Retroviral method for gene transfer, Biology

Assignment Help:

Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication.  The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.


Related Discussions:- Retroviral method for gene transfer

What is shunts in congenital heart surgery, What is shunts in Congenital He...

What is shunts in Congenital Heart Surgery? Shunts : Blalock Shunts are connections between systemic arteries such as a subclavian arteiy with its respective pulmonary arteiy

Why is the krebs cycle also called the final common pathway, Why is the Kre...

Why is the Krebs cycle also called the final common pathway of the degradation of organic compounds? The Krebs cycle is known as the final common pathway of the degradation of

What are the male pronucleus and the female pronucleus, Q. What are the mal...

Q. What are the male pronucleus and the female pronucleus? The female pronucleus is the proper haploid nucleus of the egg cell and Male pronucleus is the haploid nucleus of the

Cholesterol, Cholesterol Cholesterol is found in several of the animal ...

Cholesterol Cholesterol is found in several of the animal foods we eat, involving cheese, eggs, and red meat. Just animal cells utilize and synthesize cholesterol. Click the bu

Explain procedure for isolation of pure culture, Explain Procedure for Isol...

Explain Procedure for Isolation of Pure Culture? Now carry out the exercise following the steps enumerated herewith. 1. Label the name of the organism on the bottom of the p

filtration, It is the process of separating colloidal and suspended impuri...

It is the process of separating colloidal and suspended impurities from water by passing it through a porous bed made of fine sand and other proper sized granular materials. Filtra

Mode of nutrition, What is the mode of nutrition of Ascaris?

What is the mode of nutrition of Ascaris?

Describe mitochondria and explain why it would happen, Describe what would ...

Describe what would happen if people lost all of their mitochondria and explain why it would happen.

What are the intermediate hosts of plasmodium, What are the intermediate an...

What are the intermediate and the definitive hosts of the plasmodium? In the life cycle of the plasmodium humans are the middle hosts (where asexual reproduction takes place) a

Meaning of diabetes self management education, Q. Meaning of Diabetes Self ...

Q. Meaning of Diabetes Self Management Education? You have learned that the treatment of diabetes depends upon the cooperation of the patient and also on how actively a patient

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd