Define limitations for underwater weighing method, Biology

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Define Limitations for underwater weighing method?

This method is expensive, time consuming and usually requires a lot of equipment and space.
The subject needs to be totally submerged underwater and exhale all of the air from the lungs. This can be difficult for the subjects.
Requires considerable subject co-operation given that multiple trials needed to obtain accurate estimate of underwater weight.
Being submerged under water may be difficult and produce anxiety for some.
Density of FFM assumed may vary if bone density is altered as in the case of elderly or certain disease conditions.

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