Define free solution or moving boundary method, Biology

Assignment Help:

Define Free Solution or Moving Boundary Method?

The first electrophoresis technique used in the study of protein was free solution or moving boundary method devised by Tselius in 1937. A buffered protein solution occupied the lower part of a quartz U-tube forming a sharp boundary with protein free buffer solution which lay above it in both limbs of the U-tube and with which electrodes were in contact. On application of an electrical potential difference, proteins migrated to the appropriate electrode at varying speeds and the sharp boundary was disturbed. The position of the boundary for each protein could be determined by observing the change in refractive index which occurs at this point.

Separation occurs only at the boundaries, the bulk of the protein remaining homogenous. Using this technique human proteins can be resolved into albumin and three globulin zones α (alpha), β (beta)and γ (gama), in order of decreasing mobility. This is moving boundary or frontal electrophoresis. The technique is still used in research for the measurement of electrophoretic mobility and for the study of protein-protein interaction but is not used in clinical laboratories for routine work since a complex apparatus is needed, the technique is difficult and samples of the order of 0.5 ml of serum are required.


Related Discussions:- Define free solution or moving boundary method

How do white cells differ from red cells, How do white cells differ from re...

How do white cells differ from red cells (a) In their structure, (b) Their function?   a) White cells have nuclei, red cells do not have nucle

Analysis of amino acid sequence on particular peptide, Define Analysis of A...

Define Analysis of Amino Acid sequence on Particular Peptide? The analysis of the relative order or the sequence in which the ammo acids are arranged-along the length of the

A fruit fly has four pairs of chromosomes in its cells, A fruit fly has fou...

A fruit fly has four pairs of chromosomes in its cells. At meiosis, how many dissimilar combinations of maternal and paternal chromosomes are possible in the gametes? In gamet

Sinus venarum, The interior of the right atrium has a rough anterior part, ...

The interior of the right atrium has a rough anterior part, the atrium proper and a smooth part called the sinus venarum. Also it has an appendage called the auricle. All the large

Doppler evaluation for constrictive pericarditis, Q. Doppler evaluation for...

Q. Doppler evaluation for constrictive pericarditis ? 2D Echocardiography and Doppler evaluation can provide valuable clues to the presence of constrictive pericarditis. M-Mo

What are the two techniques of submerged implants, Submerged implants Decis...

Submerged implants Decision has to be made between two techniques. These techniques are: Technique 1: Stage two using a mucoperiosteal flap Technique 2: Stage two done wi

Germ line gene therapy, Germ line gene therapy - This approach either targ...

Germ line gene therapy - This approach either targets to correct the totipotent cells within human conceptus or stimulate the genetic modifications within reproductive cells that

Illustrate the dna fragments using gel electrophoresis, Which of the follow...

Which of the following statements regarding the separation of DNA fragments using gel electrophoresis is false? A. DNA fragments will migrate towards the positively charged ele

List the counselling skills, Q. List the counselling skills? Slowly sta...

Q. List the counselling skills? Slowly start winding up the contact with the client and the family by providing them support, motivating the client to take care of oneself with

Define points to keep in mind while feeding a preschooler, Define Points to...

Define Points to Keep in Mind while feeding a preschooler? 1. Do not force-feed the child. 2. Children naturally eat a variety of foods. Their sensory-specific satiety ensur

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd