Explain methodology required for osazone test, Biology

Assignment Help:

Explain Methodology Required for Osazone Test?

Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately.  Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).

Take  slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.


Related Discussions:- Explain methodology required for osazone test

Define functions of magnesium - macro minerals, Define functions of magnesi...

Define functions of magnesium - Macro minerals? Like Ca, Mg too has a role in bone formation. Soft tissue magnesium functions as a cofactor of many enzymes involved in energy m

Zonation in the ocean, Q. Zonation in the ocean? The oceans play a majo...

Q. Zonation in the ocean? The oceans play a major role in determining the climate and sustaining life on earth. Oceans help to redistribute the solar energy, through ocean curr

Radiobiology, Radiobiology : This is the study of effects of radiations on ...

Radiobiology : This is the study of effects of radiations on living organisms. Radiobiology is a branch of science which concerned with the action of ions. Radiobiology is the radi

What is asexual reproduction, What is Asexual Reproduction? Asexual re...

What is Asexual Reproduction? Asexual reproduction occurs in prokaryotes through the process of binary fission. Primitive eukaryotes and plants also reproduce by various t

Show water permeability of the luminal membranes, Healthy Person H takes a ...

Healthy Person H takes a new drug named ANTICAMPCOLLDUCT that blocks the production of cyclic AMP (cAMP) in collecting duct epithelial cells in response to vasopressin binding to V

Pour plate method, what is advantages and disadvantages of the pour plate m...

what is advantages and disadvantages of the pour plate method

Chlamydiosis-pathogenesis, Pathogenesis The mode of intracellular repl...

Pathogenesis The mode of intracellular replication of chlamydial agents is investigated morphologically and cytochemically with both light and electron microscope. It is estab

Taxonomy, Agroup of realated genera are classified as-?

Agroup of realated genera are classified as-?

Arthropod classes characterized to the body division, Q. How are the three ...

Q. How are the three major arthropod classes characterized according to the body division? In arachnids and crustaceans the head is fused with the thorax forming the cephalotho

Where in the cell can ribosomes be found, Where in the cell can ribosomes b...

Where in the cell can ribosomes be found? What is the main biological function of ribosomes? Ans) Ribosomes can be found free in the cytoplasm, adhered to the outer side of the

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd