Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Benefits of computerization, Benefits of Computerization Computerizati...

Benefits of Computerization Computerization were the following benefits in nursing: Improved professional care  Better accuracy (by decreasing human error)  Effect

Microbiology and parasitology, Describe the methods that are used for the i...

Describe the methods that are used for the isolation and identification of bacteria.(20 marks)

What do you mean by subxiphoid long axis sweep, Q. What do you mean by Subx...

Q. What do you mean by Subxiphoid Long Axis Sweep? This sweep begins with keeping the transducer at subxiphoid region, positioning directly posterior with marker pointing towar

Evolution the eukaryotic cell or the prokaryotic cell, Which type of cell c...

Which type of cell came first in evolution the eukaryotic cell or the prokaryotic cell? Ans) This is an interesting problem of biological evolution. The most accepted hypothesis

Principal sources of excessive nitrate and phosphate, What are the principa...

What are the principal sources of excessive nitrate and phosphate in rivers and lakes? Excessive nitrates and phosphates come majorly from sewage effluents and intensive agric

Karyotype, KA R YOTYPE External morphology of Chromosomes specific...

KA R YOTYPE External morphology of Chromosomes specific for each species of living organisms. Karyotype can be studied in metaphase of mitosis. Karyotype includes th

Protozoa, Locomotion,nutrition and reproduction in ptotozoa

Locomotion,nutrition and reproduction in ptotozoa

Calculate concentration of solution from standard curve, Calculate the Conc...

Calculate the Concentration of Unknown Solution from Standard Curve? i) First take different volume of standard solution (say 0.2, 0.4, 0.6, 0.8, 1.0 ml) and then calculate the

Reducing cost of accident, Reducing Cost of Accident One clear way to ...

Reducing Cost of Accident One clear way to control the cost of accident would be to follow all the rules of book for safety. All the methods of safety which were described ear

Temperate shrublands, These are areas where woody shrubs predominate rather...

These are areas where woody shrubs predominate rather then trees. In regions with a Mediterranean type of climate i .e., hot dry summers and cool wet winters, shrubs grow close tog

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd