Retroviral method for gene transfer, Biology

Assignment Help:

Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication.  The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.


Related Discussions:- Retroviral method for gene transfer

What is osseointegration, What is Osseointegration Osseointegration was...

What is Osseointegration Osseointegration was the hallmark of success in implant dentistry in the 1980s. It was believed that an implant was successfully integrated when there

Cytological approach, Write an essay on cytological approach in taxonomy.

Write an essay on cytological approach in taxonomy.

What do you understand by pharynx, What do you understand by Pharynx? T...

What do you understand by Pharynx? The region of digestive tract between the mouth and esophagus. In most animals it's muscular and forces food into the digestive tract which l

Excretory system of animals and human urinary system, steps in urine format...

steps in urine formation and the organs/tissues involve in such process?

Name five kingdoms into which living beings are divided, What are the five ...

What are the five kingdoms into which living beings are divided? Which group of living being is out of this classification? The five kingdoms of living beings are the kingdom M

Cellular totipotency, Cellular Totipotency The innumerable cells whic...

Cellular Totipotency The innumerable cells which constitute the body of a higher plant or animal and containing identical genetic material can be traced to a single cell-the

What do you know about cardiothoracic ratio, Q. What do you know about Card...

Q. What do you know about Cardiothoracic ratio? The cardiothoracic ratio (CTR) shown in figure is the transverse cardiac diameter (the horizontal distance between the most righ

Why haploid cell attempted to perform meiosis, What problem would most like...

What problem would most likely to occur if a haploid cell attempted to perform meiosis? a.The cell could not replicate its DNA prior to meiosis. b.The cell could not pair hom

Define insect resistant crops in ecological way, Insect resistant crops (IR...

Insect resistant crops (IRC) Should reduce use of insecticides. This is beneficial to environment because:  -  More beneficial insects around as they are not killed by in

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd