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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
Q. Use of Examination Gloves? Examination Gloves - latex, vinyl, nitrile, neoprene Gloves are worn whenever contact with blood, saliva, mucous membranes or blood/saliva-cont
Describe what would happen if people lost all of their mitochondria and explain why it would happen.
binomial nomenclature
The trp (tryptophan) operon which is shown in the figure will have five structural genes encoding enzymes for tryptophan biosynthesis with an upstream Ptrp (trp promoter)
Ask queAffinities of ctenophora with platyhelminthesstion #Minimum 100 words accepted#
Selection of healing abutment It is done depending upon the tissue thickness. Tissue thickness can be measured similar to ridge mapping or sounding procedure, wherein after an
SKELETA L TISSUES - These connective tissues form the endoskeleton of the vertebrates. These support the body, protect the various organs and help in locomotion. Skel
What benefits can commensalism offer to a species? Commensalism may include obtainment of food (for instance, the innocuous bacteria of the human gut), shelter or support (epip
State the term - Neuroanatomists Broadmann Neuroanatomists Broadmann used a combination of staining and microscopy to map the cytoarcitecture (cell structure\type) of human ce
Write in brief about HbA1c. HbA1c is also the primary glycemic (sugar) target in the management of a diabetic patient and should be performed at repeated intervals throughout t
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