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Explain Methodology Required for Osazone Test?
Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately. Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).
Take slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.
The genetic code is the group of rules by that information encoded within genetic material DNA or mRNA sequences is changed into proteins (amino acid sequences) by living cells. Th
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Congenital Aortic Stenosis : The valve may be unicuspid, bicuspid or tricuspid. Rarely it is a dome shaped diaphragm. Uni commissural aortic stenosis produces significant sy
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