Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

A balanced diet must contain enough energy, A balanced diet must have enoug...

A balanced diet must have enough energy to meet the body's needs. What else must it have?    In addition to sufficient energy, a balanced diet must have proteins, car

What are the steps in bacterial infection, It contain four steps. They are:...

It contain four steps. They are: Attachment to host Proliferation Invasion of host tissue Toxin-induced damage to host cell

Goals of dietary treatment for dyslipidemia, Q. Goals of Dietary Treatment ...

Q. Goals of Dietary Treatment for dyslipidemia? The goals of dietary management (alone or conjunction with exercise or with lipid lowering drugs) are to reduce the total fat, s

How vitamin k used to prevents bone loss, How Vitamin K used to Prevents bo...

How Vitamin K used to Prevents bone loss? Vitamin K is known to inhibit bone loss through inhibiting effect on osteoclast formation. Thus, adequate levels of vitamin K must be

What are the luria''s methods and theories, What are the Luria's methods an...

What are the Luria's methods and theories Adhering to our theory that the Luria Nebraska bears little resemblance to Luria's methods and theories, there seems little point in i

Define tests to measure muscular strength and endurance, Define Tests to Me...

Define Tests to Measure Muscular Strength and Endurance? It is important to first understand the difference between muscular strength and endurance. To know the muscular streng

Effects of flow limiting stenosis on blood flow, 1) The presence of epicar...

1) The presence of epicardial coronary artery stenosis caused by artherosclerotic plaques is by far the most frequent angiographic finding in any cardiac ischaemic syndrome. 2)

How cleaning agents works, Q. How Cleaning agents works? Food particles...

Q. How Cleaning agents works? Food particles and other debris provide good nutrients for the nlicroorganisms to grow. In fact, the food particles protect microorganisms d

Explain the appearance of a single e. coli colony, Describe the appearance ...

Describe the appearance of a single E. coli colony. Why can it be considered genetically homogenous?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd