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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Do moulds grow better where it is warm or cold? This time put single culture dish in a warm dark place and the other in a cold dark place. Study the dishes after a some days.
which is the molecular basis of inheritennce
What are the possible sources of human embryonic stem cells? Embryonic stem cells can be derived from individual cells of an early embryo, from blood cells in the umbilical co
What is the clinical deficiency presented by hemophilic people? What is the genetic cause of that deficiency? The Hemophilia is a disease characterized by impaired blood clotti
Palaeobiology : This deals with the study of fossils and remains an impressions of the past organisms found in the rock of different ages. Paleobiology is a growing or comparativel
Q. What is the name of the cells capable of making gametes? What is the ploidy of these gamete-forming cells? The cells that form gametes are the germ cells as opposed to the s
Q. What is myoglobin? What is the function of this molecule in the muscle tissue? Myoglobin is a present in muscle pigment and fibers similar to hemoglobin. Myoglobin has a gre
1. In the human, normal pigmentation ( A ) is dominant to albinism ( a ). What results would be expected from the following matings, both genotypically and phenotypically? AA x
In mountain rabbits, the EL-1 gene is located on chromosome 3. Four alleles of this gene have been identified in the population. With respect to EL-1, what is the maximum number of
Define the Ascorbic Acid - Basic Concepts? Ascorbic acid is a water-soluble vitamin, whose structure is shown in Figure. You would have noticed that its structure resembles glu
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