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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
How is excretion done in fishes? Fishes have a pair of kidneys that filtrate the blood. Bony fishes excrete nitrogen as ammonia, NH 3 , (they are ammoniotelic) and cartilagino
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Q. Fluoxetine is an antidepressant drug that presents an action mechanism related to the synaptic transmission. What is that mechanism? Fluoxetine is a substance that inhibits
Explain the Position of the endospore? Endospore cannot be stained by ordinary methods such as simple staining and gram staining because these dyes do not penetrate the wall of
Classify phylum Aschelminthes
Explain the Management of Furcal Perforation 1 st isolate the perforation site. If the perforation is mechanical accidentally occurred: a- It is probably
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Raised blood pressure is a major risk factor for cardiovascular disease. The higher the blood pressure, the higher the risk of stroke, coronary heart disease, kidney disease, heart
What are the common changes in the eye Other changes in the eye are: 1) Increase in curvature of anterior surface of lens. 2) Anterior pole of lens moves forwards without
Explain the Process of dna technology Procedures involving recombinant DNA technology has been used to increase the yield of enzymes already produced by the microorganisms, e.
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