How tyndallisation used for sterilization, Biology

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Q. How Tyndallisation used for sterilization?

John Tyndall devised a process of sterilisation by steaming for a few minutes at 100^oC on 3-4 successive operations separated by 24 hour intervals at room temperatures. The principle is that the first exposure kills all vegetative bacteria; spores will germinate and be killed on subsequent occasions.

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