Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

Carrier ions like na+ facilitate the absorption of substance, Carrier ions ...

Carrier ions like Na+ facilitate the absorption of substances like: 1. amino acids and glucose 2. glucose and fatty acids 3. fatty acids and glycerol 4. fructose and so

The principle of dna cloning, Assume an experimental target that is to make...

Assume an experimental target that is to make vast amounts of a particular DNA fragment in pure form from a combination of DNA fragments.  Whereas the DNA fragments can be introduc

What is endocrine system explain, What is Endocrine System explain ? Th...

What is Endocrine System explain ? The endocrine system consists of glands, organs that secrete hormones. Endocrine glands are tissues that produce secretions within the body,

Explain the life cycle of water, Explain the life cycle of Water? The ...

Explain the life cycle of Water? The water cycle , or hydrologic cycle, is one of the most important processes to living organisms on Earth. Consider the following facts:

Phylum colentrata, In phylum colenterata if tissues are present in the body...

In phylum colenterata if tissues are present in the body of an organism then why organs are not present as group of tissues makes an organuestion..

Define modulation of transcription of cell cycle proteins, Modulation of th...

Modulation of the transcription of cell cycle proteins? The 1,25-(OH),D  compound (calcitriol), is present in the blood complexes to the vitamin D-binding  protein, a specific

What is the difference among living and non-living things, What is the diff...

What is the difference among living and non-living things? Living things obtain and use energy. They also grow throughout their lifetime. Living this is made of cells. Living t

Classification of proteins based on chemical properties, Classification of ...

Classification of proteins based on chemical properties? Depending on their chemical properties and optical activity, the amino acids in proteins are classified under the follo

Differential reinforcement of other behaviour, Differential reinforcement o...

Differential reinforcement of other behaviour (DRO) This is used to decrease frequent behaviour by reinforcing any behaviour other than the undesired one. An instance would be r

What are the basic constituents of the cell membrane, What are the basic co...

What are the basic constituents of the cell membrane? The cell membrane is produced of lipids, proteins and carbohydrates. The membrane lipids are phospholipids, a special t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd