Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

How does the swim bladder of fishes work, Q. How does the swim bladder of f...

Q. How does the swim bladder of fishes work allowing fishes to control their depth under water? From Hydrostatics it is known that an object does not sink if its density is les

Do echinoderms present internal or external fecundation, Do echinoderms pre...

Do echinoderms present internal or external fecundation? Is there sex division among individuals? The fecundation in echinoderms is external; gametes are liberated in water whe

Agro industrial-complete feed blocks, Complete feed blocks For manufac...

Complete feed blocks For manufacturing the pellets, processes such as grinding, mixing, steaming and pressing and some times extruding, are applied using special and expensive

Explain phylum zooflagellata, Phylum Zooflagellata (Animal Flagellates)...

Phylum Zooflagellata (Animal Flagellates) 1) At some stage of life-cycle they have one or more flagella. 2) They feed heterotrophically by absorption or phagocytosis, and

Give choice of catheters for ventriculography, Q. Give Choice of Catheters ...

Q. Give Choice of Catheters for Ventriculography? 1) Pigtail Catheter: This catheter developed by Judkins has end hole and side holes. The end hole permits insertion of the c

Why are pteridophytes more common in humid places, Q. Why are pteridophytes...

Q. Why are pteridophytes more common in humid places? Pteridophytes are more common in humid places since they depend on water for their gametes to fecundate one another. In hu

Floriculture, Floriculture: This is the cultivation and development of com...

Floriculture: This is the cultivation and development of commercially important floral plants like rose, lilies, etc. Floriculture is called flower farming is a discipline of hort

Dictyosomes, Dictyosomes  are produced on the Organelles in the plant cells...

Dictyosomes  are produced on the Organelles in the plant cells composed of the series of flattened membrane sacs which sort, chemically modify, and package protein srough endoplasm

Restriction endonuclease, Suppose the restriction endonuclease HindIII cuts...

Suppose the restriction endonuclease HindIII cuts a6.0 kb linear piece of DNA into two fragments; an 800 bp fragment and a 5200 bp fragment..... Question: Suppose restriction

Show the collection of primary samples of grain, Q. Show the Collection of ...

Q. Show the Collection of Primary Samples of grain? Sampling of grains begins with the collection of the primary sample i.e. sample of the consignment. The process involved in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd