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Cosmid: The type of artificially constructed vector taken in use for cloning the 35-45 kb of DNA. These are plasmids carrying the phage l cos site (which permits packaging into l capsids), an origin of the replication and an antibiotic resistance gene. The plasmid of 40 kb is quite difficult to put into bacteria, but it can replicate once there. The cosmids, however, have the cos site, and hence can be packaged into l phage heads (the reaction which can be performed in vitro) to permit efficient introduction into the bacteria.
Synthesis of fatty acids requires the movement of citrate out of the mitochondria. This occurs under conditions of high levels of acetyl CoA which is needed to stimulate the activi
What is diffusion? Diffusion is the spreading of substance molecules from a region where the substance is more concentrated to another region where it is less concentrated. For
Q. As a result of the DNA replication two DNA molecules come into existence. Why is it not correct to assert that the two "new" DNA molecules are created? What is the name given to
Explain Protein hydrolysates It is valued for their superior nutritional qualities, including increased bioavailability and reduced antigenecity. Several enzymatic modification
Oral Feeding This is the preferred and most palatable method of feeding for meeting the increased nutritional demands of catabolism, it should be used as long as possible. If
Aerotolerant anaerobes are incapable of aerobic respiration, but can nevertheless grow in the presence of air. Many lactic acid bacteria fall into this category. They can only gene
Sally Subcloner wants to clone a fragment into pUC19 (Map above – From New England Biolabs). Her fragment was a protein coding gene of 2.7 kb, and had XhoI sites on either end (and
Define the term - microfilaments and microtubules The cytoskeleton is, in fact, termed as the "bone and muscle" of the eukaryotic cell, and is composed of a network, consisting
Fishe s - Heart is 2 chambered 1 auricle & 1 ventricle present. Sinus venosus, truncus or conus arteriusus present. Only impure blood come in heart so heart is venous he
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