Problem of potency of blastema cells, Biology

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Problem of Potency of Blastema Cells

The local cells are accountable for regeneration. Now you could ask which of the blastema cells differentiated into which type of limb tissues? Do differentiated cells like muscles, cartilage etc., lose their specialised properties, proliferate and consequently re-differentiate solely in accordance along with their previous differentiated state that is into muscle, cartilage etc respectively? Or, do the dedifferentiated cells truly turns into pluripotent cells capable of forming a variety of differentiated cells. To serch answer scientists performed an experiment where the skeleton was removed from the limb segment of an adult newt. This was-done by extirpating the bone so that the limb was lastingly without a skeleton. Then the boneless segment was amputated. It was observed that the limb that regenerated also had its skeletal component. This clearly pointed out that skeletal elements can differentiate from a blastema which received no contribution from preexisting skeleton. Thus tissues, in particular skeletal ones, do not require to be formed by cells from their particular tissue counterparts in the stump.

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Figure: Stump counterparts not - needed for regeneration of the distal parts of the limb skeleton. a: forelimb skeleton of a newt. b: the humerus is excised and then the limb is amputated through the upper arm. c: regeneration of distal parts, including skeleton, proceeds from the cut surface distally.


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