Determine reagents for determination of protein content, Biology

Assignment Help:

Determine Reagents for Determination of Protein Content Using Biuret Method?

Collect the following reagents to conduct the experiment:

1. 0.2 N NaOH solution - Dissolve 8 g of NaOH pellets in 1 litre of distilled water.

2. Biuret reagent - Dissolve 90 g of Rochelle salt (sodium potassium tartarate) in 500 ml of 0.2 N sodium hydroxide solution. Add 10 g of copper sulphate and 10 g KI and volume is made to 2 L with 0.2 N alkali.

3. Working standard - Dissolve 200 mg albumin in 100 ml distilled water (2 mg/ml).


Related Discussions:- Determine reagents for determination of protein content

Define the needs for enteral feeding, Define the needs for Enteral Feeding?...

Define the needs for Enteral Feeding? For enteral feeding following need to be considered: - In preterm calorie density to be 0.8 Kcal/ml, - LBW formulas used: protein -

Which technique would use to see if a particular protein, Which technique w...

Which technique would you use to A) see if a particular protein bound to a piece of DNA B) Detect exactly where on a piece of DNA a protein bound.

Deterimne when lysosomic enzymes play a fundamental role, What are some bio...

What are some biological examples in which lysosomic enzymes play a fundamental role? The remodelation of the osseous tissue, the function of acrosomes in sperm cells and the e

Crustacea, Crustacea The Crustacea show a great morphological diversit...

Crustacea The Crustacea show a great morphological diversity of appendages which may be grouped into three categories, cephalic, thoracic and abdominal. Their appendages are c

Explain about the derived proteins, Explain about the Derived Proteins? ...

Explain about the Derived Proteins? These are not naturally taking place proteins and are obtained from simple proteins by the action of enzymes and chemical agents, heat, mech

Define the equilibrium potential for potassium ions, A complete motor neuro...

A complete motor neuron is removed from a frog and placed in a large volume of normal physiological saline.  The neuron is healthy; it has a stable resting voltage of -70 millivolt

Divisions and representing species of the gymnosperms, Q. What are the majo...

Q. What are the major divisions and representing species of the gymnosperms? This group of plants can be separated into conifers (pine, sequoia, cypress), that have flowers cal

Succession - community change, Succession - Community Change The facto...

Succession - Community Change The factors like fire, floods and human interventions affect an ecosystem considerably. They often lead to the depletion or stripping off of orig

Littoral zone, Littoral Zone Plants of the littoral zone Two types ...

Littoral Zone Plants of the littoral zone Two types of plants occur here: Non-rooted phytoplanktons which include all kinds of algae occurring in the limnetic as wel

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd