Define the utilization of most probable number techniques, Biology

Assignment Help:

Define the Utilization of Most Probable Number Techniques?

The MPN technique (multiple tube technique) can be used to determine the presumptive coliform count in the food. The sample is homogenized adequately and decimally diluted in saline solution or peptone water. Measured portion (e.g. 1 ml) of the solution and dilutions is transferred into multiple tubes (3 or 5) of selective and differential medium e.g. LST and incubated at 35°C for 48 hours.

Production of gas in inverted Durham tube by lactose fermentation indicates coliforms. By using standard MPN tables, as given herewith, MPN/ml or MPN/g of food samples is derived from number of positive tubes at 3 successive dilutions.The method was given by Mc Crady in 1915 and is statistically based. Three tubes or five tubes method can be used by inoculating 3 serial dilution of the sample into 9 or 15 tubes of appropriate medium respectively. The number of organisms in the original sample is determined by using standard MPN table as we have already studied in the last practical.


Related Discussions:- Define the utilization of most probable number techniques

Skeleton, what is the biological significance of skeleton

what is the biological significance of skeleton

Microtubules, MICROTUBULES Discovered by De Robertis and Franchi ...

MICROTUBULES Discovered by De Robertis and Franchi . Term given by Slautterback . These are hollow structures, consists of tubulin protein. Each protein diamer

Spina bifida cystica, Spina Bifida Cystica There is a protrusion of me...

Spina Bifida Cystica There is a protrusion of meninges and spinal cord a) Meningocele- it is relatively uncommon lesion (4-5 per cent). In this there is protrusion of me

Define antioxidant activity of manganese, Define Antioxidant Activity of ma...

Define Antioxidant Activity of manganese? As Mn is a component of mitochondrial Superoxide Dismutase (SOD), it can protect against oxidative damage. In-vitro experiments have i

Effects of cardiac output and hormones, Excluding the effects of cardiac ou...

Excluding the effects of cardiac output and hormones, describe the other factors that may affect blood pressure and blood flow in a middle-aged man who is exercising in an aerobics

Endothelium - ovule, Endothelium - Ovule In plants bearing unitegmic o...

Endothelium - Ovule In plants bearing unitegmic ovules, the nucellus degenerates during early stages of ovule development and the embryo sac comes in contact with the innermos

What are the animal pole and the vegetal pole of egg, Q. What are the anima...

Q. What are the animal pole and the vegetal pole of the vertebrate egg? The animal pole of a telolecithal egg is the portion of the egg with little vitellus it is opposite to t

Variation of the photosynthesis effectiveness, Q. What is an example of a l...

Q. What is an example of a lab experiment that shows the variation of the photosynthesis effectiveness in relation to different photic energy frequencies to which the reaction is e

Define the principles of periapical surgery (pas), Define the Principles of...

Define the Principles of Periapical Surgery (PAS)   1. Avoid horizontal, sever angled vertical incision Because the collagen fibers of the mucoperiosteum are parallel to the to

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd