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Sweetzyme Q is a commercial preparation of immobilized glucose isomerase manufactured by Novo Industries of Denmark. The following data were obtained for immobilized enzyme particles of different sizes:
Diameter
50 pm
100 um
190 pm
300 um
650 um
Vcbs
μxylose isomerized/(min)(μL support)
0.285
0.220
0.19
0.17
S2 (M)
1.5
a) When considering mass transfer in this porous sphere scenario, there are several regions to consider. Draw and label these for a spherical particle.
b) Assume that Da <<0.1 and β≈0. What is the internal effectiveness factor for the largest catalyst particle?
c) Estimate the effective diffusivity of the substrate through the catalyst pores of the largest particle.
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The events in an experiment are mutually exclusive if only one can occur at a time.
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