Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Explain acid butt - carbohydrate utilization pattern test, Acid butt - carb...

Acid butt - carbohydrate utilization pattern test ? Acid butt (yellow) and acid slant (yellow) with or without gas production – Glucose along with lactose and/or sucrose, is deg

Explain the bone implant interface, Q. Explain the Bone Implant Interface? ...

Q. Explain the Bone Implant Interface? Three phases have been described in the development of bone implant interface. 1. Stabilization phase Subendosteal and subperiost

Consider the evolution of metabolic pathways, Consider the evolution of met...

Consider the evolution of metabolic pathways. Some biologists believe that these pathways evolved from?

What is gene therapy, What is Gene therapy Gene therapy targets non ger...

What is Gene therapy Gene therapy targets non germ line cells, ie somatic cells, so will not directly affect gene pool. The frequency of the allele for the genetic disease w

Hydrarch - ecology, Hydrarch - Ecology Its various stages are well stu...

Hydrarch - Ecology Its various stages are well studied in ecosystem like ponds, pools and lakes. Since pond is a fresh water ecosystem, the succession in it is also referred t

How water balance take place in human body, How Water Balance Take Place in...

How Water Balance Take Place in Human Body? The amount of fluid in the body is tightly controlled because imbalance can be devastating. In a normal individual, the maintenance

Interventricular septum, It corresponds to the anterior and inferior interv...

It corresponds to the anterior and inferior interventricular grooves. Its right side is convex and bulges to the right ventricle. The septum has a posterior border which separates

Ecology, What is the concept of minimum and maximum way of tolerance

What is the concept of minimum and maximum way of tolerance

Phases of menstrual cycle - reproduction, Phases of Menstrual Cycle - Repro...

Phases of Menstrual Cycle - Reproduction Four phases of the menstrual cycle are usually distinguished: the menstrual, proliferative (follicular), ovulatory and progestational

Imaginal discs, Imaginal Discs In the holometabolous larva, there are ...

Imaginal Discs In the holometabolous larva, there are two cell populations: (1) The larval cells that are used for the larval structures and (2) The imaginal disc and th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd