Gateway reaction-entry clone and destination vector, Biology

Assignment Help:

1530_Gateway Recombination reaction.png

(1) From the above gateway reaction (entry clone and destination vector);

(i) which plasmid will be selected for when transformed into E.coli(A or B) and why?

(ii) which antibiotic would you included in the selection?

(iii) which strain of E. coli would you transform the gateway reaction into?

(iv) which strain of E. coli do you need to maintain and amplify the entry clone

(v) which strain of E. coli do you need to maintain and amplify the destination vector.


Related Discussions:- Gateway reaction-entry clone and destination vector

What are the factors responsible for corneal hydration, What are the factor...

What are the factors responsible for corneal hydration? The factors responsible for corneal hydration are as follows: i. Stromal swelling pressure ii. Barrier function of

What is implant success, Implant success 1) An individual, unattached i...

Implant success 1) An individual, unattached implant is immobile when tested clinically. 2) A radiograph that does not demonstrate any evidence of perimplant radiolucency.

Gastrointestinal hormones, Gastrointestinal Hormones The three main ma...

Gastrointestinal Hormones The three main mammalian gastrointestinal hormones are secretin, gastrin and cholecystokinin (CCK). There are several other hormones all peptides. Th

Define effect micronutrients during pregnancy period, Define effect Micronu...

Define effect Micronutrients during pregnancy period? The need for many vitamins and minerals is increased during pregnancy. Since energy intake increases, the requirements for

Determine balanced diet for celiac disease, Determine Balanced Diet For Cel...

Determine Balanced Diet For Celiac Disease? Energy: initially the children are seen to be malnourished, thus 100-120 Kcal/kg body weight/day with gradual increase to 150 Kcal

Veterinary medicine, Veterinary medicine Veterinary medicine is a branc...

Veterinary medicine Veterinary medicine is a branch of science that deals with diagnosis, treatment and control of diseases in animals such as livestock, domesticated animals,

Implant system, In the early 1970's, Schroeder and his group designed a sin...

In the early 1970's, Schroeder and his group designed a single stage implant system known by the name of ITI Implant System. They demonstrated that one-stage implant can also resul

Acute phase reactants, ESR and CRP are elevated in almost all patients of a...

ESR and CRP are elevated in almost all patients of arthritis and carditis and rarely in patients with chorea. ESR should be repeated periodically as it is useful in following the c

Asexual reproduction, Which type of asexual reproduction takes place in mon...

Which type of asexual reproduction takes place in monocystis?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd