Culture conditions, Biology

Assignment Help:

Culture Conditions

The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.

A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.


Related Discussions:- Culture conditions

Genetic defect in pyruvate dehydrogenase, Genetic Defect in Pyruvate Dehydr...

Genetic Defect in Pyruvate Dehydrogenase A defed in any of  the protein subunits of PDH can result in decrease or complete loss of  activity. Severe cases are usually fatal. Sy

Oxygen transport in blood, Oxygen Transport in Blood All four respirat...

Oxygen Transport in Blood All four respiratory pigments are adapted to load and unload oxygen effectively in I the habitats where they have evolved, whether animals live on la

Attempted suicide - psychiatric emergencies, Attempted Suicide: Suicid...

Attempted Suicide: Suicide and homicide represent major source of morbidity and mortality in psychiatric practice.  Suicide means killing of  oneself.  An attempted suicide is

Applied Biology, Which of the branches of applied biology ?

Which of the branches of applied biology ?

Define larval stages of fasciola hepatica fasciola hapatica, Define the Lar...

Define the Larval stages of fasciola hepatica? Fasciola hapatica reproduces both sexual and asexually. Cross-fertilization is of common occurrence. Copulation takes place in t

General nursing responsibilities in diagnostic procedure, GENERAL NURSING R...

GENERAL NURSING RESPONSIBILITIES IN DIAGNOSTIC PROCEDURE Let us understand what are the general responsibilities of nursing personnel while assisting in diagnostic procedure

Features of plant hormones, Features of Plant Hormones The following a...

Features of Plant Hormones The following are the important features of the role of plant hormones in growth and development: The hormone may initiate one response

Fin Fish, What are five fin fish found in the North-East United States?

What are five fin fish found in the North-East United States?

A light microscope with unlimited resolution, Theoretically you can build a...

Theoretically you can build a light microscope with unlimited magnification, but can you build a light microscope with unlimited resolution? Why or why not? (Hint: what really cont

Explain the role of solutions, Explain the role of solutions? Solution...

Explain the role of solutions? Solutions:  A solution consists of a liquid solvent plus substances dissolved in it, called solutes. Solutes may be ions, atoms, or small molec

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd