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Explain the Alkaline Copper Reduction Method?
Since alkaline copper reduction methods are by far the most commonly used methods for blood glucose estimation, let us look at the details of the method. This time honoured method for glucose estimation dates back to 1920 when Folin and Wu devised a method to estimate ‘sugar' in blood by alkaline copper reduction exploiting the reducing property of glucose. Later Nelson and Somogyi modified this method.
Sucrose is a non-reducing Sugar while Maltose and Lactose are reducing due to the presence of free Aldehydic Functional Group in them. In SUgar the Aldehydic Group of both Glucose
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