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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Hormones Controlling Other Functions Crustacean's exhibit pronounced capacity for physiological color changes. It is known that the color changing mechanisms in Crustacea are
Explain Indications for Surgery of Total Anomalous pulmonary Venous Connection ? Once TAPVC is diagnosed in a neonate with symptoms, operation should be undertaken immediately.
Q. How DNA-RNA hybridization occurs Both DNA and RNA are able to form hybrids in solution with other RNA or DNA molecules which have complementary base pairing. Double-stranded
(a) Name the condition and (b) describe the effects of the failure of the pancreas to produce sufficient-insulin. (c) How is this condition treated? .
how do they adapt to their parasitic mode of feeding
Suppuration High number of PMN cells have been detected around implants that are associated with severe signs of mucosal inflammation. Several studies have shown the presence o
Importance of water in the body Water is the most essential constituent of life. Life cannot exist without water. About 90% of water is present in protoplasm Water
what are the role of microbes in human welfare
Q. Treatment and Management of Hypertension? The first choice of treatment and management of primary hypertension is through behaviour modifications pertaining to food choices
The F2 phenotypic ratio from a dihybrid cross with epistasis was 9:3:4. If instead of a dihybrid cross, a double heterozygote was test crossed (crossed to a homozygous recessive in
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