Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

State the term - migraine stroke, State the term - Migraine Stroke Sinc...

State the term - Migraine Stroke Since the late 1800s, physicians have recognised that migraine attacks may lead to infarcts and permanent neurological deficits. Such migraine

Pathogenesis, The interactions between the human host and selected microorg...

The interactions between the human host and selected microorganisms that culminate in IE involve the vascular endothelium, hemostatic mechanisms, the host immune system, gross anat

What do you mean by wound closure, Q. What do you mean by Wound closure? ...

Q. What do you mean by Wound closure? Generally the incisions designed for minimal exposure such as the H - shaped incision, do not require suturing, as the abutment and the tr

All or None Response, All or None Response A very weak stimulus does n...

All or None Response A very weak stimulus does not result in an action potential. The stimulus must have a certain strength to evoke an excitation. This is the threshold value

Difference between carriers of hiv and aids patients, What is the differenc...

What is the difference between carriers of HIV and AIDS patients? A person can be a carrier of the HIV without necessarily being affected by the immunodeficiency syndrome at t

Difference between hemoglobin and oxyhemoglobin, Q. How different are hemog...

Q. How different are hemoglobin and oxyhemoglobin? Where is it expected to find a higher concentration of oxyhemoglobin, in peripheral tissues or in the lungs? Oxygen-bou

Secondary prevention- preventive strategies for food allergy, Define Second...

Define Secondary prevention- preventive strategies for food allergy? Secondary Prevention: Attempts to inhibit expression of the disease despite sensitization. These are used f

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd