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You have an extensive library of genomic DNA fragments, prepared by the use of restriction nucleases. You know their sequences and sizes and you have an assay to reliably separate and identify them by electrophoresis on agarose gels. It has been reported that a newly isolated protein forms tight complexes with some of the DNA fragments.
If you can identify those DNA fragments, you might be able make a very educated "guess" as to the identity of the gene that is regulated by the protein. You do not yet have the sequence and structural information about the protein, so bioinformatics approaches are not going to be especially useful. How would you proceed? Be clear as to what type of assay(s)) you would do, and specifically how you could use the protein, and an antibody to the protein to identify candidate DNA fragments/genes.
Which of the following statement is true regarding the replication of DNA strands? A. The products of replication include one double helix made exclusively of parental DNA and
Parturient paresis (milk fever, hypocalcaemia) Parturient paresis is an acute to peracute non-febrile disease, which occurs in diary cows and buffaloes usually around the t
Organ level -Level of body organization When organs work together to perform a specific function we have the highest level of body organization i.e. the organ system level of bo
are protozoans primitive to life .
In a chemical reaction the free energy change (/\Go') is -40 jk/mol and the entropy factor T/\S is 15 kj/mol. the enthalpy change /\H for the reaction is a. 25 kj/mol b.-25 k
Each ribosome having of two subunits a small subunit and a large subunit, every of that is a multi component complex of rRNAs (ribosomal RNAs) and ribosomal proteins t
Organic Tricuspid Regurgitation Here the valve is anatomically abnormal. Etiology of Organic tricuspid regurgitation: 1) Rheumatic 2) Non rheumatic 1. Infective e
Behavioural Adjustments When faced with a sudden temperature change, most animals make behavioural responses that enable them to avoid extreme or lethal conditions. Among the
Define about the term Indicators? The easiest way to determine when the reaction is complete is by using indicators. An indicator is a compound that has a physical property (us
Diploidy and Haploidy :: In the chromosomal complement given species not all the chromosomes are different from each other .In fact these are in pairs ,i.e. every two chrom
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