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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Explain primary structure of proteins The primary structure of a protein having of the order in which amino acids are bonded to one another by a peptide bond.
Explain the Food Applications of guar Guar imparts smoothness to ice cream by promoting small ice crystals during the freezing process. Guar gum in the dressing of cot
Differentiate between Total Fertility Rate (TFR) and Replacement Level (RL) Due to uncontrolled excessive hunting the population of tigers in a forest becomes Zero. Discuss the
Br u c e ll osi s It is also called as Bang's disease or contagious abortion as it causes abortion in late pregnancy and infertility. E t iology: It is cause
What are the main proteins that constitute the sarcomere? What is the function of those molecules in the muscle cells? In the sarcomere there are organized myosin and actin blo
what are the negative and positive economic importance of snai
Define Discovery of other Essential Nutrients? Following the identification of the food factors involved in beriberi, pellagra and scurvy, the search was on for other water-sol
Define the Indications for Root-End Resection Apicoectomy a) Persistent..... "Exactly the SAME of Curettage except the biopsy. b) When the apical portion of the root canal s
Explain risk factors and their role in cad ? The concept of risk factors constitutes a major advance for developing strategies to prevent CVD. The Framingham Heat Study played
Glycogen is a multibranched polysaccharide which serves as a part of energy storage in fungi and animals. In humans the glycogen is recognized and stored primarily in the cells of
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