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During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?
A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme.
B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).
C. DNA polymerase is not added to the ligation mixture.
D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.
Q. Explain Disorders of the gastrointestinal tract? The diseases and disorders of the gastrointestinal (GI) tract. Have you ever suffered from abnormal symptoms of the gastroin
You observe the following numbers of individuals of each genotype in a population: AA 700 Aa 200 aa 100 a.) what are the observed genotype frequencies in this populaiton?
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In the axon of a nerve cell, A. the voltage-dependent conductance of the voltage-gated potassium channel has a faster time course than the voltage-dependent conductance of the
Contrast sense Sense of contrast is the ability to perceive slight changes in luminance between regions which are not separated by definite borders. This is as important as to
What is the central principle of molecular biology?
explain the relationship between concentration gradient and active and passive transport
WHAT ARE THE CHARACTERISTICS OF POPULATION IN ANIMAL
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A dialogue and cartoon between haemoglobin and chlorophyll
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