Explain about the zone electrophoresis, Biology

Explain about the Zone Electrophoresis?

Zone electrophoresis which was introduced about a decade later describes the migration of charged macromolecules in a porous supporting medium such as cellulose paper, cellulose acetate sheets, and agarose gels. Zone electrophoresis differs from the moving boundary electrophoresis in that it produces an electrophoretogram, a display of protein zones, each one sharply separated from neighbouring zones, on the electrophoretic support material.

In the methods, electrophoresis is carried out in a buffered medium at a fixed pH so that the charge on the particles and hence the rate of migration is stabilized. However, complex mixtures of smaller molecular weight ampholytes can be prepared in which the passage of an electric current produces a gradual increase in the pH of the system from anode to cathode as the components become arranged in order of the increasing pH of their isoelectric points. Isoelectric focussing is based on this principle. When a mixture of high molecular weight ampholytes such as proteins is introduced into such a system covering a pH range of their isoelectric points, the molecules migrate towards the anode or cathode until they arrive at the point at which the pH is that of their isoelectric point. At this point, there is no net charge so the molecule then remains stationary. Isoenzymes and genetic variants can be finely resolved by this technique.

Posted Date: 6/15/2013 6:54:49 AM | Location : United States







Related Discussions:- Explain about the zone electrophoresis, Assignment Help, Ask Question on Explain about the zone electrophoresis, Get Answer, Expert's Help, Explain about the zone electrophoresis Discussions

Write discussion on Explain about the zone electrophoresis
Your posts are moderated
Related Questions
Blood from the fetus circulates through the placenta. a) What substances pass (i) from the maternal to the fetal blood, (ii) from the fetal to the maternal blood?

Antibiotic resistance is the result of natural selection mutations, the only source of new genetic information available for bacteria. Rapid reproduction rates variation in bacteri

Nutrient Elements of Plants Let us first find out the chemical composition of plants and see which element nature has selected to support their healthy growth. Only then we wi

Q. What is tuberculosis? How is the disease transmitted? Is there treatment for tuberculosis? The Tuberculosis is a disease caused by the Mycobacterium tuberculosis bacteria wh

Going south of taiga you would reach the temperate regions of the planet. These regions experience moderate temperatures on average that change during four distinct seasons. They h

Explain some Effect of Inadequate nutrition? Inadequate nutrition ranks as one of the major problems of old age. Various factors which may be responsible for the change in one

The allele that causes albinism (p) is recessive to the allele for normal pigmentation (P). A normal woman whose father is an albino marries an albino man whose parents are both no

What is katal katal : the amount of  enzyme that  transforms  1 mol  of substrate into product in one second.

The risk of PVE is greatest during the initial 6 months after valve surgery (particularly during the initial 5 to 6 weeks) and thereafter declines to a lower but persistent risk (0

GRADIEN T THEORY - It was given by T. Boveri & was supported by M.C. Child. Child called it as metabolic axial gradient theory. According to it, the egg has distinct ani