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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Explain puewabilitation programme in details ? Education, counselling, and behavioural interventions are important elements of cardiac rehabilitation. In this guideline, "educ
FUNCTIONS OF CELL WALL 1. Provides shape to plant cell rigidity to cells. 2. Functions as a barrier to entry of pathogens into the cells. 3. Provides pr
If due to some injury the chordae tendinae of the tricuspid valve of the human heart is partially non-functional, what will be the immediate effect? 1. The flow of blood into t
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Q. How different are the concepts of action potential, resting potential and excitation threshold concerning neurons? Action potential is the maximum positive voltage level ach
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