Electrophoresis gel to load 60, Biology

Assignment Help:

Assume your tilapia fish muscle homogenate has a protein concentration of 5769 ?g/ml. You take 32.5 ?l of this fish homogenate, mix it with 12.5 ?l sample buffer and 5 ?l reducing agent, and incubate 10 minutes at 70°C. How much of this "gel-ready" solution must you pipette into a well of your electrophoresis gel to load 60 ?g of protein?


Related Discussions:- Electrophoresis gel to load 60

Are bacteria the only prokaryotic beings, Q Are bacteria the only prokaryot...

Q Are bacteria the only prokaryotic beings? Prokaryotic beings are classified into two big groups: bacteria and archaebacteria (this last also known as eubacteria). Compared

Animal biodiversity, Discuss why Obelia is considered to be of special inte...

Discuss why Obelia is considered to be of special interest in Zoology as an animal showing an intermediate grade of organization.

Which are the plant tissues, Which are the plant tissues responsible for th...

Which are the plant tissues responsible for the supporting of the plant? The plant supporting tissues are the collenchyma and the sclerenchyma. The collenchyma is made of li

X-ray chest and ecg, X-ray Chest It is helpful in assessing heart size...

X-ray Chest It is helpful in assessing heart size. One should look for presence of pericarditis, pulmonary oedema or pulmonary congestion. ECG One should look for pro

Explain about sex chromosomes, What is the other name given to sex chromos...

What is the other name given to sex chromosomes? Sex chromosomes are also known as allosomes (the other chromosomes that are not sex chromosomes are known as autosomes).

Determine the symptoms of cholera, Determine the Symptoms of Cholera  A...

Determine the Symptoms of Cholera  An infective dose of around one million organisms should be ingested to cause illness. The bacteria adhere to the small intestine wall, where

Name main parts into which a neuron can be divided, What are the three main...

What are the three main parts into which a neuron can be divided? What are their respective functions? The three mains parts into which a neuron can be didactically separated a

The probability of an individual in the trihybrid, The probability of an in...

The probability of an individual in a trihybrid F2 generation showing the dominant phenotype for just two traits?

, comparitive table to explain the differences between the animals in chor...

comparitive table to explain the differences between the animals in chordate classes

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd