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Explain the Presumptive Test - Most Probable Number Test?
The Presumptive test is used to detect and estimate coliform population of a water sample. This test is carried out by inoculating a series of lactose broth medium with three different sample volumes (10 ml, 1 ml and 0.1 ml). The series consists of 3 groups, each composed of 5 tubes and each group is inoculated with the designated volume of the water sample. The greater the number of tubes per group, the greater the sensitivity of the test. Lactose medium contains the lactose, bile salt and pH indicator.
Lactose and bile salts are selective for coliforms as lactose is used only by coliforms and not by other enteric bacteria while bile salts are depressent for enteric bacteria other than coliforms. A pH indicator such as bromocresol purple is added to the medium to detect acid fermentation from lactose. Production of acid changes the colour of the medium to yellow. Gas production during fermentation is detected by inverted Durham tube in lactose medium. Positive test for coliforms is indicated by the production of acid and gas from lactose fermentation after 24-48 hours of incubation. Number of tubes showing positive test is counted and the result is expressed as most probable number (MPN) of coliforms by matching the results
Promotion - Stages of carcinogenesis The second stage or promotion is reversible and does not include changes in structure of DNA but rather in the expression of the genome of
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Polynephritis in cattle Contagious bovine polynephritis is a specific infection of urinary tract of cattle. The causative agent is Corynebacterium renale, a rod shaped gram-po
recessive epistasis
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Better coal should have following characteristics (1) It should have high calorific value. (2) It should have low moisture content. (3) It should have low ash conte
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