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Making reaction

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Reference no: EM1383786

Assume you are given a primer with a concentration of 1.4 mg/ml, RNA concentration of 0.8 mg/ml, 1M MgCl2, 10mM dNTP mix, 10x buffer, 10 units/ul of reverse transcriptase. Describe how to make the following reaction:

5mM MgCl2

1x buffer

1mM dNTP mix

10ng of primer 1

10ng of primer 2

1ug of Rna

0.25 units of reverse transcriptase

Final reaction 20ul

Reference no: EM1383786

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