Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Explain the Serial Dilution Process - Plate Counts?
Dilution factor is the reciprocal of the dilution used. As number of microorganisms in original sample is not known, the original sample is diluted several times in sterile water blanks to ensure that separate colonies are developed and some colony counts will be in the specified range. The process is called serial dilution. The procedure is shown in Figure. Usually a ten-fold serial dilution is used in the process where 1 ml of the sample is transferred to a 9 ml of the diluent to get 1:10 dilution. 1 ml of this is transferred to another tube of 9 ml of diluent to get 1:100 dilution and so on till the required dilutions are obtained.
Figure: Serial dilution process
It is important that the dilution used should produce a limited number of colonies on plate. When many cells are present, overcrowding of cells may not allow many colonies to develop and may also result in many colonies to fuse. This leads to inaccuracies in count. Similarly, too less colonies are not statistically significant. According to Food and Drug Administration, only those plates having 25-250 colonies should be considered for calculation; but most microbiologists use plates with 30-300 colonies to get the microbial numbers in the sample. Plates getting colonies less than 30 are marked too less to count (TLTC) and getting more than 300 are marked as too numerous to count (TNTC) and are discarded.
Q. What is the salivary digestive enzyme? Which type of food does it digest and into which smaller molecules does it transform the food? The salivary hydrolase is known as sali
what is the chemical reaction of respiration in cockroach?
Among neonates, IE typically involves the tricuspid valve of structurally normal hearts and is associated with very high mortality rates. It is likely that many of these episodes
LARYN X - Study of larynx is known as Laryngeology. In man it is more prominent than woman known as Adam's apple. It is sound producing organ. At its base trachea presen
What is the difference between xylem and phloem? Both xylem and phloem are vascular tissues found in a plant. Xylem is a tubular structure, which is liable for water transport
Allosteric enzymes are those that have more activation center and to which other substances known as allosteric regulators bind. Allosteric regulators can be allosteric inhibito
biology ofr this
. which is the main part of respiratory organ?
What is light reaction? Light reactions are reactions, which are initiated by light (also photo-induced reactions). Usually, light energy is change into chemical energy.
Is gene a triplet of consecutive DNA nucleotides A gene isn't a triplet of DNA nucleotides with their respective nitrogen-containing bases, like CGT or AAG. The nucleotide trip
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd