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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
what are the Obstructive Lesions of Aorto Pulmonary Window? These lesions can be divided into two groups: 1) Ventricular outflow obstructive lesions i.e. Aortic Stenosis (AS) P
Q What are the kinds of cell respiration? There are two types of cell respiration aerobic cell respiration a reaction with participation of molecular oxygen (O2) and anaerobic
Ophthalmic Data are limited on the effectiveness of antimicrobial prophylaxis for ophthalmic surgery, but postoperative endophthalmitis can be devastating. Most ophthalmologist
DERMIS (CORIUM) - Mesodermal in origin. More developed part. Leather is obtained from dermis by tanning. Thinnest in eye lid. Connective tissue present. In it 4 types
Give two cultural practices (not chemical) you could use to control black spot of rose. Based on your current knowledge of the disease triangle, why do you think these cultural pra
How Pregnancy and Lactation affects the bmr? These periods of physiological stress also has an impact on the metabolic rate. Earlier, we studied that REE is highest during the
Autotomy and Regeneration Shedding of body parts in self-defense to avert the attention of the predator-enemy or in any other emergency is a type of autotomy (auto: self, tom
significant role of suicide cells?
You are studying a gene, and its effects on skin cells. You want to find out A) If the protein made encoded by this gene binds to DNA in a particular place B) if the protein binds
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