Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Comparison of cabg and angioplasty, Comparison of CABG and Angioplasty : ...

Comparison of CABG and Angioplasty :  Results of six major randomized clinical trials of multi vessel angioplasty Vs CABG are now available. The main conclusions are: Patient

Which are the cell organelles that participate in cell, Q. Which are the ce...

Q. Which are the cell organelles that participate in cell division and in the formation of cillia and flagella of some eukaryotic cells? The organelles that participate in the

Function of the cornified layer of the skin, What are the main functions of...

What are the main functions of (a) the basal (Malpighian) layer, (b) the cornified layer of the skin?   (a) The basal (Malpighian) layer makes new skin cells and the pigment

Procedures for diagnosis - nucleic acid hybridization, Nucleic acid hybridi...

Nucleic acid hybridization: This is a process by which a suitably labeled DNA or RNA from a known infectious agent is used to probe its complementary sequence unique to t

Can you define p-waves, Q. Can you define P-Waves? Changes in P-wave mo...

Q. Can you define P-Waves? Changes in P-wave morphology have been well described in resting tracings and are very useful in identifying right and left sided hemodynamic alterat

Explain nutrient needs and recommended dietary allowances, Explain Nutrient...

Explain Nutrient needs and Recommended Dietary Allowances? For infants and preschoolers, satisfactory growth is a sensitive criterion of whether needs are met. 50 th percentil

In which organ red blood cells are stored, Surplus red blood cells, require...

Surplus red blood cells, required to meet an emergency, are generally stored in what organ of the human body? Is it the: a) Liver b) Spleen c) pancreas d) Kidneys

What is food pyramid, Food Pyramid The diabetes food pyramid divides fo...

Food Pyramid The diabetes food pyramid divides food into six groups. These groups vary with each other on the basis of size occupied in the pyramid. The largest i.e. grains, br

Describe airway and respiratory support in heart disease, Describe Airway a...

Describe Airway and Respiratory Support in the Newborn ? Like in any other emergency situation a stable airway needs to be established first. Newborns with severe respiratory d

What is the heterophagic intracellular digestion, Q. What is the heterophag...

Q. What is the heterophagic intracellular digestion and How is this process accomplished? Heterophagic intracellular digestion is the breaking into smaller substances of extern

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd