Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Supply of animals in lab, Supply of Animals: Lab animals must be obtained ...

Supply of Animals: Lab animals must be obtained from accredited dealers and by accredited dealers, we mean suppliers in the business of supplying animals for lab use, and not from

Phlum mollusca, about phylum mollsca and its classification

about phylum mollsca and its classification

Define iron response element and iron regulatory protein, Define Iron Respo...

Define Iron Response Element and Iron Regulatory Protein? The Iron Response Element (IRE) and the Iron Regulatory Protein (IRP) play key roles in co-ordinating regulation of ir

Explain the microscope, Explain the Microscope? Microscope is a powerfu...

Explain the Microscope? Microscope is a powerful and crucial basic tool in the field of microbiology. Microbiology, as we have already studied in our theory Course, is the scie

State about bone physiology, State about Bone physiology Bone physiolog...

State about Bone physiology Bone physiology that is most relevant to the study of mechanically mediated bone adaptation and its relevance in implant dentistry. These aspects in

The mirrows reach or grayling zone, The mirrows reach or grayling zone ...

The mirrows reach or grayling zone This zone has a less steep gradient, than the trout beck. The river here still flows swiftly, though the current is not fast and conditions

How is the cerebrum anatomically divided, Q. How is the cerebrum anatomical...

Q. How is the cerebrum anatomically divided? The cerebrum is divided into two cerebral hemispheres, the left and the right. Each hemisphere is made of four cerebral lobes: pari

Uncomplicated q wave mi-coronary artery bypass surgery, Uncomplicated Q ...

Uncomplicated Q Wave MI :  CABG has very little place in cases of uncomplicated Q wave myocardial infarction. However the place of urgent surgery has to be compared to thrombo

Kin selection, While explaining the concept of natural selection, we have s...

While explaining the concept of natural selection, we have stressed the fact that natural selection is synonymous with differential or non-random reproduction. Natural selection ai

Evaluate what is the frequency of carriers in a tank, Zebrafish are raised ...

Zebrafish are raised in fish tanks with one family per tank. The mutation spadetail is a recessive lethal. A stock tank for spadetail was made by crossing two carriers. The fish in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd