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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain in details Aerobic Respiration? The pyruvate produced in glycolysis is further broken down in most organisms. This process, called aerobic respiration, requires gaseou
Q. List five advantages of DSME? Following are the advantages of DSME. It enables the patient to 1. Accept the disease. 2. Gain knowledge about disease, its prevention
To make a 40 mg/mL solution of kanamycin (MW 582.6) how many grams of kanamycine would you dissolve in 500 mL of water?
Q. How does the poison cyanide act upon the aerobic respiration? Cyanide is a poison that restrains the last cytochrome of the respiratory chain, interrupting the ATP formation
A classic study of extrasensory perception tests the ability of one person to read another's mind. The test uses five cards with different designs, all known to both participants.
Special type of Wards: Additional accommodation or differences in size and shape of some of the special type of wards e.g. Intensive Care Ward, Infectious diseases ward and
What are the functions of the spleen? Why is a total splenectomy (surgical removal of the spleen) compatible with life? The spleen has many functions: it participates in the de
Types of overpopulation - Consumption overpopulation Consumption overpopulation is a phenomenon seen in technologically advanced and affluent countries such as U.S.A., Japan,
Among the different natural hazards, earthquakes happen to be the most feared and dangerous because of their sudden impact and the devastation they cause in a matter of few seconds
What is the Speech Perception Test The subject is asked to listen to a series of 60 sounds, each of which consists of a double e digraph with varying prefixes and suffixes. The
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