Coagulation - blood collection , Biology

Assignment Help:

Coagulation - Blood Collection:

Plasma is used to minimize the time needed for coagulation so it is used is medical emergencies.

There are many types of anticoagulants used nowadays for example:

  • Heparin (salt of mucoitin polysulforic acid) is widely used and causes the least interference with the tests. About 20 units of heparin is needed to anticoagulate 1 ml of blood.
  • Ethylenediaminetetraacitic acid (EDTA): this chelating agent is presented as salt with final effective concentration of 1-2 mg/ml of blood. It should not be used for specimens tested for calcium analysis.
  • Sodium fluoride: this is generally considered as a preservative of glucose (it inhibit enzyme system involved in glycolysis); however it has weak anticoagulant activity. Fluoride at a concentration of about 2 mg/ml is the best preservative for glucose. Most specimens are preserved at 25 oC for 24 hr or 4 oC for 48 hr. Urea can not be estimated (in urease based methods) in a sample with fluoride because it inhibit the enzyme urease.
  • Other anticoagulants used are for example sodium citrate, oxalates, and iodoacetate.

          For estimation of bicarbonate in blood, it should be collected under one inch column of liquid paraffin to avoid exposure to the atmosphere, before it is centrifuged and processed. This is to avoid escape of carbon dioxide.

 

 


Related Discussions:- Coagulation - blood collection

Describe the origin of muscle cramps and pains, Q. How can the knowledge ab...

Q. How can the knowledge about fermentation describe the origin of muscle cramps and pains after intense physical exertion? A typical fermentation process due to oxygen scarcit

Determine how many family full-time family doctors, 1.  In the town of Jasp...

1.  In the town of Jasper, CA, there is currently one full-time family doctor. If the town's population grows at a steady rate of 14% per year, how many family full-time family doc

Type specimen of class enteropneusta, Q. Type specimen of class enteropneus...

Q. Type specimen of class enteropneusta? Examine the specimen and note the following features: 1. Balanoglossus has a soft, elongated, worm-like body which has a ciliated surf

Explain the sensory receptors, Sensory Receptors Sensory information re...

Sensory Receptors Sensory information received by these receptors is transmitted through sensory fibres of peripheral nerves to sensory areas at different levels of spinal cord

Explain sedationin details, Explain Sedationin details? Intubation and ...

Explain Sedationin details? Intubation and mechanical ventilation is associated with a significant degree of discomfort. In addition, many of the procedures done routinely in I

Explain the management of renewable resources, Explain the Management of Re...

Explain the Management of Renewable Resources? Since the human population has grown, human impacts on the resources that we use, like fisheries and forests, have continued to g

Define quest of nutrition required for excercise, Define quest of nutrition...

Define quest of nutrition as sports nutrition as a discipline? Although the quest of nutrition as applied to exercise and sports dates back to ancient civilizations and importa

VIRUSES, WHAT IS THE TYPICAL REPRODUCTION CYCLE OF A DNA VIRUS

WHAT IS THE TYPICAL REPRODUCTION CYCLE OF A DNA VIRUS

Determine some methods of blood glucose estimation, Determine some Methods ...

Determine some Methods of Blood Glucose Estimation? The various types of methods for estimation of blood glucose can be grouped under two groups: a) Chemical methods b) E

Water-soluble vitamins for school children and adolescents, Determine Water...

Determine Water-soluble vitamins needs of school children and adolescents? The suggested requirements are given in Table 15.2 (ICMR 1990). Thiamine is computed as 0.5 mg/1000 K

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd