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The Intine Layer
As soon as the tetrads release the microspores, the inner layer of the pollen wall (intine) is formed. Proteinaceous lamellae are embedded in the matrix of the intine, concentrating around the germpore. The proteins incorporated into the intine are contributed by the cytoplasm of the gametophyte, i.e., pollen cytoplasm. In members of the Malvaceae and several other plants, the proteinaceous lamellae are dispersed in the intine without coming in contact with the exine or pollen cytoplasm.
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describe the importance,principles,techniques and uses of microscopy
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