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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
Explain the Competitive behaviour of social animal group dominance behaviours removal of individuals to other groups which minimises inbreeding eg lions, apes.
type study of polystomella
Cereal and Gram By-Products Agro-processing is now regarded as the sun rise sector in view of its large potential in maintaining the nutritional quality of processed feed resource
Which of the following defines why the vast majority of segregation errors in human patients include the two sex chromosomes (XXY, XO, XYY) or chromosome 21 (triple 21)? A. Th
Habits of patients- Implants Smoking has been shown to predispose to poor bone quality by reduced vascularity. Therefore it is prudent not to accept heavy smokers as implant pa
Q. What is the glycolysis? And what are the products of this process? Glycolysis, the first stage of the aerobic cell respiration is a process in which glucose is degraded brok
PITUITARY DISORDERS - (a) Pituitary Dwarfism . It is caused by the deficiency of growth hormones (GH) from childhood. It is characterised by small but well porportioned body a
When underlying coronary artery disease is the cause of heart failure in the coronary revascularization may both improve symptoms and prevent progression. Patients with angina and
Does every amino acid have a central carbon? To which organic group is that central carbon bound? A carboxyl group -COOH, an amine group - NH 2 , an atom of hydrogen -H and a v
Explain Transfer of Culture from One Liquid Media to another Liquid Media? 1. Inoculating loop is to be held in the right hand. It is sterilized by incineration in the inner bl
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