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Explain Methodology Required for Osazone Test?
Take about 250-300 mg of phenylhydrazine mixture in the 3 clean labelled 15 ml dry test tubes. Add 5 ml of the glucose, fructose and galactose solution to each, separately. Shake and mix the contents of each tube and heat in a boiling water bath (water in the water bath should be boiling vigorously when you put the tubes and should continue to do so till the tubes are removed) for 30- 45 minutes. Remove the tubes from the water bath and put them in a test tube rack. Allow the tubes to cool slowly (not under the tap).
Take slides, label them as Glu, Gal, Fru, Mal, lac, Suc and with the help of a dropper take out a drop of the yellow precipitate like crystals from each tube and put it on the respective slide. Examine the crystals on each slide microscopically.
Parasitic Protozoan Out of the thousands of species of Protozoa, the majority are free living. However, many species from within the phyla Sarcomastigophora and Ciliophora are
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Name the high-energy complex generated during the conversion of succinyl CoA to succinate in the citric acid cycle GTP or ATP is the high-energy complex generated during th
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Define Procedure for Testing the Presence of Sugar in Milk? 1. Take 0.1 ml of milk sample in a test tube. 2. Add 0.2 ml of resorcinol solution (0.05 gm of resorcinol in 100
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Define Observation for Benedict Test - Carbohydrates? An insoluble reddish brown precipitate of cuprous oxide will be obtained. This is similar to Fehling's test. The reddis
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