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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Tricks of learn a classification (molusca,arthopoda,prifera etc.)
excretory product of marine teleost
What is Micro-Endodontic Surgery? Combines of the magnification and illumination provided by the microscope with proper use of new micro-instruments.
Drawbacks of Free Food Items for diabetics patients In this context, it is important to note that a patient can have unlimited servings of 'free foods'. These are foods which d
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Aeration is the process on which water and air come into contact. It is carried out by spraying water in the form of spray or fountain over air. Aeration achieves the following obj
Explain Nevirapine and its adverse effects Nevirapine (NVP,Viramune) - Nevirapine is most effective at raising CD4 cell counts and lowering viral load when combined with 2 NRTI
Select all that are true/correct: Stable atoms have no vacancies in their outer shell. Hydrogen is more stable than carbon Helium is more stable than carbon Helium is more stable t
Aerotolerant anaerobes are incapable of aerobic respiration, but can nevertheless grow in the presence of air. Many lactic acid bacteria fall into this category. They can only gene
There are two main sources of pollution: (1). Natural Sources: (a) Volcanic ash (b) Marsh gas (CH4) released from decay of organic matter.
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