Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
plz answer me how I arrange my topic with its order ?
The American Heart Association (AHA) has recommended the revised Jones Criteria as a guide for ARF diagnosis. The same have been approved by WHO study group for the diagnosis of in
1. Describe how the various molecular mechanisms act to regulate transcription of the genes within the lactose operon of E. coli . 2. Transcription of a eukaryotic protein-codi
Diagnosis of lyme disease In endemic areas, Lyme disease is diagnosed by recognition of erythema migrans. IgG antibodies to B. burgdorferi are usually detectable 4 to 6 weeks a
advantage sand disadvantages of protozoa
Define the Primary Stain and Mordant? (i) Primary Stain - Crystal violet is the primary or first stain, which stains all the cells violet/purple. (ii) Mordant - Gram's iodin
True or false? It would be difficult to assess whether the drug-susceptible or drug-resistant phenotype in a population of Mycobacterium tuberculosis was more fit in an environment
Q. How does self infection by tapeworms occur? The Taeniasis patients may develop the most severe form of the worm infection, cysticercosis, because their feces contain eggs
The Starch Granule - Structure Starch is a polysaccharide, a chain of many glucose molecules. There are two types of glucose chains in starch. One is a simple chain called am
Importance of cellulose About one third of the world's production of purified cellulose is used as the base material for a number of water-soluble derivatives with predesigne
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +1-415-670-9521
Phone: +1-415-670-9521
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd