Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Caryopsis - development of fruit, Caryopsis - Development of Fruit In ...

Caryopsis - Development of Fruit In cereals each carpel has one ovule and therefore the mature fruit has, just one seed. During maturation, very little or no cell divisions ar

Give examples of diseases caused by bacteria, Give three examples of diseas...

Give three examples of diseases caused by bacteria, Diseases caused by bacteria contain tonsillitis, blood poisoning, scarlet fever, tuberculosis, typhoid, diphtheria, food poi

What is stem cell therapy, What is Stem cell therapy Most defects / dis...

What is Stem cell therapy Most defects / diseases treated by stem cell therapy are not genetic in origin so the therapy will have no impact on gene pool in terms of change in f

Explain about the artificial sweeteners, Explain about the Artificial Sweet...

Explain about the Artificial Sweeteners? As a group, artificial sweeteners are classed as "non-nutritive". Thus, they provide sweet sensation to the tastebuds, with no increasi

Knotted network of capillaries, A knotted network of capillaries within a n...

A knotted network of capillaries within a nephron, the glomerulus, receives arterial blood and is porous enough to allow much of the fluid portion of the blood to flow out of it al

Nutrition project, what websites can i use to modify my client''s meals?

what websites can i use to modify my client''s meals?

How cleaning agents works, Q. How Cleaning agents works? Food particles...

Q. How Cleaning agents works? Food particles and other debris provide good nutrients for the nlicroorganisms to grow. In fact, the food particles protect microorganisms d

What is the possible dna sequence, Insulin was the first protein to be sequ...

Insulin was the first protein to be sequenced biochemically. Assuming that there were no introns involved in the process, what are the possible DNA sequences that produced the last

Explain the physical methods to control microorganisms, Explain the Physica...

Explain the Physical Methods to Control Microorganisms? The physical methods to control microorganisms involve heat, filtration or radiations. Figure illustrates these methods.

What is the magnitude n, Three point charges are located at the corners of ...

Three point charges are located at the corners of an equilateral triangle. Find the magnitude and direction of the net electric force on the 1.60 uC charge. (Let A = 1.60 uC, B= 6.

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd