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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
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In a population of 200, 90 are homozygous dominant, 40 are heterozygotes, and 70 are homozygous recessive for a particular trait. What do you expect the frequencies of the dominant
Describe what happens during activation of the lac operon. Lactose binds to the repressor protein, which causes the repressor protein to be released from the operator site. Th
Define Procedure for Standardization of HCl for Saponification Number of Fats? Precisely weigh 2.65 g of anhydrous sodium carbonate and transfer it to 100 ml volumetric flask.
In his experiments, Mendel noted that when two traits are involved in a genetic cross, they are inherited independently of each other. The reason for this is that A. genes on the s
extracellular signals and their characteristics
how trematodes/nematodes adapt to their parasitic mode of feeding
Define Body Building functions of proteins? The primary functions of proteins, as you might be aware, is tissue growth and maintenance. Protein contains amino acids - the build
Q. Which are mineral salts? Where in living beings can found mineral salts? Ans. Mineral salts are simple inorganic substances made of metallic chemical elements, like sodiu
Discuss in detail about the Close Head Injuries Closed-head injuries result from a blow to the head, which can subject the brain to a variety of mechanical forces: Damag
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