biophysics, Biology

Assignment Help:
you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100 micro moler reverse primer, 100 micromolar reverse primer, 200 micromole of each of the nucleotides, a sufficient amount of plymerase and bugges.

Is the amount of product dna in this set up limited by the amount of template dna, primers, or nucleotides?

Related Discussions:- biophysics

Explain about the microbiological study of water, Explain about the Microbi...

Explain about the Microbiological Study of Water? Water is the basic need of life. River, streams and underground water is the prime source for water. However, disposal of indu

Digestive system - mouth, MOUT H - It is as transverse slit. It is ...

MOUT H - It is as transverse slit. It is pseudo type i.e. not open directly into alimentary canal. Mouth is covered by upper & lower movable lips. Movement is due to arb

Effects on ecosystem - air pollutants, Effects on Ecosystem - Air pollutant...

Effects on Ecosystem - Air pollutants The effects of pollutants on ecosystem become visible after a long period of exposure. In highly industrialised countries the vegetation

What are transcription process and the replication processes, Q What are th...

Q What are the differences and similarities between the transcription process and the replication processes? A DNA polynucleotide chain serves up as a template in the replication (

How are living beings divided into two groups, Q. According to the cellular...

Q. According to the cellular organization how are living beings divided into two groups? Cellular beings are divided into two groups, unicellular beings and the prokaryotes who

What is multi-gated equilibrium radionuclide angiography, Q. What is Multi-...

Q. What is Multi-gated equilibrium radionuclide angiography? Multi-gated equilibrium radionuclide angiography (MUGA) is a very accurate method in measuring ejection fraction. R

Agro industrial-early embryonic mortality, Early embryonic mortality E...

Early embryonic mortality Embryo mortality is a serious problem for farmers, practitioners and researchers. The establishment and maintenance of pregnancy is a highly complica

Describe the ideal primary stability, Less than ideal Primary Stability ...

Less than ideal Primary Stability Faulty osteotomy (a bigger osteotomy than required for the selected diameter of implant) can result when excessive force is used to disengage

Explain zoe-cements - materials for root end filling, Explain ZOE-Cements -...

Explain ZOE-Cements - materials for Root End Filling a. Two  most widely accepted are: IRM and Super EBA (S-EBA) b. Both exhibit similar and favorable  c. Clinically and

Explain the functionality of carboxymethyl cellulose, Functionality of CMC ...

Functionality of CMC CMC is soluble in cold water and mainly used for controlling viscosity without gelling. As its viscosity drops during heating, it may be used to improve t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd