When the chromosomes  are depleted  of histones  they  are seem  to have  a central fibrous 'protein  scaffold'  or nuclear  matrix  to which the DNA is attached  in loops. Therefore, in vivo it seems like in which the next order of packaging have the attachment of the 30 nm fiber to multiple locations on this central protein scaffold in a series of radial loops. Little is known  to how this structure is structured.

figure: Attachment of the 30nm fiber to a central protein scaffold with the loops arranged radially around the scaffold. The diagram on the right represent a representational cross-section bya chromosome.