Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

Functions of the vascular system, Functions of the Vascular System The...

Functions of the Vascular System The major function of the circulatory system is to provide a transport mechanism for the blood flow to and from the heart. The vascular syste

Explain the coliforms - microbiological study of water, Explain the Colifor...

Explain the Coliforms - Microbiological Study of Water? These are widely used indicators which belongs to the family Enterobacteriaceae and make up about 10% of the intestinal

What are organelles, What are organelles, which carry out autophagy? Au...

What are organelles, which carry out autophagy? Autophagy, being the degradation of cytoplasmic components as well as other organelles, is usually done with the lysosomes. The

Techniques of plant tissue culture, Techniques of Plant Tissue Culture ...

Techniques of Plant Tissue Culture A standard tissue culture laboratory should provide facilities for washing and storage of glass ware, preparation and storage of nutrient

Explain salient features of pulmonary tuberculosis, Salient Features of pul...

Salient Features of pulmonary tuberculosis The salient features tuberculosis include: Wasting of tissues Exhaustion Cough Expectoration, and Fever The acute p

Explain directive and non-directive counseling, Directive and Non-directive...

Directive and Non-directive Counseling Directive Counseling  tends  to  be appropriate when  the  counselor  is  aware  of  the problem  and/or  is concerned about  the behavio

Cartilaginous and the osseous tissue, Q. Which kind of tissue are the carti...

Q. Which kind of tissue are the cartilaginous and the osseous tissue? The osseous and the cartilaginous tissues are considered connective tissues since they are tissues in whic

List principles to keep in mind for designing the messages, Q. List princip...

Q. List principles to keep in mind for designing the messages? o Develop a positive theme. Approaches are more effective when they promote positive messages that state clear

Zoonoses disease- colibacillosis, Colibacillosis Colibacillosis is caused ...

Colibacillosis Colibacillosis is caused by different strains of Escherichia coli. The disease mainly affects children and travelers. Organisms grow in gut and produce toxin. It ma

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd