Expression clone, Biology

Assignment Help:

Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.


Related Discussions:- Expression clone

How many chromosomes and dna molecules found per cell, A cell in G1 of inte...

A cell in G1 of interphase has 12 chromosomes. How many chromosomes and DNA molecules will be found per cell when this original cell progresses to the G2?

Define dietary management of cancer patients, Define Dietary Management of ...

Define Dietary Management of Cancer Patients and Feeding Problems Related To Cancer Therapy? We took an overview about the general nutritional requirements of cancer patients.

Define regulation of intracellular ca concentration, Define Regulation of i...

Define Regulation of intracellular Ca concentration? The cytoplasmic calcium concentration is regulated by a series of calcium pumps, which either concentrate calcium ions with

Signs and symptoms of emphysema, Pathophysiology Distension and damage...

Pathophysiology Distension and damage of the respiratory bronchioles selectively occur. Opening develops in the walls of the bronchioles. and they become enlarged and conf

Define methods of quantitative analysis and colorimetry, Define Methods of ...

Define Methods of Quantitative Analysis and Colorimetry? Many methods of quantitative analysis are based upon the production of coloured solutions in such a way that the intens

Explain meningococcal disease, Meningococcal disease  Meningococcal vac...

Meningococcal disease  Meningococcal vaccine is recommended for adults and children >2 years old who are traveling to areas where epidemics are occurring, or to the "meningitis

Estrogens, Normal 0 false false false EN-IN X-NONE ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Neuropsychological assessmnet of older children, Neuropsychological assessm...

Neuropsychological assessmnet of older children In the recent years, the field of child neuropsychology has undergone tremendous growth. There has been a burgeoning interest in

What is lysosomes, What is Lysosomes? Lysosomes :  Animal and fungal ...

What is Lysosomes? Lysosomes :  Animal and fungal cells contain membrane-bound organelles called lysosomes, which are filled with digestive enzymes. These digestive enzyme

Original density in CFU/ML, a nutrient agar plate labelled 10^(-5)ml had 15...

a nutrient agar plate labelled 10^(-5)ml had 154 colonies after incubated. what is the cell density in the original sample

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd