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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Pre-operative and Post-operative Nursing Care of a Child with Cleft Lip: We shall begin with pre-operative nursing care and then focus on post-operative care. As you know that
Differentiate between glycogenesis and glycogenolysis. The synthesis of glycogen in liver and muscle is called glycogenesis. The breakdown of glycogen in the liver (glycoge
most recent classification of parasitic protozoa with its general characters
Briefly describe fluid feeders
Determine requirements of Vitamins at high altitude? It is observed that requirement of vitamins is not different as compared to plains. In humans, exposure to high altitude ha
Regeneration Leading To Asexual Reproduction Regeneration is described as the replacement of the lost parts of the body of an organism. This capacity is present in approximat
Do enzymes act better under acid or basic pH? Most enzymes act in pH among 6 and 8, a range that corresponds to the general acidic level of cells and blood. There are enzymes,
The fragility of erythrocytes The fragility of erythrocytes is impaired in the absence of NADPH generation due to the deficiency of glucose-6-phosphate dehydrogenase thereby ca
Q. Is there any situation in which the DNA is made based on the RNA template? What is the enzyme involved? The process in which the DNA is synthesized having as template a RNA
Descriibe Mechanical Ventilation and Cardiac Performance During spontaneous breathing, the intrathoracic and intrapulmonary pressures are negative, while with mechanical venti
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