Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

Compare & contrast replication, Compare and contrast replication, transcrip...

Compare and contrast replication, transcription, and translation based on the following criteria.   Criteria Replication Transcription

What do you mean by wound closure, Q. What do you mean by Wound closure? ...

Q. What do you mean by Wound closure? Generally the incisions designed for minimal exposure such as the H - shaped incision, do not require suturing, as the abutment and the tr

Respiratory care on admission of patient, Respiratory Care on Admission (Fi...

Respiratory Care on Admission (First two hours) Patient is incubated and ventilator-dependent.  Monitor blood gases hourly and take corrective action immediately.

Define general nutritional functions of minerals, Define General Nutritiona...

Define General Nutritional Functions of Minerals? We hear and talk about minerals almost everyday with regards to maintaining good health. But what are minerals and what functi

Hydrarch - kinds of succession, Hydrarch - Kinds of Succession When su...

Hydrarch - Kinds of Succession When succession takes place in a wet area, that is, succession progresses from hydric to mesic conditions. This can be further subdivided as:

Coronal disassembly-endodontics principles and practice, Coronal Disassembl...

Coronal Disassembly -If the existing restoration functionally designed well fitting and esthetically pleasing , -The access the pulp chamber during retreatment is better app

Attempted suicide - psychiatric emergencies, Attempted Suicide: Suicid...

Attempted Suicide: Suicide and homicide represent major source of morbidity and mortality in psychiatric practice.  Suicide means killing of  oneself.  An attempted suicide is

Trematodess, how do they adapt to their parasitic mode of feeding

how do they adapt to their parasitic mode of feeding

Sunlight used for sterilization, Q. Sunlight used for sterilization? It...

Q. Sunlight used for sterilization? It possesses appreciable bactericidal activity under natural condition. The action is primarily due to content of the ultraviolet rays; howe

Why does bark often die and break naturally, Why does bark often die and br...

Why does bark often die and break naturally? The bark is the mature periderm of the stem, branches and roots. It dies and breaks when these structures grow and thus the perider

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd