The oxidative phase generates nadph, Biology

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1)  The oxidative phase  generates NADPH

The oxidative branch of  the pathway  generates NADPH  and pentose-5-phosphate, through the following  reactions:

a)  Glucose-6-phosphate  is  dehydrogenated  to  6-phosphogluconate  via  6- phosphoglucano-lactone by  glucose-6-phosphate dehydrogenase  in  presence of NADP'  and  the cofactors Mg++,  Mn++  or Ca++.  Glucose-6-phosphate dehydrogenase deficiency is an  inherited disease characterized by  haemolytic anaemia if the patient is treated with an  oxidant drug (such as primaquine and sulphonamide) or ingests fava beans.

b) 6-phosphogluconate is oxidized by  6-phosphogluconate dehydrogenase  in  the presence of  coenzyme NADP+ and  cofactors Mg++,  Mn++  or Ca++  to 3-keto 6 phosphogluconate which is decarboxylated  to form ribulose-5 phosphate.

 


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