Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Classification levels of blood pressure, Table gives the current classifica...

Table gives the current classification of hypertension as recommended by JNC VII. The classification is based on the mean of two or more seated readings on two or more occasions.

Properties of fatty acid, A fatty acid having of a hydrocarbon chain and a ...

A fatty acid having of a hydrocarbon chain and a terminal carboxylic acid group that is shown in the figure.  Most  fatty  acids  are  in  biology  have  an  even  number  of carbo

Integumentary system - nails, NAILS - Cover terminal segment of fing...

NAILS - Cover terminal segment of fingers & toes. Highly vascular dermis under lying the nail is called nail bed . It gives pinkish colour to nail. The small part

Is herbivorism a form of predatism, Is herbivorism a form of predatism? ...

Is herbivorism a form of predatism? Herbivorism is a type of predatism in which first order consumers feed from producers (plants or algae). For instance, birds and fruits, hum

What are the habits of patients- implants, Habits of patients- Implants ...

Habits of patients- Implants Smoking has been shown to predispose to poor bone quality by reduced vascularity. Therefore it is prudent not to accept heavy smokers as implant pa

Cellulose, why can only one kind of cellulose be formed?

why can only one kind of cellulose be formed?

Explain amantadine, Amantadine Treatment with oral amantadine or rimant...

Amantadine Treatment with oral amantadine or rimantadine begun within 48 hours after the onset of illness decreases the duration of fever and symptoms by about 1 day. Whether t

Define some precautions for estimation of amount of bacteria, Define some P...

Define some Precautions for Estimation of the Amount of Bacteria? 1. Dilution should be made carefully. 2. Use fresh sterile pipette for making each dilution. 3. Aseptic

Desscribe formation of sperm cells from germ cells, Q. Indicating the name ...

Q. Indicating the name and respective ploidy of each involved cell how can the formation of sperm cells from germ cells be described? The formation of spermatogenesis or sperm

Explain the role of pancreas in digestion, Role of Pancreas in Digestion ...

Role of Pancreas in Digestion Pancreatic  juices  secreted from  the  pancreas  aid  in  digestion  of  the  food. About 600-800  ml  of  fluid  i.e.  the  pancreatic juice  is

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd