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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
In developing countries because of poor compliance, poverty, ignorance, primary and secondary prevention strategies are difficult to implement properly. Also in western countries (
Which of the following terms best describes calcium metals? Check all that apply. Molecule, element, matter, and compound
Explain brifly Ribosomes, Endoplasmic Reticulum, Golgi Apparatus ? Ribosomes, Endoplasmic Reticulum, Golgi Apparatus : The cytoplasm contains many ribosomes, which are sphe
Define Dark Field Microscope In this microscope, condenser system is modified, so that only reflected and refracted light by specimen forms an image. Here, the object is bright
Why is rubella during gestation a threat to the fetus? If occurring during gestation rubella is a dangerous disease due to the virus crosses the placenta and contaminates the f
What is a helix?
Hydrophilicity Plot is a computer plot which checks the relative summed hyrophobicity or hydrophilicity of adjacent amino acid sidechains (usually within a moving window of about
#question.sir please explain amoeba as in example of protozoa
COMMON DISEASE OF CAMELS - 1 . SURRA - Surra = Rotten. Spread by biting & blood sucking flies, dropping neck, half closed eyes are common symptoms. 2 .
Define Bone growth and development calcification of osteoid tissue? Vitamin D participates in metabolic processes associated with bone growth and development. It is involved i
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