biophysics, Biology

Assignment Help:
you are setting up a pcr reaction to amplify a 1000 basepair long dna grament. in your 50 microliter reaction volume you have 100 nanogrM OD RWMPLrw sn QIRH eoufly 50/50 at/gc 100 micro moler reverse primer, 100 micromolar reverse primer, 200 micromole of each of the nucleotides, a sufficient amount of plymerase and bugges.

Is the amount of product dna in this set up limited by the amount of template dna, primers, or nucleotides?

Related Discussions:- biophysics

How will privacy and confidentiality of clinical data, Question 1: What...

Question 1: What are the requirements you need to satisfy for internet usage and remote data capture under 21 CFR parts 11 in a clinical trial? Define 21 CFR parts Me

What do you understand by larval amplification, What do you understand by L...

What do you understand by Larval amplification? A form of asexual reproduction where, during life cycle, a single larval organism can produce large numbers of the next developm

Define estimation of nitrogen and protein content, Estimation of Nitrogen a...

Estimation of Nitrogen and Protein Content Using Micro Kjeldahl's Method? Standardization of H 2 SO 4 Prepare a solution of 0.1 N H 2 SO 4  by diluting 2.8 ml of the con

Explain the history of darwins finches, Explain the history of Darwin's Fin...

Explain the history of Darwin's Finches? The most well known evidence upon which Darwin based his theory was the distribution pattern of the Galapagos finches. The Beagle's voy

Define x-ray chest and coronary angiography, Q. Define X-Ray Chest and Coro...

Q. Define X-Ray Chest and Coronary Angiography? X-Ray Chest It shows cardiomegaly with left ventricle (LV) and left atrium (LA) enlargement. There may be signs of pulmona

Monitoring for infections, Monitoring for infections Many infections ar...

Monitoring for infections Many infections are common in diabetic patient. Most prominent are urinary tract infections. Others are skin problems (carbuncles), ear problems (otit

Response to heavy metal stress, Response to Heavy Metal Stress Severa...

Response to Heavy Metal Stress Several heavy metals emanating from industrial mining and sewage disposal operations contaminate the environment. Cadmium is a common contamina

Thermodynamics, Thermodynamics Thermodynamics  tells us that every tran...

Thermodynamics Thermodynamics  tells us that every transformation of matter chemical  transformation is  accompanied with  energy conversion .For example  when complex  molecul

Define the term -aqueous movement and outflow, Define the term -Aqueous Mov...

Define the term -Aqueous Movement and Outflow There are two movements of the aqueous humour that occur-thermal flow and bulk flow. Thermal flow is, an internal circulation of

Phylum, detail about phylum protozoa?

detail about phylum protozoa?

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd