What enzyme activity is required for cellobiose

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Cellobiose Metabolism Investigation

Question 1) Draw the chemical structures of glucose and cellobiose.

Question 2) Glucose is utilized by C. crescentus by the pathway known as Entner-Doudoroff (ED) which an alternate pathway for standard EMP glycolysis pathway. Compare and contrast both pathways identifying specific steps that distinguishes one from the other.

Question 3) Write the complete Caulobacter crescentusmetabolic conversion pathway of cellobiose into pyruvate. Your pathway must include all enzyme names with CC# and the intermediate chemical structures as you see in a typical biochemical pathway.

Question 4) What enzyme activity is required for cellobiose to enter ED pathway of C. crescentus.

Question 5) Describing the biochemical principle, propose an assay to measure the activity of the enzyme you identified in Question 4.

Reference no: EM133774487

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