Method of cloning dna fragments into plasmids, Biology

During our discussion of replication origins we took a step-by-step look at the method of cloning DNA fragments into plasmids. Based on your knowledge of this discussion which of the experimental manipulations would lead to the failure to insert a fragment of genomic DNA into the plasmid vector?

A. Digest plasmid DNA with the BamH1 restriction enzyme and genomic DNA with the EcoR1 restriction enzyme. 

B. Add bacteria that have been transformed with a plasmid having the kanamycin (antibiotic) resistance gene to an agar plate that having chloramphenicol (antibiotic).

C. DNA polymerase is not added to the ligation mixture.

D. Add bacteria that have been transformed with plasmid DNA to a plate that lacks an antibiotic.

 

Posted Date: 8/24/2013 3:07:28 AM | Location : United States







Related Discussions:- Method of cloning dna fragments into plasmids, Assignment Help, Ask Question on Method of cloning dna fragments into plasmids, Get Answer, Expert's Help, Method of cloning dna fragments into plasmids Discussions

Write discussion on Method of cloning dna fragments into plasmids
Your posts are moderated
Related Questions
Define the Osazone Test or Phenylhydrazine Reaction? Compounds with -CO-CHOH group form crystalline osazones with phenyl hydrazine. These osazones have characteristic shapes an

Some observations seen in this fever are: 1.  Massive loss of  lean body mass or muscle due to tissue breakdown (250-500 g muscle tissue is lost/day) leading to excessive nitro

The reported occurrence of ICMAs is 1.2 per cent to 5 per cent of cases. Streptococci and S.aureus account for 50 per cent and 10 per cent of cases, respectively, and are seen with

Match each item in Column A with the corresponding item in Column B regarding infectious diseases Column A Column B a. Pulmonary tuberculosis i. Enteric infectious protozoan diseas


how does amoeba reproduce (the whole process)

Define hypertension, discuss its causes and explain its effects on the body. What role, if any, does medical imaging play in hypertensive patients? Explain and give examples.


Define Applications of Isolated Soybean Proteins in Meat products? The major application of ISP in connection with meat and related products is based on the use of texturized I

What is the acrosome of the sperm cell? How is it formed? The acrosome is a structure that has a great number of digestive enzymes, it is located in the anterior end of the spe