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Q. Technical Requirements for pulmonary angiography?
Digital subtraction pulmonary angiography with selective pulmonary arterial injections is vastly superior to conventional cut film angiography in all aspects except in resolution.
Contraindications
Absolute: None
Relative
1) Individuals with LBBB may develop complete heart block due to catheter trauma
2) Pulmonary hypertension
3) Anaphylactoid reaction to intravenous contrast
4) Patients on amiodarone
Venous Access
The femoral vein is the preferred venous access site. However, if there is proximal thrombus, then the alternative venous access sites are right or left internal jugular vein, right or left basilic vein in the antecubital fossa.
The trp (tryptophan) operon which is shown in the figure will have five structural genes encoding enzymes for tryptophan biosynthesis with an upstream Ptrp (trp promoter)
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