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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
Fate of the germ layers In cnidarians and ctenophores, all the cell types develop from either ectoderm or endoderm. Similarly all the tissues and organs in the rest of the ani
Principle for Estimation of Percentage of Reducing and Total Sugar by Fehling Soxhlet Method? Reducing sugars are those which have free sugar groups and hence may be estimated
Q. What are the three kinds of protein fibers of the connective tissue proper? The matrix of the connective tissue proper is made of elastic fibers, collagen fibers and reticul
Advantages and disadvantages of protozoa
Anti Platelet Drugs In the early years of CABG, patients used to be put on aspirin and persantin (Dipyridamole). Persantin is not routinely prescribed now. Aspirin dose can b
Importance of Dark Period For quite sometime the role of light period (photoperiod) was emphasised in flowering? However, based on certain experiments it was realised that it
What are the lowest and highest temperatures humans can survive? Being in cold temperature air with proper shelter and clothing, humans can endure indefinitely, even if the tem
Where do the photochemical and the chemical stages of photosynthesis occur? The photochemical stage of the photosynthesis process happens mainly on the thylakoids (the green pa
ARTERIAL SYSTEM OF MAMMAL Basic plan from origin to distribution is similar in all vertebrates as per the mode of respiration. Main anterior aorta in terrestrial forms ar
translation in eukaryotes..
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