Retroviral method for gene transfer, Biology

Assignment Help:

Retroviral method: A retrovirus is a virus that carries its genetic material in the form of RNA rather than DNA. In this method retroviruses are used as vectors to transfer genetic material into the host cell, resulting in a chimera, an organism consisting of tissues or parts of diverse genetic constitution. Chimeras are inbred for as many as 20 generations until homozygous (carrying the desired transgene in every cell) transgenic offsprings are born. The method was successfully used in 1974 when a simian virus was inserted into mice embryos, resulting in mice carrying this DNA. In retroviral method, 8-cell stage embryo is removed and infected by virus carrying foreign gene. The retroviral is made defective in such a way that, it can effectively penetrate cells without replication.  The transformed embryo is then implanted back in foster mother. After birth, the matings are carried out to establish transgenic line. Although retrovirus is a good choice as a vector, it can carry only a limited size of the foreign DNA.


Related Discussions:- Retroviral method for gene transfer

What is the procedure of normal saline dressing, What is the Procedure of N...

What is the Procedure of Normal saline dressing 1. First collect the supplies near the patient. Place should be clean with good lighting where it is easy to work. Use a newspap

Define the term- ultrafiltration, Define the term- Ultrafiltration Ultr...

Define the term- Ultrafiltration Ultrafiltration occurs when the flow of a substance across a cell membrane is increased by a hydrostatic pressure. This process was initially b

Write the meaning of polydypsia, Q. Write the meaning of Polydypsia? In...

Q. Write the meaning of Polydypsia? Increased Thirst (Polydypsia): As the patient is losing more water through excessive urination, the patient feels thirstier and tends to tak

Metamorphosis, Metamorphosis The phenomenon of metamorphosis is descri...

Metamorphosis The phenomenon of metamorphosis is described as a process during development which involves a remarkable change in morphology and physiology of the larva, so tha

What is difficult breathing, What is difficult breathing? Difficult Bre...

What is difficult breathing? Difficult Breathing: Tachypnea (respiratory rates consistently more than 451 min), is a characteristic manifestation of heart failure in newborns (

Nervous tissue and its components, NE R V OU S TISSUE The nervous...

NE R V OU S TISSUE The nervous tissue in general develops from the ectoderm of the embryo, but the microgliocytes. It does not have a matrix. The special properti

Camptotheca acuminata, This species is an ornamental tree in China, and has...

This species is an ornamental tree in China, and has yielded the clinically active agents topotecan, irinotecan, and 9 -aminocamptothecin, which are semisynthetically derived from

Lumpy skin disease, L u mp y skin disease The disease, reported from...

L u mp y skin disease The disease, reported from Sudan in 1970 and Egypt in 1988, is caused by a member of the Capripox virus. It affects cattle and is restricted to African

Respiratory chain to produce atp, Decreasing power is available in a cell b...

Decreasing power is available in a cell both as NADPH and NADH but these have quite distinct roles. NADH is oxidized by the respiratory chain to produce ATP by oxidative phosphoryl

Correct regarding a buffer, Select all that are true or correct regarding a...

Select all that are true or correct regarding a buffer: alternately donating and accepting ions a set of chemicals that work together to resist pH change often a weak acid or base

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd