Automated dna sequencing, Biology

Assignment Help:

Automated DNA sequencing is now common place which is based on the chain termination method but using a fluorescent dye attached to an oligonucleotide primer instead of using radioactive labeling.  A several  fluorescent  dye is attached  to the primer  for every  of the four  sequencing  reactions  but,  follow  incubation,  all four combinations  are electrophoresed    and combined on one gel lane. An laser detection  systems  then  distinguish  the  self  of  every  termination  product  as  it elutes  from  the gel. The sequence in that the several fluorescent products elute from the gel gives the DNA sequence.

 


Related Discussions:- Automated dna sequencing

Applications of oracle clinical in pharmaceutical industries, Question 1: ...

Question 1: Write the applications of Oracle clinical in Pharmaceutical industries Explain what is "Oracle clinical" Explain different application of oracle clinical

Why don''t we see things upside down, Q. Since the visual images are projec...

Q. Since the visual images are projected in an inverted manner on the retina why don't we see things upside down? Since the crystalline lens is a convex spherical lens it forms

Early-recurrence of angina, Early: Recurrence of angina soon after the pa...

Early: Recurrence of angina soon after the patient resumes activities is either due to inadequate 1.evascula1isation or acute graft closure. In the immediate post-operative per

Define the principle behind cyanmethemoglobin method, Define the Principle ...

Define the Principle behind Cyanmethemoglobin Method? The principle behind the cyanmethemoglobin method is as follow: Principle The haemoglobin is treated with a reagent con

Explain prophylaxis - fungal infection, Prophylaxis   High-risk neutrope...

Prophylaxis   High-risk neutropenic patients, such as those undergoing allogeneic and certain autologous stem cell transplants, and those with hematologic malignancy who are exp

Pedigree charts, How do I complete a pedigree chart for genetics?

How do I complete a pedigree chart for genetics?

Quantification tricuspid regurgitation-echo quantification, Quantification ...

Quantification of Tricuspid Regurgitation Echocardiography and right ventricular angiogram can quantify tricuspid regurgitation. Echo Quantification Trivial: Non susta

What is cyclosis, What is cyclosis? Cyclosis is a type of internal cell...

What is cyclosis? Cyclosis is a type of internal cell movement in which an oriented flow of circulating material is formed and maintained in the cytoplasm by the action of micr

What do you understand by taxonomy, Q. What do you understand by taxonomy? ...

Q. What do you understand by taxonomy? Taxonomy is dependent on many sciences and they in turn are equally dependent on it. The activities of a taxonomist are basic to all othe

What are the major theoretical models, Q. What are the major theoretical mo...

Q. What are the major theoretical models that try to explain the formation of the enzyme-substrate complex? There are two major models that explain the formation of the enzyme-

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd