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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
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Elaborate the term - Gill slits. Lateral opening in the wall of pharynx which allows water to enter into mouth and exit through the pharynx. This is one of the ancestral charac
Apparently. The untwisting, unzipping. Rezipping and retwisting occur sequentially and ensure that each newly formed double helix is exactly like other one. The final result is a p
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Pheromones Serving Functions Other than Reproduction Besides sexual responses as described above pheromones mediate several other types of behaviour. Alarm pheromones have bee
State the diagnosis of epilepsy A diagnosis of epilepsy is usually confirmed by an EEG. In some epileptics, however, seizures are difficult to demonstrate in this way, except u
Define Trace elements for school children and adolescents? Iodine requirements peak in adolescence. Consumption of iodized salt should be ensured. Adequacy of zinc for growth i
How is the nervous system of nematodes organized? Where are the neural chords located in their body? Roundworms have a ganglial nervous system with an anterior neural ring sho
DIVERSITY OF AMPHIBIANS ASSIGNMET IN BIOLOGY PLWASE HELP ME TO DO THIS ASSIGNMENT AND I CAN GET GOOD MARKS IN THIS ASSIGNMENT SO GIVE ME TNE ASSIFNMENT OF THIS TOPIC DIVERSITY OF A
Explain Vegetable dehydration It reduces the natural water content below the level critical for the growth of microorganisms (12-15%), without being detrimental to important nu
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