with line) line over x, for sample sizes of 50, 100, 15o, and 200. What can you say about the size of the standard error of the mean as the sample size is increased?
What is the net charge of this peptide at ph : Consider the following peptide: Asp-Lys-Tyr-Glu-His-Cys-Glu-Lys-Ala-Arg-Cys.
Standard error of the mean as the sample size : Compute the standard error of the mean O(funny o with line) line over x, for sample sizes of 50, 100, 15o, and 200. What can you say about the size of the standard error of the mean as the sample size is increased?
What are some of the sites of coronary occlusion : How can you explain the fact that a patient who has cardiac ischemia as indicated by angina pectoris, and who survives a cardiac infarction, may be relieved of his pain afterwards?

Reviews

Write a Review

Biology Questions & Answers

  Find the name of large cartilage of anterior larynx

The surface area of lungs in humans is about seventy square meters. Discuss how can this be if the lungs are located in the small space of the and Find name of the large cartilage of the anterior larynx?

  Expalining the axial musculature:

Which of following are true of axial musculature:It arises elsewhere, however inserts on the axial skeleton. It makes up 40 percent of the body musculature.

  Compute the recipe to make desired solution

You require to prepare 200 uL of each protein at a concentration of 1mg/mL for an upcoming experiment. Compute the recipe to make each desired solution at the above concentration and volume.

  Understanding the perception and sensation.

Specify whether the taste of fresh water surprised you or not? Explain if your perception of coarseness change or not?

  Hearts of mammals homologous or analogous structures

Fish, reptiles, and mammals are descendents of a common vertebrate ancestor that had a heart. Fish hearts have two chambers, most reptile and amphibian hearts have three chambers.

  Find numbers of offspring with genotypes

Determine what would be the forecasted numbers of offspring with these four genotypes if the parental generation had been  AAbb  and  aaBB  instead of  AABB  and  aabb ?

  Homologous chromosomes in the cell during g1

based on diploid cell that has 28 chromosomes in G1 of interphase, Discuss how many pairs of homologous chromosomes are in the cell during g1?

  Find out the length of a dna molecule in inches

find out the length of a DNA molecule in inches

  Question about stem cells

Assume you have isolated a cell population from bone marrow using as newly discovered CD antigen. You believe that this population has hematopoietic potential.

  Compare the skull of vertebrates

Compare the skull of each of the following vertebrates, Sarcopterygians, Labyrinthodont amphibians, Snake and Therapsida. You should include similarities and differences in the neurocranium.

  How a primary oocyte gives rise to

how A primary oocyte gives rise to

  Detailed diagram of dna synthesis

Create a detailed diagram of DNA synthesis. With your diagram list the enzymes involved in the process and their respective functions.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd