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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Functions of Plasma Proteins The proteins of the plasma serve several important functions. They exert osmotic pressure varying from 25 to 30 mm Hg, which regulate
Fibro-osseous Retention V/S Osseointegration Fibro-osseous retention is the tissue to implant contact interposition of the healthy, dense collagenous tissue between the implant
Functionality of cellulose Cellulose has many uses as an anticaking agent, emulsifier, stabilizer, dispersing agent, thickener and gelling agent, but these are generally subsid
Q. Rate Related Bundle Branch Block? The term has often implied the absence of significant coronary or myocardial pathology. It cannot be judged without taking the total clinic
State three conditions in a society which could contribute to a reduction in the birth rate. Birth rate might be decreased by (i) later marriages (as smaller families), (
Give the results of DistaE Dissection ? Results : For acute dissection of different parts of aorta, the mortality is reported as 9 to 33 per cent. In the International Regis
why the small intestine is longer in herbivores as comparison to carnivores animals
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Define Structural Elements - Biological Functions of Proteins? Structural proteins are fibrous proteins. The well known of the fibrous proteins are possibly the keratins, which
Which are the subproducts of the photochemical stage that are essential for the chemical stage of photosynthesis? The chemical stage of photosynthesis depends on NADPH and ATP
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