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Define the Utilization of Most Probable Number Techniques?
The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.
Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.
There is a population of 100 frogs. 20 are heterozygotes, 30 are homozygous recessive, 25 are homozygous dominate. What is the frequency of the dominate allele? The recessive allel
Coenzyme A Coenzyme A is derived from the vitamin pantothenic acid. This is abbreviated as CoA. This can be divided into two components, adenosine 3,5-diphosphat
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The Word 'disaster' is believed to be originating from the French word 'Disastre' which is a combination of two words 'des' meaning bad and 'astre' meaning star. Thus this term ref
Foot as Burrowing Organ The Scaphopoda that usually have burrowing habit, possess foot suitably modified for burrowing purpose. The conical and protrusible foot of Dentalium
I nfe c tio u s laryngotracheitis It is a slow, laterally spreading respiratory disease of chickens, pheasants, peafowl and turkeys caused by a herpes virus with high morb
Acute Myocardial Infarction : Patients with acute non-Q myocardial infarction may need urgent intervention as indicated for cases of unstable angina.
Limiting Factor - Ecosystem In all ecosystems one factor, usually abiotic, limits the growth of organisms and is therefore called a limiting factor. The limiting factor is one
Q. Function of Noradrenaline in consciousness? Increased level of noradrenalin is implicated in wakefulness. Locus coeruleus noradrenergic neurons decrease their rate of firing
Obturator - Ovule An obturator is an outgrowth of the placenta or funicle or integument or style near the micropyle. It is presumed to guide the pollen tube to the micropyle.
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