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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain the Solid-Liquid Equilibria? A freezing-point curve (freezing point as a function of liquid composition) and a solubility curve (composition of a solution in equilibriu
Explain the NATURAL HISTORY OF CORONARY ARTERY DISEASES (GAD)? The natural history of CAD is very important from the preventive point or view. Though the usual manifestations o
Cytoplasmic bridge formed during the conjugation of paramoec: The two paramoecia that undergo conjugation are called conjugants. During conjugation, the two conjugants com
Q. What is tubal pregnancy? Many times fecundation takes place in the Fallopian tubes in general the newly formed zygote is taken to the uterus where nidation and the embryonic
Simple diffusion Only comparatively small uncharged or hydrophobic molecules (H2O, O2, and CO2, other gases, urea and ethanol) cross the lipid bilayer through simple
What are the typical components of a closed circulatory system? The typical components of the closed circulatory system are the blood vessels within which blood circulates (vei
Why does the recombination frequency of genes vary with the distance between them in the chromosome? The farther the distance among the loci of two genes in a chromosome the h
Animal tissue
How did muir's impression of man's relationship with nature differ from Susan Fenimore coopers? And How did Gifford Pinchot view nature?
Life cycle of Filarial worm Filariasis : It is popularly known as elephantiasis. It is caused by a nematode parasite, wuchereria bancrofti, and W.Malayi. Parasite : Wucher
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