Explain the principle of microscopy, Biology

Assignment Help:

Explain the Principle of Microscopy?

When the light passes from one medium to another, refraction occurs, i.e., the ray is bent at the interface. The direction and the magnitude of bending are determined by the refractive indices of the two media forming the interface. The refractive index is a measure of how greatly a substance slows the velocity of light when light passes from air to glass or vice versa. When the light rays strike the lens, a convex lens will focus these rays at specific point called focal point. The distance between the center of the lens and the focal point is called the focal length. Convex lens act as a magnifier.

It provides a clear magnifying image at a much closer range. Lens strength is related to focal length. A lens with a short focal length has a more magnification power than a lens having a longer focal length. Magnification means enlargement. In compound microscope, it is carried out by two-lens system - objective lens and ocular lens. Objective lens produces the real image of the specimen, which is projected up into focal plane and then magnified by the ocular lens to produce the final image. Though magnification is important, it has limits. Unlimited enlargement by increasing magnifying power of the lenses is not possible because of the limitations of resolving power. Microscope should not only produce the enlarged image but also the clear image. Resolution or resolving power of the lens is its ability to separate two close points as separate entities.


Related Discussions:- Explain the principle of microscopy

Research and documentation - conservation of wildlife, Research and Documen...

Research and Documentation - Conservation of wildlife First, list of endangered species are established by various national and international agencies. Another important actio

What phenotypic ratios of the diploid, A wild-type strain of haploid yeast ...

A wild-type strain of haploid yeast is crossed to a mutant haploid strain to make a diploid. What phenotypic ratios will be observed in the haploid progeny of the diploid?

Planeria, differnce between protonephridia and metanephridia

differnce between protonephridia and metanephridia

Rehabilitation phase for nutritional treatment -neuro trauma, Rehabilitatio...

Rehabilitation Phase for nutritional treatment - Neuro Trauma? Once the patient stabilizes and starts recovering, be sides nutritional replacement, there is a need to assess fu

How does iodine kill germs, How does iodine kill germs? The microbiocid...

How does iodine kill germs? The microbiocidal action of Iodine is because of the active form, I2, which is polarized by water and like all halogens (chlorine, fluorine, bromine

Explain saquinavir, Explain Saquinavir Saquinavir (SQV, Fortovase, Invi...

Explain Saquinavir Saquinavir (SQV, Fortovase, Invirase) - When administered as a single protease inhibitor, a soft-gel preparation (Fortovase) with improved bioavailability an

Muncipal water treatment , The modern water treatment plan is often call...

The modern water treatment plan is often called upon to perform wonders with the water fed to it.  The clear, safe , even tasteful water that comes from a faucet may have started a

Define protein-energy malnutrition - nutritional deficiency, Define Protein...

Define Protein-Energy malnutrition - Nutritional Deficiency? Even in normal times, PEM is a problem in many developing countries, most commonly affecting children between

What is the reason for the low red blood cell count, A patient has a low re...

A patient has a low red blood cell count, and microscopic examination reveals an abnormally high proportion of circulating reticulocytes. Upon subsequent examination, the patient i

How can we keep our brain healthy in older age, Is there anything I can do ...

Is there anything I can do to keep my brain healthy in older age? It is increasingly clear that how we live our lives on a daily basis strongly influences how well our brain ag

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd