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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
Explain the role of solutions? Solutions: A solution consists of a liquid solvent plus substances dissolved in it, called solutes. Solutes may be ions, atoms, or small molec
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What is Chroma in soil Chroma is the relative purity or strength of the spectral colour and its numerical value extend from (/0) for neutral colours to (/8) for the strongest e
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Define respiration in animals
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Immediate Post-operative Care The patient is accompanied to the ICU by a surgeon, anesthetist and the nurse who assisted for the surgery with portable ventilator and ECG
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