Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

Explain the universality of the genetic code, How does the universality of ...

How does the universality of the genetic code make the recombinant DNA technology possible? The universality of the genetic code refers to the fact that all living beings have

Explain synaptic transmission between neurons, Q. How does synaptic transmi...

Q. How does synaptic transmission between neurons take place? The propagation of the action potential along the axon reaches the region immediately anterior to the presynaptic

Chromosomes/genes/heredidty, Plot the amount of DNA in the nucleus of a spe...

Plot the amount of DNA in the nucleus of a spermatogonia from the G1 stage prior to the first meiotic division through the completion of meiosis. Label each of the major stages of

Phylum arthropoda - classification of multicellular animals, Phylum Arthrop...

Phylum Arthropoda - Classification of Multicellular Animals Earlier you known how the coelom evolved and the body of the coelomates got segmented leading to evolution of segme

Define biochemistry, Biochemistry : The chemistry  of  living organisms ...

Biochemistry : The chemistry  of  living organisms that covers all  the chemical reactions occurring  in our body.

Introduction to ecology, Ecology is a interdisciplinary branch of science t...

Ecology is a interdisciplinary branch of science that deals with inter relationship between living organism and their environment. The term ecology (from greek, oikos-meaning ho

Define deuterostomes in animal kingdom, Define Deuterostomes in animal king...

Define Deuterostomes in animal kingdom? Coelomate animals are thought to have separated into two divergent lines of evolution in terms of the way embryos develop. In the embryo

Techniques of closed heart surgery, Techniques :  Depending on the type of...

Techniques :  Depending on the type of surgery planned, the heart may be approached through median sternotomy, left or right thoracotomy. The problems encountered could be due to

Energy requirements of school children and adolescents, Determine Energy re...

Determine Energy requirements of school children and adolescents? Table depicts the energy requirements of school children and adolescents,  as recommended by ICMR (1990).

What are the cytoplasmic structures present in animal cells, Q. What are th...

Q. What are the main cytoplasmic structures present in animal cells? The main cytoplasmic structures of the cell are the centrioles, the cytoskeleton, mitochondria, peroxisomes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd