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Define reagent required and methodology for Barfoed Test?
Reagents
- Solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch
- Barfoed's reagent (copper acetate in glacial acetic acid)
Methodology
Take 5 ml of glucose solution and add 6-10 drops of Barfoed's reagent. Boil for about 1 minute. Observe carefully. Repeat this test taking other carbohydrates.
Packs, pouches, and cassettes are sometimes wet after completing the sterilization process. This is a problem usually associated with the use of paper or paper/ plastic wraps in t
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