Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

Health education to hospitalised child, Health Education to Hospitalised C...

Health Education to Hospitalised Child You have unique opportunity for teaching and reinforcing good hygienic care while the child is hospitalised. Clean hair, nails, clothin

How does the inflammation mechanism work, Q. How does the inflammation mech...

Q. How does the inflammation mechanism work? When some tissue injury other vasoactive and occurs histamine substances (called mediators of inflammation) are released, they caus

How are reptilia characterized, Q. Class Reptilia identity card. How are th...

Q. Class Reptilia identity card. How are they characterized according to examples of representing beings, basic morphology, skin, respiration, circulation, nitrogen waste, thermal

Define Stolon - Types of hyphae, Define Stolon - Types of Hyphae? Mic...

Define Stolon - Types of Hyphae? Microscopically, hyphae are aseptate and coenocytic. There are 3 kinds of hyphae: (a) Stolon - These grow horizontally on substratum surfa

What is aneuploidy, What is aneuploidy? What are the conditions caused by t...

What is aneuploidy? What are the conditions caused by the aneuploidies? The Aneuploidy is an abnormal number of chromosomes in the cells of an individual. The major aneuploi

Viral diseases - avian encephalomyelitis, V i ra l Diseases A v ...

V i ra l Diseases A v ian encephalomyelitis This is enterovirus disease of chicken, pheasants, turkeys and Japanese quails caused by the member of the family Pi

Genetics what is this type of gene frequency change called, An hypothesis f...

An hypothesis for the extinction of the dinosaurs is that the earth had been hit by a gigantic meteor that caused the death of those big reptiles. In that case the entire genetic p

Explain the role of calcium ions and tropnin, Explain the role of calcium i...

Explain the role of calcium ions, troponin and F-Actin during contraction in striated muscles of humans?

Define the nutritional and functional role of nickel, Minerals :-Nickel  ...

Minerals :-Nickel  Food Source      Plant foods  Nutritional Functional role Essential nutrient: Deficiency in humans unknown. Catalyst: hydrogenation in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd