Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Hollow restorations for re-lining, A hollow restoration may be fabricated b...

A hollow restoration may be fabricated based on the original form determined by the diagnostic preview. This restoration can be re-lined on completion of the surgical phase of impl

Difference between a bird heart and a mammalian heart, What is the main dif...

What is the main difference between a bird heart and a mammalian heart? The bird heart has a one aortic arch on the right side of its body while the mammalian heart has one on

Drug effects on food intake - cause sore or painful mouth, Define Drug effe...

Define Drug effects on food intake - Cause sore or painful Mouth? This is a common side effect of chemotherapy and can significantly affect food intake. Antineoplastic drugs, u

Could you survive on a diet which contained no carbohydrate, Could you surv...

Could you survive on a diet which contained no carbohydrate?   It should be possible to survive without carbohydrate as energy can be obtained from fats and proteins.

Chordal sparing operation- mitral valve replacement, Chordal Sparing ...

Chordal Sparing Operation : For sparing both leaflets, an incision is made on the anterior leaflet close to the annulus and then extended centrally and the two segments,

Assume the effect on turnover, Explain two ways in which a changing climate...

Explain two ways in which a changing climate may impact the nutrient cycle. Predict  the effect on turnover.

Microbiology, How to use microorganisms to control diseases

How to use microorganisms to control diseases

Calculate the ph - microbial survival and growth, Calculate the pH - microb...

Calculate the pH - microbial survival and growth? Presence of microorganisms also depends on the pH of the medium. Most bacteria grow optimally near neutral pH (optimum 6.5 - 7

Nutrition - heterotrophic nutrition, HETEROTROPHI C NUTRITION Animals ...

HETEROTROPHI C NUTRITION Animals derive organic food materials by consuming products of plants - Heterotrophic nutrition. T ype s of Heterotrophic Nutrition (i ) H

What is biotic potential, Q. What is biotic potential? The Biotic poten...

Q. What is biotic potential? The Biotic potential is the capability of growth of a given population under hypothetical optimum conditions, i.e., in an environment without limit

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd