Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

Growth and development, what is the clear explanation of growth and develop...

what is the clear explanation of growth and development

Safety, S a f e ty, ethical and welfare considerations Development ...

S a f e ty, ethical and welfare considerations Development of transgenic organisms is proceeding in an environment of intense public debate about safety, environmental impa

Are sex-linked diseases associated only to genes, Are sex-linked diseases a...

Are sex-linked diseases associated only to genes of the X chromosome? There are many X-linked diseases, like hemophilia A, hemophilia B and adrenoleukodystrophy, but called as

Name the restricted food items, Restricted Food Items Foods to be avoid...

Restricted Food Items Foods to be avoided by diabetic patient are carbohydrate and fatty food. Carbohydrates are classified into sugars and starches. As you have studied in und

How are cnidarian characterized, Q. Cnidarian identity card. How are they c...

Q. Cnidarian identity card. How are they characterized according to instance of representing beings, basic morphology, kind of symmetry, germ layers and coelom, digestive system, r

Polyphyletic theory, Please I need short notes on polyphylrtic theory

Please I need short notes on polyphylrtic theory

Discuss about the halstead reitan battery, Discuss about the Halstead Reita...

Discuss about the Halstead Reitan battery The Halstead Reitan battery continues to be widely used as a clinical and research procedure. Numerous investigators use it in their r

Explain the complications of burns, Explain the Complications of Burns? ...

Explain the Complications of Burns? Most minor burns are superficial and do not cause complications. However, deep second-degree and third-degree burns swell and take more time

Which terrestrial vertebrate group is extremely rare, Which terrestrial ver...

Which terrestrial vertebrate group is extremely rare in deserts? Amphibians are terrestrial vertebrates extremely rare in desert environments (although there are a some species

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd