Apparently. The untwisting, unzipping. Rezipping and retwisting occur sequentially and ensure that each newly formed double helix is exactly like other one. The final result is a pair of sister chromatids. Each chromatid attaches to its other copy, or sister, at a point of constriction called centromere. The centromere is a specific DNA sequence of about 220nucleotides and has a specific location on any given chromosome. Bound to each centromere is a disk of protein called a Kinetochore, which eventually is an attachment site for the microtubules of the mitotic spindle.

As the cell cycle moves into the G2 phase, however, the chromosomes begin condensation. During condensation each sister Chromatid becomes coiled super coiled into a very tightly compacted structure, which can now be seen with the light microscope and is called a chromosome. During the G2phase, the cell also begins to assemble the structure that it will later use to move the chromosomes to opposite poles of the cell.