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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A VERY SIMPLE WAY.
main external morphological feature that differentiates platyhelminthes from nematodes
Why can it be said that each glucose molecule runs the Krebs cycle twice? Every glucose molecule "cycles" the Krebs cycle twice because after glycolysis each used glucose has g
How many times more acidic is a pH=4 from a pH=7 solution?
Explain about the Reduction Tests - Carbohydrates? These are a group of tests answered by reducing sugars. Since we have already discussed reducing sugars, you will be able to
Kinds of Ovules Ovules undergo varied degrees of curvature during development. The position of micropyle with respect to the funiculus becomes different and constitutes the b
Discuss mental nerve, its anatomic variation and surgical importance. Mental nerve, branch of inferior alveolar nerve is a very important landmark during osteotomy procedures.
what is common between aquatic and terrestrial plants
Why Fish and Sea Foods are important for human Body? They are rich sources of proteins (20-25%) of a high biological value. Dry fishes contain more (60%) proteins since most of
ARTERIAL SYSTEM OF MAMMAL Basic plan from origin to distribution is similar in all vertebrates as per the mode of respiration. Main anterior aorta in terrestrial forms ar
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