Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

Explain about the vitamin d, Explain about the Vitamin D? Vitamin D is ...

Explain about the Vitamin D? Vitamin D is a generic term and indicates a molecule of the general structure with  rings (A, B, C, D), as you may have noticed in Figure B. The ri

Poultry and duck diseases-infectious laryngotracheitis (ilt), Infectious la...

Infectious laryngotracheitis (ILT) Infectious laryngotracheities, caused by fowl Gallid Herpesvirus 1 belonging to the family Herpesviridae, is a quickly spreading acute res

What is the production of the rna, Q. What is the production of the RNA cal...

Q. What is the production of the RNA called and what is the enzyme that catalyzes the procedure? The making of the RNA from information contained in the DNA is called transcrip

Molecular biology, how biochemical evidence proof DNA as a genetic material...

how biochemical evidence proof DNA as a genetic material?

What are nerve function, The major function of the nerve cells of the body ...

The major function of the nerve cells of the body is contact. This is accomplished by passing electrical and chemical messages from neuron to neuron or from neuron to one other goa

Interaction of pyridoxine with ascorbic acid and leucine, Define Interactio...

Define Interaction of pyridoxine with Ascorbic acid and Leucine? Ascorbic acid: Vitamin B 6 metabolism increases with. Higher levels of vitamin C intake. Whole blood a

Is fecundation in mammals internal or external, Is fecundation in mammals i...

Is fecundation in mammals internal or external? Fecundation in mammals is internal, with copulation. In the contemporary world human technology is able to endorse artificial e

Explain the term- latent squint, Explain the term- Latent Squint (Anisophor...

Explain the term- Latent Squint (Anisophoria or Heterophoria) This eye condition occurs when the balance of extra-ocular muscles is altered. There is a tendency of the eye to

Kind of tetrasporic embruo sac, what are the kinds of tetrasporic embryo sa...

what are the kinds of tetrasporic embryo sac...detail information

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd