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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
In sulfur photosynthetic bacteria what is the molecule that donates hydrogen for photosynthesis? In sulfur photosynthetic bacteria the substance that donates hydrogen is hydrog
Define Drug Effects on Food Intake - Anorexic Effect? Have an anorexic effect, either as a direct effect of the drug on appetite e.g., some antibiotics or because of side-effec
Hypoglycaemia When Driving Hypoglycemia is particularly dangerous if it happens to someone who is driving. People with hypoglycemia may have trouble concentrating or se
Currents in Estuaries Currents in estuaries are caused primarily by tidal action and river flow. Currents are generally confined to channels but velocities up to several knots
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What are the Haversian canals and the Volkmann's canals of the bones? Is the osseous tissue vascularized? The Haversian canals are longitudinal canals show in the osseous tissu
Explain Polio Adults who have not previously been immunized against polio should receive a primary series of inactivated polio vaccine (IPV) if traveling to areas where polio i
Early Pre-operative Care 1. Admission of patient-usual admission procedure to be completed. 2. Assessment of the patient. 3. History 4. Physical examination
Pound cake test In some cases, oil or margarine creaming volume is most accuratelymeasured by preparing a regular pound cake, omitting the chemical leavener andmeasuring the vo
Q. Which is the brain region that receives conscious sensory information? Which is the brain region that triggers the voluntary motor activity? In the brain conscious sensory i
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