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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
hpw many neurons do we have?
It is mostly spread by sexual contact, blood transfers and from HIV infected mother to child.
Other agro-industrial byproducts Supply of nutrients in the livestock ration can be maintained by using the locally available industrial byproducts, which in spite of having h
Renal insufficiency Antimicrobial drugs excreted through the urinary tract may be toxic for patients with renal insufficiency if they are given in usual therapeutic doses, bec
Q. What are the Causes of constipation? The most common causes of constipation are poor elimination habits, a lack of fibre in the diet, insufficient fluid intake, lack of exer
parasitic adaptation of round worm
A plant grown from one of Mendel's yellow pea is selfed. Three progeny peas from this are obtained and they are all green. Is the original plant homozygous or heterozygous, and wha
WHAT IS SYMBIOTIC THEORY. PLEASE EXPLAIN THIS TO ME IN A VERY SIMPLE WAY.
Q. Show the Paleobotanical Evidence? Paleobotany deals with the study of fossil records of plants and animals. New techniques and approaches to the study of fossil flowering pl
explanation of examples of protozoa
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