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Substance A is being investigated to see if it is an enzyme. When substance A is mixed with substance B a reaction takes place. A control experiment is conducted using a sample of A which has been boiled.
(a) Why is boiling used as a control?
(b) If the reaction still worked after A had been boiled, what might be your interpretation?
(a) Boiling denatures enzymes. If a substance still works after boiling, it cannot be an enzyme.
(b) If the reaction still worked after A had been boiled, either A is not an enzyme or, if it is, it is not necessary for the reaction.
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