Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

What is right patellar tendon, What is right patellar tendon Which of t...

What is right patellar tendon Which of the following occur in response to an increase in the length of the right knee extensors in response to a quick tap applied to the right

Show the recessive phenotype, A woman who is heterozygous for a particular ...

A woman who is heterozygous for a particular X-linked recessive trait marries a phenotypically normal man. What percentage of their sons will show the recessive phenotype?

Transcription and processing of trna in prokaryotes , The rRNA transcriptio...

The rRNA transcription units in E. coli hold some tRNA genes which are processed and transcribed at the time of rRNA transcription. The other tRNA genes  happen  in  clusters  of

Ablation experiment, Ablation experiment  is an experiment designed to prod...

Ablation experiment  is an experiment designed to produce an animal deficient in one or the few cell types, in order to study cell lineage or the cell function. The logic is to mak

Types specimens of echpnodermata, Types specimens of echpnodermata Befor...

Types specimens of echpnodermata Before we begin the study of the selected specimens of the following echinoderms - Feather star, Brittle star, Sea urchin, Sea cucumber and Star

Define essential parts of photocolorimeter - monochromators, Define Essenti...

Define Essential Parts of Photocolorimeter - Monochromators? This is a means of selecting a sufficiently narrow waveband. Early colorimeters used glass filters that transmitted

Development of nutritional biochemistry, Development of Nutritional Biochem...

Development of Nutritional Biochemistry Nutrition,  in  general, and  particularly nutritional biochemistry, has  traveled a  long way since 1926, when nutrition was first reco

Molecular biology and technology, what are protein? What is the constituent...

what are protein? What is the constituential uni. Of protein? Briefly explain the various forms of protein?

Defination and use of stem cell, Stem cell research has been promoted as a ...

Stem cell research has been promoted as a future medical cure-all for a host of diseases, including Alzheimer's and diabetes, as well as a potential breakthrough for organ replacem

What is the meaning of paraplegia, What is the meaning of Paraplegia Pa...

What is the meaning of Paraplegia Paraplegia (from the Greek para, "alongside of," and plegia,  "stroke") is a condition in which both lower limbs are paralyzed (quadriplegia i

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd