Explain the indole production test, Biology

Assignment Help:

Explain the Indole Production Test?

Many microorganisms produce an enzyme tryptophanase which oxidizes amino acid tryptophan to produce pyruvic acid, indole and ammonia. We can analyze the production of tryptophanase by using SIM agar containing tryptophan. The presence of indole is then detected by adding Kovac's reagent which contains p-dimethyl amino benzaldehyde, butanol and hydrochloric acid. It produces a cherry-red reagent layer with indole (positive test).The role of acidified butyl alcohol is to extract the indole into the reagent layer which then react with dimethyl amino benzaldehyde.


Related Discussions:- Explain the indole production test

Respiratry system, prepare a power point presantation of respiratry system

prepare a power point presantation of respiratry system

Regulation of blood glucose level, REGULATION OF BLOOD GLUCOSE LEVEL Va...

REGULATION OF BLOOD GLUCOSE LEVEL Various levels of regulation are exerted at substrate level, hormonal level, enzymatic level and at organ level on carbohydrate  metabolism  s

What is salmonella typhosn, What is salmonella typhosn Typhoid is  an e...

What is salmonella typhosn Typhoid is  an enteric fever, which relates  to acute infection of  short duration. It is   caused by bacteria called Salmonella typhosn about which

What is class gastropoda in animal kingdom, What is Class Gastropoda in ani...

What is Class Gastropoda in animal kingdom? Snails and slugs are referred to as gastropods because of their characteristic body plan. "Gastro" is derived from the Greek word fo

Precautions for determination of fungal and yeast count, Explain Precaution...

Explain Precautions for Determination of Fungal and Yeast Count? 1. Maintain aseptic conditions. 2. Keep alcohol away from flame. 3. To avoid infections and allergies, ca

Which is structural isomers, Structural isomers: Select one: a. Have ...

Structural isomers: Select one: a. Have the same molecular weight b. Have the same connectivity c. Are mirror images d. All of the above e. None of the above

Can you describe about mechanical digestion, Q. What is mechanical digestio...

Q. What is mechanical digestion? In molluscs, earthworms, arthropods, birds and vertebrates, in general, which organs respectively participate in this type of digestion? Mechan

What is the function of the myelin sheath, What is the function of the myel...

What is the function of the myelin sheath? Do all axons present a myelin sheath? The function of the myelin sheath is to get better the safety and speed of the neural impulse t

Composition of cell wall, COMPOSITION OF CELL WALL Cell wall composed ...

COMPOSITION OF CELL WALL Cell wall composed of cellulose, hemicellulose, pectin, lignin, cutin & chitin. Cell wall consists of 3 parts - 1. Matrix Highly cross-

Foraminifera, Foraminifera is the single-celled protists which secrete a s...

Foraminifera is the single-celled protists which secrete a shell or test. This is the accumulations of the shells of dead foraminifera and the other microscopic sea creatures form

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd