Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

Explain the solid-liquid equilibria, Explain the Solid-Liquid Equilibria? ...

Explain the Solid-Liquid Equilibria? A freezing-point curve (freezing point as a function of liquid composition) and a solubility curve (composition of a solution in equilibriu

Explain the natural history of coronary artery diseases, Explain the NATURA...

Explain the NATURAL HISTORY OF CORONARY ARTERY DISEASES (GAD)? The natural history of CAD is very important from the preventive point or view. Though the usual manifestations o

Cytoplasmic bridge formed during the conjugation of paramoec, Cytoplasmic b...

Cytoplasmic bridge formed during the conjugation of paramoec: The two paramoecia that undergo conjugation are called conjugants. During conjugation, the two conjugants com

What is tubal pregnancy, Q. What is tubal pregnancy? Many times fecunda...

Q. What is tubal pregnancy? Many times fecundation takes place in the Fallopian tubes in general the newly formed zygote is taken to the uterus where nidation and the embryonic

Types of passive transport, Simple diffusion Only comparatively small u...

Simple diffusion Only comparatively small uncharged or hydrophobic molecules (H2O, O2, and CO2, other gases, urea and ethanol)   cross   the lipid   bilayer   through simple

Explain typical components of a closed circulatory system, What are the typ...

What are the typical components of a closed circulatory system? The typical components of the closed circulatory system are the blood vessels within which blood circulates (vei

Determine the recombination frequency of genes, Why does the recombination ...

Why does the recombination frequency of genes vary with the distance between them in the chromosome? The farther the distance among the loci of two genes in a chromosome the h

How did gifford pinchot view nature, How did muir's impression of man's rel...

How did muir's impression of man's relationship with nature differ from Susan Fenimore coopers? And How did Gifford Pinchot view nature?

Life cycle of filarial worm, Life cycle of Filarial worm Filariasis : ...

Life cycle of Filarial worm Filariasis : It is popularly known as elephantiasis. It is caused by a nematode parasite, wuchereria bancrofti, and W.Malayi. Parasite : Wucher

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd