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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
COMPOSITION Proteins = 44-76%, Lipids = 20-53%, Carbohydrates = 1-8%, (Protein-Lipid ratio = 0.8 : 1 to 4 : 1) Most of lipid are "Phospholipids" which are amphipat
Transcribed regions The transcribed regions of the genes contain a number of regulatory elements that control exon splicing, mRNA transfer to cytoplasm translation, mRNA target
Founder effect The difference in gene pools amongs an original population and a new population founded by one or a few individuals randomly separated from the original population,
Q. What is Dyslipidemia or Hyperlipidemia? It has been known for over five decades now that dyslipidemia is associated with increased severity and prevalence of atherosclerosis
When coal is carbonized coke is formed along with coal gas. 1300 0 C Coal coke + coal gas
Iodine Iodine functions are essential components of the thyroid hormones thyroxine (T 4) and triiodothyronine (T3), which regulate the rate of energy metabolism. Absorbed iodi
Organic Molecules Some organic molecules in life are Carbohydrates and Lipids à C, H and O Proteins à C, H, O, N & sometimes S Nucleic acids à C, H, O, N & P
Difference between rod and cone cells
Ascariasis This disease is found in young calves causing digestive disturbances and poor growth. E t iology: It is caused by Toxocara vitulorum in cow and buffal
DISORDER S OF THE ADRENAL CORTEX - ( i ) ADDISON'S DISEASE : This disease is caused by the deficiency of mineralocorticoids and glucocorticoids. Its symptoms include l
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