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Define Popularity of Chromatography - Basic Separation Technique?
Chromatography is popular because:
(a) minute quantities can be analysed qualitatively and quantitatively,
(b) the equipment is easy to use and the operation is very simple, and
(c) the results obtained are reproducible.
Basically, all chromatographic systems consist of two phases, a mobile phase and a stationary phase and molecules separate because they differ in their distribution between these two phases. The stationary phase is the sorbent and the mobile phase is the analysis by percolation of fluid through a body of porous rigid material irrespective of physiochemical process that leads to the separation of substances.
Bohr Effect Another important influence is the pH. Increase in carbon dioxide or other acids lowers the pH of plasma and shifts the dissociation curve to the right. At high ca
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a solution of 5mMol/L CaCl2 is separated from a solution of 1 micromole/L CaCl2 by a membrane that is selectively permeable to Ca+2 but is impermeable to Cl-1 what are the magnitu
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