Explain methodology required for osazone test, Biology

Assignment Help:

Explain Methodology Required for Osazone Test?

Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately.  Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).

Take  slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.


Related Discussions:- Explain methodology required for osazone test

Name the food yersinia enterolytica infection isolated from, Normal 0 ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4 Determine the Yersin

Define needs of fluid in postoperative nutritional care, Define Requirement...

Define Requirements of Fluid in Postoperative Nutritional Care Extensive fluid losses may occur through vomiting, haemorrhage, diesis, excudate, fever and sweating after a surg

Respiration in adult amphibians and fishes, Q. Do amphibians have direct de...

Q. Do amphibians have direct development? In amphibians the embryonic development is indirect there is a larval stage. Q. How different are the respiration in adult amphibi

Determine the symptoms of dehydration, Determine the Symptoms of Dehydratio...

Determine the Symptoms of Dehydration? The symptoms of dehydration are: Thirst Loss of appetite Decreased urination Impaired physical performance Nau

Explain the diarrhoeal management strategies, Explain the Diarrhoeal Manage...

Explain the Diarrhoeal Management Strategies? The diarrhoeal management strategies have had a major impact on less than 5 mortality rate. The distribution of ORS packets and ne

Explain the procedures involved in blood doping, Describe the procedures in...

Describe the procedures involved in blood doping. What are the physiological mechanisms behind blood doping that attempt to achieve the desired outcome(s).

What is osseointegration, What is Osseointegration Osseointegration was...

What is Osseointegration Osseointegration was the hallmark of success in implant dentistry in the 1980s. It was believed that an implant was successfully integrated when there

Vitamins requirement in dyslipidemia, Q. Vitamins requirement in dyslipidem...

Q. Vitamins requirement in dyslipidemia? Antioxidants and flavonoids, natural vitamin E, vitamins C and Aare nutrients (vitamins) that scavenge cell-damaging free radicals and

Define gonads and reproductive system, Define Gonads and Reproductive Syste...

Define Gonads and Reproductive System? With aging, there is decrease in pituitary hormone secretion causing decrease in gonadal hormone production, which causes number of chang

State the strengths of brief screening measures, Strengths of Brief Screeni...

Strengths of Brief Screening Measures 1) Inexpensive, rapid, portable 2) Needs less training in administration and interpretation 3) Differentiating between dementia and

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd