Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

#Types of Blastula, Give a detailed description of stereoblastula, please ?...

Give a detailed description of stereoblastula, please ?#

Explain adverse effects of enfuvirtide, Adverse effects of Enfuvirtide ...

Adverse effects of Enfuvirtide Almost all patients make local injection site reactions to enfuvirtide, usually having of mild or moderate pain, erythema, induration, nodules an

Define locust bean gum, Locust Bean Gum Locust bean gum (also called Ca...

Locust Bean Gum Locust bean gum (also called Carob bean gum) is extracted from the seed (kernels) of the carob tree (Ceratonia siliqua). Structurally, locust bean gum is compos

What are the cell kinds that form the phloem, What are the cell kinds that ...

What are the cell kinds that form the phloem? What are the major features of those cells? The major cells that form the phloem are the sieve elements and the companion cells an

Explain alterations occurring in fruits and vegetables, Alterations occurri...

Alterations occurring in fruits and vegetables You may already be aware that fruits and vegetables vary greatly in their chemical composition. Though, some generalizations are

How linnaeus classify the plant kingdom, Q. How Linnaeus classify the plant...

Q. How Linnaeus classify the plant kingdom? Linnaeus classified the plant kingdom into 24 classes in his famous work 'Genera Plantarum' (1737) and 'Species Plantarum' (1753). I

Short-day plants (sdp) - plant responses to light-dark cycle, Short-Day Pla...

Short-Day Plants (SDP) - Plant Responses to Light-Dark Cycles These plants only flower or flower more profusely and rapidly when given less than a certain (critical) number of

Signify the term - opercular gill, Signify the term - opercular gill. T...

Signify the term - opercular gill. The gill found in bony fish. Operculum is the outer covering of the gill. When it's drawn away from the body, it pulls water inside the bucca

Hemorrhagic enteritis, H e morrhagic enteritis in Turkeys The viral e...

H e morrhagic enteritis in Turkeys The viral etiology in turkey poults showing sudden death was first described in1930 and thereafter reported in most turkey-producing areas.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd