Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Serial Dilution Process - Plate Counts?
Dilution factor is the reciprocal of the dilution used. As number of microorganisms in original sample is not known, the original sample is diluted several times in sterile water blanks to ensure that separate colonies are developed and some colony counts will be in the specified range. The process is called serial dilution. The procedure is shown in Figure. Usually a ten-fold serial dilution is used in the process where 1 ml of the sample is transferred to a 9 ml of the diluent to get 1:10 dilution. 1 ml of this is transferred to another tube of 9 ml of diluent to get 1:100 dilution and so on till the required dilutions are obtained.
Figure: Serial dilution process
It is important that the dilution used should produce a limited number of colonies on plate. When many cells are present, overcrowding of cells may not allow many colonies to develop and may also result in many colonies to fuse. This leads to inaccuracies in count. Similarly, too less colonies are not statistically significant. According to Food and Drug Administration, only those plates having 25-250 colonies should be considered for calculation; but most microbiologists use plates with 30-300 colonies to get the microbial numbers in the sample. Plates getting colonies less than 30 are marked too less to count (TLTC) and getting more than 300 are marked as too numerous to count (TNTC) and are discarded.
What are the functions of the spleen? Why is a total splenectomy (surgical removal of the spleen) compatible with life? The spleen has many functions: it participates in the de
PURPOSES OF ASSESSING GROWTH AND DEVELOPMENT IN CHILDREN Before you assess the growth and development you should be aware of the purpose of monitoring. The purposes are a
What are plant tissues that constitute the functional structures of the leaf veins ? The Leaf veins are made of the vascular tissues.They are constituted by the xylem and the p
Define Determinants of Food Security - Food Availability? It depends on the quantum and quality of crops, livestock, fishery and other food sources, as well as commercial impor
information on climbing beans
Plants are supplied with adequate amount of phosphorus Phosphorus has also been associated with early maturity of crops particularly the cereals. Its shortage is accompanied by
ae there non-parasitic viruses?
HIV (Human Immuno Deficiency Virus) Infection and AIDS (Acquired Immune Deficiency Syndrome)
Define nutritional management of severe anorexia nervosa? The nutritional management of severe anorexia nervosa is therefore, considered in terms of three consecutive phases:
It is said during puberty rising sex hormones stimulate osteoblasts to produce bone faster than the rate at which epiphyseal cartilage expands. The timing of epiphyseal closure dif
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd