Genomic dna fragments, Biology

Assignment Help:

You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.

If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.

 


Related Discussions:- Genomic dna fragments

Treatment of diarrhea, Treatment of diarrhea Loperamide (Imodium, and o...

Treatment of diarrhea Loperamide (Imodium, and others), an over-the-counter synthetic opioid (4-mg loading dose, then 2 mg orally after each loose stool to a maximum of 16 mg/d

What is endocrine system explain, What is Endocrine System explain ? Th...

What is Endocrine System explain ? The endocrine system consists of glands, organs that secrete hormones. Endocrine glands are tissues that produce secretions within the body,

Explain technologicai advances of clinical dietitian, Explain TechnologicaI...

Explain TechnologicaI advances of clinical dietitian TechnologicaI advances in nutritional  support for the critically ill have enhanced the clinical dietitian's role. In the

Explain about the toxicity of pyridoxine, Explain about the Toxicity of pyr...

Explain about the Toxicity of pyridoxine? Though toxicity related to pyridoxine intake are rare, but use of high doses of pyridoxine for the treatment of pre-menstrual syndrom

How many different genotypes can the individual present, Considering a pair...

Considering a pair of homologous chromosomes containing a gene having two different alleles how many different genotypes can the individual present? If a gene of a diploid spec

Pulmonary function tests - diagnostic tests, Pulmonary  Function Test: ...

Pulmonary  Function Test: Pulmonary functions  tests (PFTs) provide information about a clients manifestation by measuring lung volumes, lung mechanics, and diffusion capabili

Why we use histamine in consciousness, Q. Why we use Histamine in conscious...

Q. Why we use Histamine in consciousness? Histamine is involved in controlling the level of consciousness during waking. The level of histamine diminishes during sleep and its

Plants, why roots grow downword

why roots grow downword

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd