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Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication. The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.
Q. What is Echocardiography? Ans. Echocardiography has become an established and powerful tool for diagnosing presence of CAD and defining its consequence. It can help in
Describe the relationship of different systems in diabetes mellitus Diabetes Mellitus has direct relationship with endocrine system, especially with endocrine part of the panc
What are the positions of actin and myosin molecules in the sarcomere before and during the muscle contraction? Schematically actin filaments attached perpendicularly to both s
What is the natural habitat of E.coli? The E.coli was first recognized in the colon region of large intestine and so it was given the name "coli" (found in colon) they are coli
Determine Some indicators of Malnutrition? A few of the indicators are enumerated below: 1. Indicators related to Government policies a. Nutrition policy b. Nutrition
Ringworms (cutaneous mycoses) Cutaneous mycoses – also called dermatomycoses, occur worldwide and represent the most common fungal diseases in humans. The dermatophytes are th
Explain about the Pasteurization? You must be aware of the various pasteurized products available in the market. The most commonly used product being ‘milk'. Why do we need to
What is Class Gastropoda in animal kingdom? Snails and slugs are referred to as gastropods because of their characteristic body plan. "Gastro" is derived from the Greek word fo
Q. Write the meaning of Metabolism? Metabolism is the set of chemical reactions that occur in living organisms in order to maintain life. These processes allow organisms to gro
Amniocentesis is a way of prenatal testing in which the amniotic fluid is withdrawn from the uterus by the help of a needle. The fluid and the fetal cells it contains are analyzed
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