Explain the Plating Method - Pure Culture Techniques?

Plating method is one of the commonly employed methods for getting a pure culture.  The procedure involves the separation and immobilization of individual organisms on or within the nutrient agar medium. Each viable cell then multiplies and produces an isolated colony - a visible mass of microbial cells on solid surface, which is obtained by multiplication of a single organism. These colonies can then be transferred readily to nutrient broth or nutrient agar slant to get a pure culture.

There are different methods of plating, which are used for getting a pure culture. These include -

1. Streak Plate Method,

2. Spread Plate Method, and

3. Pour Plate Method.

Posted Date: 6/6/2013 6:43:34 AM | Location : United States

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