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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
Explain Procedure for Isolation of Pure Culture? Now carry out the exercise following the steps enumerated herewith. 1. Label the name of the organism on the bottom of the p
RECIPROCAL CROSSES Similar results were obtained with reciprocal crosses also. A reciprocal crosses involves the same traits but carried by sexes opposite to those in the origi
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different types of respiration system in different organisms
Q. How is carbon dioxide made by producers and consumers? The Carbon dioxide is made by consumers and producers through cellular respiration.
Examples of trinomial nomenclature
Explain Nutritional Biochemistry A good understanding of the biochemical basis of nutrient function and of the consequence of nutrient deficiency or excess is impo
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Explain Objectives of Root-End Resection Apicoectomy a) Removal of untreated apical portion of the root, b) Enable the obturator to determine the cause of failure, c) To
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