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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
what is the symmetry of fungi?
Absence of Passive Fit of the Prosthesis A passive fit of the prosthesis reduces long term stresses in the superstructure, implant components and the bone adjacent to the impla
PROVIDE a specific example for epithelial and connective tissues, how the arrangement of cells helps with tissue functioning?
Ordinary osmosis cannot be used to purify water. If the applied pressure exceeds osmotic pressure then water starts moving from solution side to pure solvent side. This is reverse
Q. How does photosensitivity in annelids, cnidarians and worms differ from insects, cephalopods and vertebrates? In the first mentioned group of animals there are photoreceptor
Two parents who are each known to be carriers of an autosomal recessive allele have four children. None of the children has the recessive condition. What is the probability that
Which Foods allowed in soft diet:- Soups - mildly flavoured - broths and cream soups. Beverages - all Meat - moist, tender meat, fish or chicken, cottage cheese, eggs
Why is the occurrence of eyelids in amphibians in comparison to their absence in fishes and adaptation to terrestrial life? Eyelids associated to lacrimal glands protect and ke
Q. Extractive use of biodiversity? This includes direct use such as harvesting of wild plant species for use as food, fuel, fodder, fibre, shelter or medicine and hunting anima
What is Glucose Intolerance and Diabetes ? Insulin resistance, by perinsulinaemia and glucose intolerance appear to promote atherosclerosis. The prevalence of CAD raises from 2
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