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Expression clone is a clone (plasmid in the bacteria, or might be a lambda phage in bacteria) which is designed to produce the protein from the DNA insert. Mammalian genes do not function properly in bacteria, so to get bacterial expression from your mammalian cDNA, you would need to place its coding region (that is no introns) immediately adjacent to bacterial transcription or translation control sequences. That artificial construct (which is "expression clone") will produce the pseudo-mammalian protein if put back into bacteria again. Often, that protein can be recognized by the antibodies raised against the authentic mammalian protein, and vice versa.
What are some industrial processes that use bacteria? Bacteria are used by industry in many ways. There are vaccines made of attenuated pathogenic bacteria or of antigens prese
In the human body, the potassium ion can pass easily through cell membranes, yet the potassium ion concentration is higher inside many cells than it is outside these cells. Could t
The different organs of the respiratory system Nostrils through which we inhale air in the nasal cavity Pharynx Larynx Trachea (wind pipe) Lungs - Bronchi,
Given four respiratory capacities (a - d) and four jumbled respiratory volumes of a normal human adult: Respiratory Respiratory capacities volumes. 1. Residual vol
Which of the following are bound to hemoglobin when hemoglobin is in the R-state? Choose all that apply. 1. Fe2+ 2. CO2 3. 2,3-bisphosphoglycerate 4. Fe3+ 5. Oxygen
There are several different lipoprotein species found in plasma but their basic structures are similar. The insoluble lipid (cholesterol ester and triglyceride) forms a central cor
Q. Can two normal individuals of the same species with sexual reproduction have identical karyotypes and identical genomes? And how is the human karyotype usually represented?
Which of the below is usually referred to as "master gland" because it regulates so many body activities? Is it: a) Pituitary gland b) Thyroid gland c) Adrenal gland d
How are the symmetry and the nervous system characterized in echinoderms? Adult echinoderms, with cnidarians, are the animals that present radial symmetry, i.e., their body str
Q. What are the elements that form the middle ear? What are the names of the three middle ear ossicles that participate in the phonosensitivity? The middle ear is formed by the
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