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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Define the Dietary and Lifestyle Modifications - Obesity? The management of obesity basically comprises the following three- pronged approach. a) Dietary modifications b)
Nutrient Absorption Except for carbon, oxygen and hydrogen which are provided by CO 2 and water, all the other elements essential for plants are provided by the soil. Hence,
Competence of Larval Tissue or Multiple Responses The responsiveness of different larval tissues to thyroxine is markedly different. The tissues of the tail becomes necrotic
In the previous section we discussed the concept of species in detail and concluded that the concept of biological species and Mayr's definition of species have a wide acceptance a
Explain about the Salting - Methods of Food Processing? Salting: Salting, especially of meat, is an ancient preservation technique. Food is treated with salt or a strong salt s
What is the fact of Molecular Biology on which DNA fingerprint is based? DNA fingerprint, the method of individual identification using DNA, is based on the fact that the DNA o
What are the differences between vertebrates and the other chordates? Vertebrates are dissimilar because they have a spinal column (vertebral column). In these animals the noto
Overheating the bone and exerting too much pressure Minimal temperature elevation during surgical drilling of the bone is a key factor in atraumatic surgical technique. Bone ce
Explain about the Complete proteins? We now know that proteins are composed of amino acids and that proteins differ in their amino acid make-up. Proteins lacking in one or more
Q. What are the substances that are constitute the chromatin? What is the difference between chromosome and chromatin? The chromatin, dispersed in the nucleus, is a set of fila
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