Already have an account? Get multiple benefits of using own account!
Login in your account..!
Remember me
Don't have an account? Create your account in less than a minutes,
Forgot password? how can I recover my password now!
Enter right registered email to receive password!
Define Free Solution or Moving Boundary Method?
The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.
Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.
Phosphodiesterase Inhibitors and Other Agents Amrinone and Milrinone are prototypes of type III PDE inhibitors. They decrease the breakdown of cyclic AMP in the myocardium and i
What are Polysaccharides? Polymers of simple sugars are known as Polysaccharides. Several polysaccharides, unlike sugars, are insoluble in water. Dietary fibre includes polysac
The man-made sources of air pollution are broadly categorized into three: Point or stationary sources: it includes such sources which pollute at particular. Pollutants from
Enumerate in detail about the Cytoskeleton All eukaryotic cells have distinct shapes, and are also capable of assuming different shapes. 'The internal organelles of a cell are
Medical Management In chronic renal failure there is irreversible renal failure. The goals of medical management are: To promote maximal renal function To ma
Define the Calcium Toxicity? Elevated blood calcium can occur in association with high parathyroid hormone, hyper- or hypothyroid conditions, bone metastasis, vitamin D toxicit
Explain about the Drying - methods of food processing? Drying is the oldest and probably the simplest way of preserving food. It is usually accomplished by the removal of water
Why Diabetics patient should have Vegetables every day Diabetics should have vegetables every day. Fresh vegetables are to be preferred because they have less fats and salt. Da
Determine what is dermal branchiae? External extensions of outer epidermis and peritoneum of the echinoderm body cavity. Both outer epidermis and inner peritoneum are lined wit
Bone Remodeling Bone remodeling differs from the other means of bone structure alteration in that osteoblasts and Osteoclasts do not act independently but are coupled and bone
Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!
whatsapp: +91-977-207-8620
Phone: +91-977-207-8620
Email: [email protected]
All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd