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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
significance of radial symmetry
Q. Show types of hiatus hernia? Depending on the extent of herniation and placement of stomach, it could be of two types: 1, Para Oesophageal Hernia (oesophagus is in normal
The aqueous solution with the lowest pH is: Select one: a. 0.01 M HCl. b. 0.1 M acetic acid (pKa = 4.86). c. 0.1 M formic acid (pKa = 3.75). d. 0.1 M HCl. e. 10 t
What is the functionality of Lungs in human biology? The right lung consists of three lobes, and the left lung, two lobes. Both lungs hold between 5 to 6 L of gas, called the t
Contra Indication : It is absolutely contra indicated if their is more than trivial aortic regurgitation. Aortic aneurysm and severe aorto iliac disease are also contra indicatio
Q. What is the anatomical reason for the left bronchus to be more elevated than the right bronchus? Why in most cases of aspiration of foreign material by children is the object fo
Set Up of Phototherapy Unit Phototherapy generally consists of four to eight cool white, day bright, or special blue fluroescent tube lights covered by a plastic shield and p
Q. Nematode identity card. How are nematodes characterized according to examples of representing beings, type of symmetry basic morphology, , germ layers and coelom, digestive syst
differences between acoelomate,coelomate and pseudocoelomate
What is Cost Effective Analysis? Cost effective analysis: Cost effective analysis provides for the choice of least expensive strategy at the least cost. Here the outcome is mea
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