Define isotope dilution method, Biology

Assignment Help:

Define Isotope Dilution Method?

The measurement of total body water (TBW) is based on the principle of hydrometry. You must be aware that water is the most abundant substance in the body accounting for 67% to 74% of the body's weight, the amount of water contained in cells varies with different types of tissues. Adipose tissues contain less water than do muscle cells, if one assumes that the distribution of water in fat and fat free tissues is consistent among individuals. The fat% can be estimated by measuring volume of water contained in the body.

This method assumes that the distribution and exchange of the isotope by the body are similar to the distribution and exchange of water. The subjects are given to drink deuterium or tritium. Deuterium is a rare, non-radioactive isotope of hydrogen and ritium is a rare, radioactive isotope of hydrogen. After approximately 3 hours of ingestion of isotope, a urine or blood sample is taken. The amount of isotope contained
Sports Nutrition in the sample is inversely proportional to the amount of water in the body, total body water is measured. The concentration of hydrogen isotopes (deuterium or tritium) in biological fluids (saliva, plasma and urine) is measured and used to estimate TBW.

In this method, it is also assured that the hydration of the FFM is constant for all individuals (- 73% of FFM). Because TBW fluctuates widely within and among individuals depending on age, gender, level of obesity, and disease, large errors may result when hydrometry is used with the two-compartment model to derive reference measures of body composition. Thus, there are potential sources of error, it's expensive and again it is not very practical. Moreover, technical expertise is required.


Related Discussions:- Define isotope dilution method

Pregnancy - fungal infections, Pregnancy - Fungal Infections Amphoteric...

Pregnancy - Fungal Infections Amphotericin B is the preferred treatment for deep fungal infections during pregnancy. Fluconazole is teratogenic in animals and a same pattern of

Animal disease model, Animal disease model The second area of transgeni...

Animal disease model The second area of transgenic application in agriculture is to develop lines of animals resistant to some specific damaging diseases. While the genes of in

How is the lac operon regulated by the sigma factor, 1. Many bacterial gene...

1. Many bacterial genes show adaptive regulation of their transcription. a) How is the lac operon regulated by the sigma factor? b) How is the lac operon regulated by lactose

Which chemical substances produced by water photolysis, Q. What are the che...

Q. What are the chemical substances produced by water photolysis and what is the destination of each of those substances? Free electrons, molecular oxygen and hydrogen ions are

Some common air pollutants: oxides of sulphur, Oxides of sulphur:     ...

Oxides of sulphur:          It includes SO 2 , SO 3 . It is a colorless gas having sharp, pungent and suffocating odour. It is soluble in water and forms H 2 SO 4 . Sourc

Equivalence point and end point - nutritional biochemistry, Equivalence po...

Equivalence point and end point - Nutritional  Biochemistry? Titrimetric analysis volumetrically measures the amount of reagent, often called a titrant, required to complete a

Microbiology, control of microorganisms by chemical method.

control of microorganisms by chemical method.

Describe tranposition of great arteries intact ventricular, Describe tranpo...

Describe tranposition of great arteries with intact ventricular septum? Transposition of great arteries with intact ventricular septum (or small VSD): There is usually inadequa

Define the effect of niacin deficiency on nervous system, Define the effect...

Define the effect of niacin deficiency on Nervous System? Delirium is the most common -mental disturbance in acute pellagra. Dementia is more frequently seen in the chronic cas

Show the inflammation triggered by bacterial infection, Q. Which type of de...

Q. Which type of defense cell do bacteria attract and cause to multiply during the inflammation process? What is the name given to the waste material produced by the inflammation t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd