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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Q. Explain Nutritive and Non-Nutritive Sweeteners? Nutritive Sweeteners: We know some sweeteners like glucose, honey, molasses, fruit juice, dextrose, maltose, mannitol, sorbit
Female Gametophyte The development of a female gametophyte is initiated with the enlargement of one of the megaspores (usually the one close to the chalaza in a linear tetrad)
Q. Of which cells is the nervous tissue constituted? How is the generic function of this tissue related to the characteristics of the main cell type that forms it? The nervous
Characteristics of River Systems The basic function of the rivers is to convey surplus rain water from land to sea. Annually the rivers carry fresh water, equivalent to 25 cm
Adefovir dipivoxil (Hepsera) This phosphonate nucleoside analog, administered as the oral prodrug, inhibits replication of HBV, including variants resistant to lamivudine. Ade
Regulation of Ammonia Assimilation Heterotrophic bacteria like Escherichia coli and Klebsiella aerogenes induce the operation of GS-glutamate synthase pathway of ammonia assim
phase of alpha taxonomy
What is Multiple Alleles ? Obviously, diploid organisms, such as humans can have only two different alleles for a certain gene locus. However, new alleles are continuously bein
Q. What are the sources of dietary fibre in our diet? The sources of dietary fibre include whole grain cereals, legumes, whole pulses, leafy vegetables, vegetables like peas,
Minerals :- Iron Food Source Cereals, legumes, meat contamination from iron utensils and soil Nutritional Functional role Essential nutrient: Deficiency lead
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