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Define reagent required and methodology for Barfoed Test?
Reagents
- Solutions of glucose, fructose, galactose, lactose, maltose, sucrose and starch
- Barfoed's reagent (copper acetate in glacial acetic acid)
Methodology
Take 5 ml of glucose solution and add 6-10 drops of Barfoed's reagent. Boil for about 1 minute. Observe carefully. Repeat this test taking other carbohydrates.
Define Precautions for Determination of Haemoglobin Content in Blood 1. Potassium cyanide is highly toxic and hence drabkin's solution should not be pippeted by mouth. 2. Dr
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