Explain methodology required for osazone test, Biology

Assignment Help:

Explain Methodology Required for Osazone Test?

Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately.  Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).

Take  slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.


Related Discussions:- Explain methodology required for osazone test

Explain in brief about life style modification, Explain in brief about Life...

Explain in brief about Life style modification Life style modification in simpler words, means  diet control and doing exercises. Diet control and exercise should be planned on

Heredity and variations, HEREDITY 1.         Like begets like i.e; ever...

HEREDITY 1.         Like begets like i.e; every living organism reproduces its own kind. Frog reproduce frogs, rats reproduce rats only. 2.         Heredity includes the cha

How does the cellular immune response take place, Q. How does the cellular ...

Q. How does the cellular immune response take place? The lymphocytes that participate in the cellular immune response are the T lymphocytes and T lymphocytes differentiate into

Define carbohydrate metabolism of manganese, Define Carbohydrate Metabolism...

Define Carbohydrate Metabolism of manganese? Mn is required for carbohydrate metabolism. Enzymes pyruvate carboxylase and phosphoenol pyruvate carboxy kinase involved in glucon

Regulation of ammonia assimilation, Regulation of Ammonia Assimilation ...

Regulation of Ammonia Assimilation Heterotrophic bacteria like Escherichia coli and Klebsiella aerogenes induce the operation of GS-glutamate synthase pathway of ammonia assim

Haematology, differenciate between plasma and serum

differenciate between plasma and serum

Name the organism included in symbiotic nitrogen fixation, Name the organis...

Name the organism included in symbiotic nitrogen fixation. What are the components needed for this pure? Determine their role. Differentiate among inbreeding and heterosis. The

Cholesterol, CHOLESTEROL Other name is parental steroid. It is ...

CHOLESTEROL Other name is parental steroid. It is present all over the body but absent in cerebrospinal fluid. Cholesterol is found exclusively in animal food. It

Explain transport and utilization of vitamin a, Explain Transport and Utili...

Explain Transport and Utilization of Vitamin A? The efficacy of the intestines to facilitate absorption and utilization of retinoid s and carotenoids depends upon the cellular

Orders, order of the phylum protozoa

order of the phylum protozoa

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd