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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
Rhinosporidiosis (Fungal-like agent) Rhinosporidiosis is a chronic, non-fatal, granulomatous mycotic infection of nasal mucosa caused by Rhinosporidium seberi. R. seberi is curren
Planning the Nursing Care The goals of nursing care are: Identify the causative factor of megaloblastic anaemia. Administer appropriate vitamins depending on
In this section we are to discuss the means by which populations get separated or isolated from each other, first gain the status of'Sub-species and finally evolve mechanisms which
PECTIN It consists of methylated galacturonic acid, galactose & arabinose. It is also called plant cement becuase it binds layers of cell wall. It is a structural poly
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In the presence of oxygen, the process of glycolysis produces which of the following products? A) 2 glycerol B) 1 lactate C) 2 lactate D) 2 pyruvate E) 1 pyruvate
Q. What is the official name of pyruvic acid? Pyruvic acid is named as 2-oxopropanoic acid it is thus a molecule made of three linearly bound carbons with one extremity forming
Determine the net charge of the predominant form Asp at (a) pH 1.0, (b) pH 3.0, (c) pH 6.0, and (d) pH 11.0?
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