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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
The human skeleton has evolved from that of four-legged animals. Unfortunately, the adaptation is far from perfect; thus, our upright posture causes problems like back age and the
Explain Potential Health Benefits of Dietary Fibre? A number of experimental studies in animal models, as well as, epidemiological studies have established protective role of d
Q. What is Peaked T-Waves? Exercise induced increase in T-wave amplitude in V2 is quite predictive of anterior wall ischaemia (specificity = 95 percent). It appears that this m
Infusion Pump As you'have learnt earlier, the amount of fluid to be administered to neonate is based on the neonate's weight and physiological status. It is recommended that
Types of Ecosystem Basically ecosystems are of two types : terrestrial and aquatic. If you travel from plains to the mountains in the Himalayas, you notice significant changes
GROWTH RATE IN ANIMALS - Growth rate is different at different periods of life. Growth period in human may be studied in 5 steps - (i) Prenatal - In gestation period fo
A dihybrid cross yields 320 F2 offspring. How many are expected to resemble the homozygous recessive parental?
Measles Adults born after 1956 who have not received 2 doses of live measles vaccine (not the killed vaccine that was commonly used in the 1960s) after their first birthday an
Q. How does the inflammation mechanism work? When some tissue injury other vasoactive and occurs histamine substances (called mediators of inflammation) are released, they caus
VOLVOX CHARACTERS
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