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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Hepatitis B Vaccination against hepatitis B (Engerix-B - GlaxoSmithKline; Recombivax HB - Merck) is now a routine pediatric immunization in the US. It is recommended for previo
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why is obelia considered to be of special interest in Zoology as an animal showing an intermediate grade of organisation
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Explain Fosamprenavir calcium It is a prodrug of amprenavir, was recently approved by the FDA for use in HAART. In patients who have not lastly been treated with a protease inh
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As coenzymes participate in a variety of functions, they can be classified broadly into two groups: i) Hydrogen transferring coenzymes, and ii) Group transferring coenzym
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