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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Explain Adverse Effects Fluconazole is generally well tolerated. Gastrointestinal distress and rash can happen. Stevens-Johnson syndrome, anaphylaxis and hepatic necrosis have
When the standard free energy of a reaction is -5.7 kj/mol then at equilibrium a- there will be 10 times more products than reactants b-there will be 10000 times more product
Q. What is the physiological cause of the syndrome Called as cretinism? Cretinism is caused by chronic deficiency of the thyroid hormones (T4and T3) during childhood. The chron
Study of tonana larva Tornaria larva is typical in the life-history of hemichordates. Examine the permanent slide and note the following features: I. The tornaria larva usu
Explain the Determination of Hydrolysis Hydrolysis of calcium pantothenate in acidic medium produces α,γ-dihydroxy-β,β-dimethylbutyrolactone, which reacts with hydroxylamine i
Cellular respiration is the transfer of energy from various molecules to produce ATP; takes place in the mitochondria of the eukaryotes, the cytoplasm of prokaryotes. In the proce
Hypertension is one of the commonest disease states encountered in medical practice. It is one of the simplest to diagnose and to treat if one follows certain guidelines which are
Diagnostic Evaluation A study of complete blood count (CBC) and blood chemistry mainly uric acid levels, is necessary for dignostic evaluation. Radiologic studies are
Why Iodine is Important for Human Body? Iodine is an essential constituent of the thyroid hormones: thyroxine (T 4 ) and triiodothyronine (T 3 ), which have a key role in growt
What is an etiological agent of disease? An etiological agent of disease is the agent that causes the disease. It might be a living being, substance or environmental fact.
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