Screening dna libraries, Biology

Assignment Help:

Genomic  libraries  are  screened  by  hybridization with  a  DNA probe which is complementary  to component of the nucleotide  sequence  of the desired gene. The problem should be a DNA restriction fragment or perhaps element of a cDNA clone.  Exchange  approach  is  possible  if  some  of  the  protein  sequence  for  the preferred  gene is known.  Whereas using the genetic code one can then reduced DNA sequence of this category of the gene and synthesize an oligonucleotide with this sequence to act as the DNA probe.

 


Related Discussions:- Screening dna libraries

Explain positive staining technique, Explain Positive Staining Technique? ...

Explain Positive Staining Technique? Here, a stain has a positively charged chromophore that gets attached to the negatively charged outer surface of the microbial cell and thu

What are the major types of lipids, Q. What are the major types of lipids? ...

Q. What are the major types of lipids? The main types of lipids are phospholipids, triglycerides (fats and oils), waxes and steroids.

Explain about the niacin (nicotinic acid) deficiency, Explain about the Nia...

Explain about the Niacin (nicotinic acid) deficiency? Niacin (nicotinic acid) deficiency classically results in pellagva, which is a chronic wasting disease associated with a c

Transmission electron microscopy, Transmission Electron Microscopy The so...

Transmission Electron Microscopy The source of illumination in a TEM is a tungsten filament that emits electrons. A vacuum is created to prevent the collision of electrons with a

Amelioration of mineral deficiency, Amelioration of mineral deficiency ...

Amelioration of mineral deficiency Performance of livestock in the tropics is mainly governed by the quality and quantity of nutrients provided in the diet. In most of the dev

Phylum colentrata, In phylum colenterata if tissues are present in the body...

In phylum colenterata if tissues are present in the body of an organism then why organs are not present as group of tissues makes an organuestion..

Some common air pollutants: particulates, Particulates: These are suspe...

Particulates: These are suspended droplets, solid particles or mixture of two. So all the atmospheric substances that are not gases are called particulates. A number of term

Explain enzymes, Explain Enzymes Enzymes are the proteins that  act  ...

Explain Enzymes Enzymes are the proteins that  act  as  catalysts,  speeding the  rate  at which biochemical reactions proceed but not altering  the direction or nature of th

Planning and implementation of nursing care - anaemia, Planning of Nursing ...

Planning of Nursing Care   The goals of nursing care are:  Administer oral iron supplements and parenteral iron therapy.  Provide nutritional counselling and educate t

What are typical fauna of the tropical forests, What are the typical vegeta...

What are the typical vegetation and the typical fauna of the tropical forests? In the vegetation of a tropical forest broadleaf evergreen trees predominate. On the top of the t

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd