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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Define role of Vitamin K in controlling gene expression? Prithrombin, a protein required for blood clotting is assembled with a large number of glutamic acid residues. In the p
The client was admitted to the hospital with hypertension (high blood pressure). The development of arteriosclerosis (plaque buildup in the arteries) has increased peripheral resis
Give three examples of diseases caused by bacteria, Diseases caused by bacteria contain tonsillitis, blood poisoning, scarlet fever, tuberculosis, typhoid, diphtheria, food poi
the Mendel's first law? A Mendel's first law postulates that a characteristic (trait) of an individual is always determined by two factors, one inherited from the mother and th
Implant Mobility and Discomfort Implant mobility is an indication of the lack of osseointegration. Even if periimplant disease has progressed relatively far, implants may still
differences regarding Tiger Planaria and the Iridescent Phyllodoce
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Explain Research dietitians Research dietitians work in the field of normal or therapeutic nutrition Research dietitians seek ways to improve the nutrition of both healthy
Q. What are Diastolic Compressive Forces? Coronary perfusion pressure equal to pressure gradient between the coronary arteries and the pressure in LV in diastole. When coronary
Tricks of learn a classification (molusca,arthopoda,prifera etc.)
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