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Culture Conditions
The culture vessels placed in suitable trays or racks, are stored on the shelves in a hygienically maintained culture room, under controlled conditions of light and temperature. If possible the relative humidity should not fall below 50% to prevent rapid desiccation of the medium. To minimize the infection of cultures during incubation, the culture room should be dust free. It is often provided with double doors and entry of people is restricted. The requirement for light and temperature varies considerably. The cultures of high altitude plants as well as those of desert species grow well at 25? c-28?c. However, induction of pollen embryogenesis requires a high temperature treatment (35? C). As mentioned earlier, in cultures even green tissues and shoots do not exhibit active photosynthesis and are largely heterotrophic. The differentiation of shoots from callus or explants and axillary shoot proliferation are favored by light.
A callus is defined as an unrecognized mass of tissue varying widely in texture, appearance and growth rate. Such cultures should be maintained in continuous diffuse light (1-3K lux) provided by fluorescent tubes. You should know that unorganized callus and freshly isolated protoplast cultures are often sensitive to light and are incubated in darkness. Total darkness is also favorable for rooting and for the initial stages of pollen and somatic embryogenesis.
Gene targeting in somatic cells: Since the pioneering work with sheep at Roslin cloning by nuclear transfer has now been exemplified in a number of other livestock species includ
Q. Show the principle parts of a mold? The principle parts of a mold are a web-like structure known as mycelium and the spore. The mycelium is often white and cottony and penet
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As heart failure sets in, there is activation of RAS (Fig 2.1). Adrenergic stimulation of beta-1 receptors in juxtaglomerular apparatus of the kidneys results in release of renin.
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Q. What is the usual biological function of the connective tissues? How is this function associated to the main features of its cells? The usual function of the connective tiss
What is Palliative Outflow Patch ? Brock described closed pulmonary valvotomy and infundibular resection using a punch on a beating heart. When both pulmonary arteries are not
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What is the Importance of vitamin B 12 Vitamin B 12 deficiency is manifest by the symptoms of pernicious anaemia (Addison-Biermer's disease). An irreversible atrophy of the
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