Electrophoresis gel to load 60, Biology

Assignment Help:

Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing agent, and incubate 10 minutes at 70°C. How much of this "gel-ready" solution must you pipette into a well of your electrophoresis gel to load 60 ?g of protein?


Related Discussions:- Electrophoresis gel to load 60

Lab report, Why making a smoke print of a leaf

Why making a smoke print of a leaf

Explain reality theory, Reality Theory Developed in  the 1960s by  Will...

Reality Theory Developed in  the 1960s by  Willian glasser,  a  psychiatrist,  reality  therapists  view human nature in terms of behaviour. They believe that human behaviour i

Intermediate filaments - role of cytoskeleton structures, Intermediate fila...

Intermediate filaments - Role of Cytoskeleton Structures These filaments are intermediate in size among microtubules and microfilaments and are 10 nm in diameter. Five classes

State cryptobiosis and explain the function of it, State Cryptobiosis and e...

State Cryptobiosis and explain the function of it. A dormant, or suspended, state of animation which allows some animals to survive severe conditions like extremely low tempera

Define endurance capacity by standard running tests, Define Endurance capac...

Define Endurance capacity by standard running tests? Endurance capacity, on the other hand, is measured by standard running tests in which time to exhaustion is taken as the en

Deficiency diseases-thiamine deficiency (hypothiaminosis), Thiamine deficie...

Thiamine deficiency (hypothiaminosis) Thiamine deficiency is characterized by signs of nervous system. The primary  thiamine deficiency is not common in animals. However, seco

What is the effect of increase temperature, 1) What is the effect of increa...

1) What is the effect of increase temperature on a non enzyme catalyzed reaction? 2) What are the effects of increased temperature on an enzyme catalyzed reaction?

Determine the concept of imitation in the learning process, Determine the c...

Determine the concept of  imitation in the learning process The concept of  imitation in the learning process is also a key developmental concept for infants and young children

Submerged stage - hydrarch, Submerged Stage - Hydrarch This habitat wh...

Submerged Stage - Hydrarch This habitat which is now shallower and is richer in nutrients and where light is available up to a certain depth, becomes suitable for the growth o

Embryonic development, Embryonic Development The embryonic stage exte...

Embryonic Development The embryonic stage extends from the second week by the 8th week and is charactrised by formation of placenta, the development of internal organs and ap

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd