Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

What is benedict's test and its principle, What is Benedict's Test and its ...

What is Benedict's Test and its Principle? This test is answered by all reducing sugars with a free aldehyde or ketone groups. Monosaccharides possess a free aldehyde or ketone

Myocarditis, Myocarditis Myocarditis is inflammation of the myocardiu...

Myocarditis Myocarditis is inflammation of the myocardium, of the heart muscles. i) Causes Infection: . Wrus, bacteria, fungus, parasites Non-infection: Radi

State the success which signifies optimum health, Success which signifies O...

Success which signifies Optimum Health The features or clinical conditions which are representative of this group are: i) No pain or tenderness in the implant upon function.

Explain dextran, Dextran Dextran is a high molecular  weight polysaccha...

Dextran Dextran is a high molecular  weight polysaccharide having  α1→ 6,  α1→ 3 linked glucans. It is produced by the bacterial fermentation of sucrose by  Leuconostoc dextran

Define the concepts of lipids, Define the Concepts of Lipids? You have ...

Define the Concepts of Lipids? You have studied the classification of lipids in your theory lesson. Lipids, you learnt, are classified as simple, compound or derived lipids on

Define historical example for dynamical network, Define Historical example ...

Define Historical example for Dynamical Network? John Tyson constructed a nonlinear differential equation model representing the majority of the network of biochemical pathways

Cytokinesis in an animal cell, Cytokinesis in an animal cell: Splitting...

Cytokinesis in an animal cell: Splitting of the cell is called cytokinesis which starts at telophase. In animal cells, microtubules form a furrow in a ring around the cell.

Define estimation of iron in any solution - minerals, Define Estimation of ...

Define Estimation of Iron in any Solution? Iron can be determined calorimetrically. The principle is described next. Principle The iron is determined in the solution or d

Explain environmental resistance and the population growth, What is the rel...

What is the relationship among environmental resistance and the population growth according to the biotic potential curve and the real population growth curve? The difference

History of protoplasm, HIS T O R Y - Protoplasm was first seen b...

HIS T O R Y - Protoplasm was first seen by Corti, 1772 F . Dujardin called it "Sarcode" & observed it in animal cell Term "Protoplasm" was given by J.E. Pu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd