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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Q. In hormonal terms why does menses occur? Menses is the endometrial monthly desquamation that occurs as the progesterone and estrogen levels fall after the regression of the
Starch Gelatinisation Starch is insoluble in cold water but in warm water it swells until its gelatinization temperature begins to lose its structure and leaches out its 8con
Define Amylopectin - carbohydrates? Amylopectin differs from amylose in being highly branched. Short side chains of about 30 glucose units are attached with α (1-6) linkages ap
Q. What are the major parts of ferns? Ferns are constituted by small roots that come downwards from the rhizome stem and often horizontalized. The fronds also arise from the rh
Which is the type of gamete produced by a heterozygous individual? What is the genotypical proportion of these gametes? Heterozygous individuals, for instance, AA, produce two
Acute Bronchitis: Acute bronchitis is inflammation of one or more bronchi and occurs specifically in children younger than four year?. It is usually associated with previ
The main objectives of study of ecology are: (i) To study the distribution of biota local and geographical level. (ii) To study the interrelationship
Q. Strain of Fusarium moniliforme? It was first obtained from a strain of Fusarium moniliforme isolated from southern leaf blight- damaged corn seed as a water soluble toxin.
Explain the Associated procedure of Reinforcement of Patch? Associated Procedures : Coronary artery bypass grafting and repair of mitral valve are also carried out. P
Because of developmental abnormality, the wall of left ventricle of an infant's heart is as thin as that of right ventricle. What would be its explicit effect on circulation of blo
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