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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Define Herbals - Old books about Plants? During the Middle Ages, following the decline of the Greek and Roman civilisations, little significant botanical progress was made. The
Which of the following is NOT required to determine equilibrium potential of a particular ion? Select one: size of the ion valence of the ion concentration gradient for
Lens Regeneration in Amphibians It is an amazing fact that between all vertebrates only certain amphibians have the unique ability to regenerate lens, iris and retina in adul
General characters of phylum protozoa
Define Instrument for Measurement of Absorbed Radiation? a) Photometer: This is an instrument which it designed to measure the intensity of the beam of light and usually does s
Q. Explain about Diabetes Mellitus? Diabetes Mellitus is a metabolic disorder characterized by chronic hyperglycemia (increased sugar) with disturbances of carbohydrates, fats
Define Historic Example of Evolutionary quantitative geneticists? Evolutionary quantitative geneticists have developed tools to create predictions about the short-range evoluti
what are lipids? how ther absorption takes place in human bodies?
Subphylum Crustacea Crustacea are mainly aquatic arthropods with gills for respiration. Cephalothorax has usually a carapace; appendages biramous but modified for several fun
Phylum Coelentrat a : (9,000 'species) The body is radial and made up of two cell layers. Gastro-Vascular cavity is saclike, tentacles and stinging cells are present. They may
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