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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
A cell in G1 of interphase has 12 chromosomes. How many chromosomes and DNA molecules will be found per cell when this original cell progresses to the G2?
Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.
Define Regulation of intracellular Ca concentration? The cytoplasmic calcium concentration is regulated by a series of calcium pumps, which either concentrate calcium ions with
Pathophysiology Distension and damage of the respiratory bronchioles selectively occur. Opening develops in the walls of the bronchioles. and they become enlarged and conf
Define Methods of Quantitative Analysis and Colorimetry? Many methods of quantitative analysis are based upon the production of coloured solutions in such a way that the intens
Meningococcal disease Meningococcal vaccine is recommended for adults and children >2 years old who are traveling to areas where epidemics are occurring, or to the "meningitis
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Neuropsychological assessmnet of older children In the recent years, the field of child neuropsychology has undergone tremendous growth. There has been a burgeoning interest in
What is Lysosomes? Lysosomes : Animal and fungal cells contain membrane-bound organelles called lysosomes, which are filled with digestive enzymes. These digestive enzyme
a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample
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