Composition - culture medium, Biology

Assignment Help:

Composition - Culture Medium

A standard plant tissue culture medium (Basal medium) contains all the essential macro elements (Carbon, hydrogen, oxygen nitrogen, phosphorus, sulphar, calcium, potassium and magnesium) and iron and microelements(iron, manganese, copper, zinc, boron and molybdenum) but the concentration of their salts in different formulations vary. In addition some vitamins and sucrose (2-3%) are universal constituents of plant tissue culture media. The composition of Ms medium (Murashige and Skoog's) medium found satisfactory for a wide range of monocotyledonous and dicotyledons species.

Besides the nutrients, one or more plant growth regulators (PGR7S) are generally required for supporting good growth of the cultured material. The PGR'S most widely used in tissue culture media are auxins (2, 4-D, IAA, NAA, IBA) and cytokinins (BAP, Kinetin). The PGR7s are particularly important for the growth of Callus tissues and organogenic embryogenic differentiation. Usually the medium is gelled with 0.8% bacteriological agar.


Related Discussions:- Composition - culture medium

What are the main cellular features of fungi, What are the main cellular fe...

What are the main cellular features of fungi? There are unicellular and pluricellular fungi. All fungi are eukaryotes and heterotrophs. Fungi have cells with cell wall made

Discuss refractive and response to wound functions of cornea, Discuss the r...

Discuss the refractive and response to wound functions of cornea. Refractive Function: Cornea, through its interaction with the tear film, forms a smooth refractive functi

Factors causing short-term changes in intra-ocular pressure, What Factors c...

What Factors causing short-term changes in intra-ocular pressure 1) Arterial blood pressure 2) Systemic venous pressure (SVP) 3) Mechanical pressure 4) Plasma osmolari

Determine the categories of Taxonomic breakdown, For convenience, living th...

For convenience, living things are placed into different groups. Taxonomic breakdown of living things comprises the following categories: Family, Class, Genus, Phylum, Order, K

What are diastolic compressive forces, Q. What are Diastolic Compressive Fo...

Q. What are Diastolic Compressive Forces? Coronary perfusion pressure equal to pressure gradient between the coronary arteries and the pressure in LV in diastole. When coronary

Species diversity indices, From an ecological point of view, species richne...

From an ecological point of view, species richness alone has limited value. More meaningful measures use the number of different species in a given area (species richness) as well

Post-operative nursing care of a child with cleft lip, Post-operative Care ...

Post-operative Care   The goals of care during post-operative period include:  Prevent the baby from sucking and crying to avoid tension on suture line.  Give proper p

What is phenotypical proportion, Considering hybridization in a given trait...

Considering hybridization in a given trait like the color of the hair of a mammalian species (white/black) conditioned by a pair of different alleles under complete dominance (blac

What is mitosis, What is mitosis? What is the importance of mitosis? Mi...

What is mitosis? What is the importance of mitosis? Mitosis is the process in which one eukaryotic cell separates into two cells identical to the parent cell (generally identic

Paper points & chemicals removal of gutta percha, Paper points & chemicals ...

Paper points & chemicals removal of gutta percha: -  Flushing action: residual of qutta- percha and sealers chemically solvent by floating pulp chamber with chloroform. -  Chem

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd