During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?

A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme. 

B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).

C. DNA polymerase is not added to the ligation mixture.

D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.

 

Posted Date: 8/24/2013 3:07:28 AM | Location : United States

Ask an Expert

Related Discussions:- Method of cloning dna fragments into plasmids, Assignment Help, Ask Question on Method of cloning dna fragments into plasmids, Get Answer, Expert's Help, Method of cloning dna fragments into plasmids Discussions

Write discussion on Method of cloning dna fragments into plasmids
Your posts are moderated

Write your message here..

Related Questions

Diagnosis and treatment of myocarditis, Diagnosis X-ray chest show... Diagnosis X-ray chest shows cardiomegaly.  ECG shows conduction defect, low voltage QRS, atrial and ventricular disrhythrnias.  Echocardiogram - decreased LV cont

How does the swim bladder of fishes work, Q. How does the swim bladder of f... Q. How does the swim bladder of fishes work allowing fishes to control their depth under water? From Hydrostatics it is known that an object does not sink if its density is les

What is environmental resistance, The Environmental resistance is the actio... The Environmental resistance is the action of limiting biotic and abiotic factors that disallow the growth of a population as it would grow according to its biotic potential. In fa

Initiation of development - development biology, Initiation of Development ... Initiation of Development - Development Biology We know that the various events collectively called the activation programme. These events are directly related to the formatio

Explain the gram staining technique, Explain the Gram Staining technique? ... Explain the Gram Staining technique? Gram staining is a differential staining procedure which divides bacteria into two groups: Gram positive and Gram negative, based on staini

Explain cell surface membrane, Diagram of a cell surface membrane.  ... Diagram of a cell surface membrane.  State the width of a cell surface membrane. (i) On which side of the membrane, shown in Figure, X or Y, is the cytoplasm of the cell?

How does caffeine effect plant growth, How does caffeine effect plant growt... How does caffeine effect plant growth? Minerals like potassium are often found alongside caffeine when it happens in plant sources like coffee beans, and that could help the pl

What best describes the amoebas division, What best describes the amoebas d... What best describes the amoebas division?  (1) It is a Mode of asexual account in which a one parent is involved. The amoeba cell, which is unicellular, separates into two daug

What is nucleotides, If 15% of the nucleotides in a DNA molecule contain gu... If 15% of the nucleotides in a DNA molecule contain guanine, what percentage of the nucleotides having each of the other three bases? Describe your reasoning. As guanine and cy

Planeria, differnce between protonephridia and metanephridia differnce between protonephridia and metanephridia