Titration of inverted honey solution
It involves the titration of the inverted honey solution. Carry out the titration in the following manner.
1) In a conical flask take 5 ml of Fehling A+5 ml of Fehling B.
2) From burette add the inverted honey solution (approx 10 ml) and boil.
3) Add methylene blue indicator do the titration within 3 minutes.
4) Carry out the titration till blue colour changes to red.
5) Calculate total sugar using the formula given next.
Total sugars = 250 X 100 X S /H X M
where,
S = Fehling's factor (as obtained from standardization procedure of CuSO4 undertaken in step 2 of the procedure earlier). Fehling factor (S) is calculated as:
Titre value of standard invert sugar solution × 0.001
H = Volume of inverted honey solution required (burette reading)
M = Mass of honey
Sucrose (% by mass) = (Total sugars - reducing sugar) × sucrose factor where, sucrose factor is 0.95 and reducing sugar value.