Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

Define the potassium ion impermeability, At 1 AM, an impermeable membrane s...

At 1 AM, an impermeable membrane separates a 1 liter solution of 2M KCl in the left compartment from a 1 liter solution containing both 1M NaCl and 1M KCl in the right compartment.

Define method used for capsular staining - anthony staining, Define method ...

Define method Used for Capsular Staining - Anthony Staining Method? Another method used for capsular staining is Anthony staining method, devised by E.E. Anthony in 1931. The m

What are pentoses, What are pentoses? What are the roles of pentoses in DNA...

What are pentoses? What are the roles of pentoses in DNA and RNA molecules? Pentoses are carbohydrates made of five carbons. The DNA molecule is made of a sequence of molecu

Shapes - types of nursing units, Shapes: Nightingale Ward  In the 1...

Shapes: Nightingale Ward  In the 1940s,  this traditional nursing unit was based on  the long rectangle pavilion type ward and self-contained unit. This concept is establis

Gene targeting in es cells, Gene targeting in ES cells: In some of the li...

Gene targeting in ES cells: In some of the livestock species pro-nuclear injection cannot be used to modify the animals' own genes due to various reasons especially non availabil

Transmission electron microscopy, Transmission Electron Microscopy The so...

Transmission Electron Microscopy The source of illumination in a TEM is a tungsten filament that emits electrons. A vacuum is created to prevent the collision of electrons with a

Define ailing and failing, Define ailing and failing Clinically unhealt...

Define ailing and failing Clinically unhealthy implants are classified as "ailing" or "failing". It is necessary to distinguish between an ailing versus a failing implant to de

What are enzymes?, Enzymes are proteins that are catalysts of chemical reac...

Enzymes are proteins that are catalysts of chemical reactions. From Chemistry, it is called that catalysts are non-consumable substances that decrease the activation energy essenti

Can be made from set of 20 naturally occurring amino acids, How many differ...

How many different molecules composed of (A) two (B) three, and (C) four amino acids, linked together by peptide bonds, can be made from the set of 20 naturally occurring amino aci

How do taenias obtain food and make gas exchange, Q. How do taenias obtain ...

Q. How do taenias obtain food and make gas exchange? The Tapeworms have sucking and hooks structures on their heads (scolex) that fixate the parasite in the gut wall these stru

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd