Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Polyphyletic theory, Please I need short notes on polyphylrtic theory

Please I need short notes on polyphylrtic theory

Estrous cycle - reproduction, Normal 0 false false false ...

Normal 0 false false false EN-IN X-NONE X-NONE MicrosoftInternetExplorer4

Excretory system, what is the excretory organ of a lizard?

what is the excretory organ of a lizard?

Describe a method of diagnosing diabetes mellitus, Q. In ancient Greece the...

Q. In ancient Greece the father of Medicine, Hypocrates, described a method of diagnosing diabetes mellitus by tasting the patient's urine. What is the physiological explanation fo

Chromosome jumping, Chromosome jumping is the technique whereby one begins...

Chromosome jumping is the technique whereby one begins with a piece of DNA from one area of a chromosome, and obtains clones from nearby areas without cloning everything in betwee

What is the primary energy source for life on earth, What is the primary en...

What is the primary energy source for life on earth? The primary energy resource for life on earth is the sun. The sun plays the vital role of keeping the planet warmed and it

Neontology, Neontology : It is the study of recently formed organisms. Neon...

Neontology : It is the study of recently formed organisms. Neontology science is the part of biology that is in contrast to paleontology. Neontology science deals with now living o

Define phospholipids, a) Lipoproteins are large molecular compounds that a...

a) Lipoproteins are large molecular compounds that are essential to the transport of cholesterol and triglycerides (TGs) within the blood. They contain a lipid core composed of TG

Pathophysiology and medical mangement of acute renal failure, Pathophysiolo...

Pathophysiology of Acute Renal Failure It is usually reversible but the deviation of physiologic function can be extreme and mortality in pediatric age group remains high. Th

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd