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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
How Environmental temperature affect requirement of protein? Environmental temperature: As environmental temperatures rise or fall above or below the range of thermic neutralit
Q. How is the nervous system of nematodes organized? Where are the neural chords located in their body? Roundworms have a ganglial nervous system with an anterior neural ring r
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Define Conditions associated with increased need for Vitamin A? Conditions and populations associated with increased need for vitamin A includes young children particularly t
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