Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Scentific american article suggests some improvements, The human skeleton h...

The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like backage and the k

How sequences of events occur to activate protein kinase a, When glucagon b...

When glucagon binds to it's receptor on a liver cell, which one of the following sequences of events occurs to activate protein kinase A? -activation of G protein, activation of

Nucleic acid hybridization, Nucleic acid thermodynamics is the learning of ...

Nucleic acid thermodynamics is the learning of how temperature affects the nucleic acid structure of dsDNA (double-stranded DNA). The melting temperature (Tm) is explained as the t

Define the cooper''s run (12 minute run) method, Define the Cooper's Run (1...

Define the Cooper's Run (12 minute run) Method? To undertake this test, a 400 metre running track is required-marked every 100 m and a stop watch. The test comprises of noticin

Explain gluten-free diet, Explain Gluten-free diet Gluten-free diet : ...

Explain Gluten-free diet Gluten-free diet : It is a diet recommended for the patients with gluten enteropathy. Gluten is present in wheat, rye, barley and oats. Thus, foods co

Is neuron a synapses onto neuron b, Is Neuron A synapses onto Neuron B ...

Is Neuron A synapses onto Neuron B Consider Neuron B in the frog central nervous system whose plasma membrane has a newly discovered ligand-gated ionotropic receptor, named the

Explains mechanisms by which histones interact with dna, Which of the follo...

Which of the following best explains mechanisms by which histones interact with DNA? A. Histone core proteins interact with the main groove of DNA by the helix-turn-helix-turn

Define endurance capacity by standard running tests, Define Endurance capac...

Define Endurance capacity by standard running tests? Endurance capacity, on the other hand, is measured by standard running tests in which time to exhaustion is taken as the en

How do white cells differ from red cells, How do white cells differ from re...

How do white cells differ from red cells (a) In their structure, (b) Their function?   a) White cells have nuclei, red cells do not have nucle

What do you mean by subxiphoid long axis sweep, Q. What do you mean by Subx...

Q. What do you mean by Subxiphoid Long Axis Sweep? This sweep begins with keeping the transducer at subxiphoid region, positioning directly posterior with marker pointing towar

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd