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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
The physician orders 2 liters of lactated ringers over 16 hours. The drip factor of the tubing is 20 gtt/cc. What will be the flow rate? a. 33.33 or 34 gtt/min b. 41.66 or 42
Locomotor Organelles The protozoan locomotor organelle may be Flagella, Cilia or Pseudopodia. These are of considerable value in classification of protozo
In vivo imaging in psychiatry To illustrate the ingenious applications to which in vivo imaging can be put, consider the use of PET in the study of hallucinations by Frith and
The Word 'disaster' is believed to be originating from the French word 'Disastre' which is a combination of two words 'des' meaning bad and 'astre' meaning star. Thus this term ref
Classification of the phylum protozoa
Determine what the abnormal levels of AFP signal Abnormal levels of AFP may signal the following: Open neural tube Defect (ONTD) Down syndrome Other chromosoma1
Visual Field Examination Russel et. al include a formal visual field examination using a parameter as part of their assessment procedure. Tests in the expanded version include
Source of Cells for Regeneration It had been considered earlier that interstitial cells constitute a reserve of undifferentiated cells that provide the cellular material for
all about excretion
dhow is the structure of connective tisse linked to their function
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