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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Convergent evolution is the development of the similar type of structures in distantly related organisms as a result of adapting to similar environments or strategies of life. For
Neurulation in Amphibians The first step in the neurulation process is the flattening and thickening of dorsal ectoderm to form neural plate. The plate of cells are different
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Define Hypoglycemic effects of Dietary Factor with antinutritional effect? Of the different carbohydrate foods tested for starch digestibility and blood glucose response, those
Whenever a chromosome is replicated the replication machinery passes by the nucleosomes without deleting the histones from the DNA. How this happens is since not fully understood.
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Determine the Structure of Enzymes Since all enzymes are proteins, you will realize that knowledge of protein structure is clearly a pre-requisite to any understanding of enzym
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