Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

What is the destination of the branchial clefts in humans, What is the dest...

What is the destination of the branchial clefts in humans? In humans the branchial clefts situated in the anterior region of the pharynx (also called as pharyngeal clefts) are

Total darkness and pea seedlings grown in the light, What differences would...

What differences would you expect to see among pea seedlings grown for 10 days in total darkness and pea seedlings grown in the light for the similar period of time?   The s

Chlorofluorocarbons cause global problem, In what way do chlorofluorocarbon...

In what way do chlorofluorocarbons (CFCs) cause global problems? Chlorofluorocarbons react with and deplete the ozone in the ozone layer. This permits more ultra-violet light f

Nervous system control of blood pressure, Q. Nervous System control of bloo...

Q. Nervous System control of blood pressure? Most nervous controls are achieved via involuntary reflex arcs involving pressoreceptors, the vasomotor centers of the medulla, and

Zoo201, In an outline form only, attempt the classification of the phylum a...

In an outline form only, attempt the classification of the phylum arthropoda

How does hypophysis- corpus luteum negative feedback work, Q. How does the ...

Q. How does the hypophysis- corpus luteum negative feedback work? What is the name given to the atrophied corpus luteum after this feedback process? After ovulation the estroge

National health service systems, Normal 0 false false false...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

In hormonal terms why does menses occur, In hormonal terms why does menses ...

In hormonal terms why does menses occur? Menses is the endometrial monthly desquamation that happens as the estrogen and progesterone levels fall after the regression of the co

Stereoisomers , Glyceraldehyde has a one asymmetric carbon atom the central...

Glyceraldehyde has a one asymmetric carbon atom the central one and so two stereoisomers  which is also known as optical isomers are possible, which is two forms of glyceraldehyde,

Urea molasses mineral block (ummb) licks, Urea molasses mineral block (UMMB...

Urea molasses mineral block (UMMB) licks Development of urea molasses mineral block licks is another technology being increasingly used in several parts of India and such lick

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd