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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
In vitro Studies - Nutrition of Embryo Studies involving culture of embryos excised of various stages of development (i.e., early globular proembryo, late globular proembryo o
VERTEBRAL COLUMN - It is curved vertical about 70 cms. long. It consists of 33 vertebrae (after fusion 26). It shows 4 curvatures - (i) Cervical curvatures
Mice (Mus musculus) have 40 chromosomes per diploid cell (2n=40). How many double- stranded DNA molecules, and how many chromosomes are there in a mouse cell that is in the G2 stag
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In the Miller-Urey experiment, amino acids of bothe the L and D enantionmers. Why is it that the L enantionmer is more dominant in living things?
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The bonding geometry of C,O,N is determined by- Select one: a. The number of electrons in their outer energy level b. SP3 hybridization of orbitals in the valence shell
describe the discovery of new fossil evidence for evolution
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