Gel shift assay, Biology

Assignment Help:

Gel shift assay is a technique by which one can determine whether a particular protein preparation contains factors which bind to the specific DNA fragment. At times when a radiolabeled DNA fragment is run on the gel, it shows the characteristic mobility. If it is first incubated with the help of cellular extract of proteins (or with the purified protein), any protein-DNA complexes will migrate from one place to another slowly than the naked DNA - a shifted band.


Related Discussions:- Gel shift assay

Determine what are the genotypes of the parents, 1. In chickens rose comb (...

1. In chickens rose comb ( R ) is dominant over single comb ( r ). A rose-combed male is mated to a rose-combed female. Eighteen chicks are produced, ten of which are rose-combed,

What is the difference between brain and cerebrum, What is the difference b...

What is the difference between brain and cerebrum? What are the main parts of these structures? The concept of brain, or encephalon, comprehends the cerebrum (mostly referred t

Zoology, give the discriptive account of nerilla which belongs to class a...

give the discriptive account of nerilla which belongs to class arciannelida in phylum annelida

Explain the following terms in detail - homology, Explain the following ter...

Explain the following terms in detail - Homology, Homologous ? Structures that have a similar evolutionary origin however different functions. The wing of a bat and a whale's fl

Molluscs., what are the economic and ecological importance of molluscs

what are the economic and ecological importance of molluscs

Phylum , General characteristics of phylum

General characteristics of phylum

List the requirements of implant materials, List the requirements of implan...

List the requirements of implant materials. a) Biologically compatibility: an ideal implant material will elicit mainly physiological reactions within the surrounding tissues (

Diestrus- estrous cycle, Diestrus- Estrous cycle This lasts 60 to 70 h...

Diestrus- Estrous cycle This lasts 60 to 70 hours during which functional regression of the corpora lutea occurs. The uteri are small, anaemic, and only slightly contractile.

Can you explain reticulum and the rumen, Q. Cows swallow their food once an...

Q. Cows swallow their food once and then this food goes back to the mouth to be chewed again. How can this phenomenon be explained? The food ingested by cows and other ruminant

What are the types of nutrients, Q. What are the types of nutrients? Explai...

Q. What are the types of nutrients? Explain the functions of nutrients? Types of nutrients  • Carbohydrates • Proteins • Fats • Minerals • Vitamins • Water Fu

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd