Single stranded conformation polymorphism analysis, Biology

Assignment Help:

This is a broadly used screening technique that find several genomic mutations in wide number of samples, this technique finds sequence variations because of point mutations or other mutations by electrophoretic mobility difference, as dna that includes a sequence mutation has a calculable mobility difference as compared to wild type dna when exposed to non denaturing conditions.

This technique includes 4 steps

1. Digestion of genomic dna through restriction endonucleases

2. Denaturation in alkaline solution.

3. Electrophoresis on neutral polyacrylamide gel.

4. Transfer it to a nylon membrane.

5. Hybridisation by either dna fragments or rna copies synthesized on each strand like probes.

6. dhplc

Denaturing high pressure liquid chromatography is latest technique and is very useful for screening of large number of sample of mutations, top for germ line mutations, this echnique includes heteroduplex formation among wild type and mutated dna strands to find mutations. Hetro duplex molecules are segregated from homoduplex molecule through ion pair, reverse liquid chromatography on a special matrix along with partial heat denaturation of the dna strands.

 


Related Discussions:- Single stranded conformation polymorphism analysis

Biology, how can i get an demo assignment to solve?

how can i get an demo assignment to solve?

What could have caused the discrepancy, If the class's value did not match ...

If the class's value did not match the expected value of water, let's assume everyone was actually correctly using the devices and there was no "user error." What could have caused

Fragmentation - types of asexual reproduction, Fragmentation - Types of Ase...

Fragmentation - Types of Asexual Reproduction Fragmentation is a phenomenon where parent animal spontaneously (on its own accord) splits into two or more fragments. Each one o

Can you explain aneurysm of aorta, Q. Can you explain Aneurysm of Aorta? ...

Q. Can you explain Aneurysm of Aorta? CXR findings are an enlargement of the involved portion of the aorta. A focal dilatation may simulate a mass or adenopathy. A more general

Cell division, What is telokinesis and how is it different in animal then p...

What is telokinesis and how is it different in animal then plant cells?

What characteristics of fatty acids are false, what characteristics of fatt...

what characteristics of fatty acids are false? (Dissolves in water, used for energy storage, used to build lipids, found in triglycerides, found in steroids)?

Define about the food production, Define about the Food Production? We ...

Define about the Food Production? We know that agriculture comprises the major source of food production. This is very true in a country like ours where the majority of the pop

Explain process to control of breathing, Explain process to Control of Brea...

Explain process to Control of Breathing? The rate of breathing is controlled by the medulla, the segment of brain stem just above the spinal cord. If the brain stem is cut just

Mcnaughton case and law, MCNAUGHTON'S CASE AND LAW: In 1843, McNaughto...

MCNAUGHTON'S CASE AND LAW: In 1843, McNaughton shot and  killed Sir Robert Peel's secretary Edward -Drymmond.  It was  shown  that McNaughton suffered from a number of  delusi

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd