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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
what are the methods of seed formation
What are the vascular bundles? How does configuration of the vascular bundles within the stem differentiate monocots from dicots? The Vascular bundles are segments of xylem and
Why is not Mendel's second law always valid for two or more phenotypical traits of an individual? Mendel's second law, or the law of the independent assortment, is valid for g
Disorder of Parathyroid Function: The disorders of parathyroid function may either lead to increased production or decreased production of parathormone. Hypoparathyroid
Mitral valve prolapse (MVP) has emerged as a prominent, predisposing structural cardiac abnormality and in adults accounts for 7 to 30 per cent of NVE in cases not related to drug
distinguishing charecters and classification of protozoa upto phyla
List the parameters under which you will evaluate implant prosthesis The various parameters under which implant prosthesis can be evaluated are: i) Assessment of mobility.
Q.How is it produced and what is the function of gastrin in the digestive process? The existence of food in the stomach stimulates the secretion of gastrin that in its turn tri
Pesticides - Danger to Wildlife Another recently realised danger to wildlife in many parts of the world has come from the development of more effective pesticides. As agricult
Requirements of Dental implants Dental implants require special oral hygiene techniques. The particular technique chosen often depends upon the prosthesis. A removable prosth
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