Regents for estimation of vitamin c - colorimetric method, Biology

Assignment Help:

Define Regents for Estimation of Vitamin C - Colorimetric Method?

1. 2, 4 dinitrophenylhydrazine reagent - Dissolve 2 g of dinitrophenyl hydrazine in 100 ml of 9N sulphuric acid and allow to stand overnight. Mix and use.

2. 5% thiourea solution - Dissolve 25 g of thiourea in 500 ml of distilled water.

3. 9N sulphuric acid

4. 85% sulphuric acid

5. 0.6% copper sulphate

6. Colour reagent or Dinitrophenylhydrazine - thiourea - copper sulphate (DTC) reagent  - Mix

7. 5% trichloroacetic acid (TCA)

8. 5% metaphosphoric acid in 10% acetic acid solution - Mix 1000 ml of 5% metaphosphoric acid and 500 ml of 10% acetic acid.

9. Ice for cooling.


Related Discussions:- Regents for estimation of vitamin c - colorimetric method

What is the type of digestive system present in beings, What is the type of...

What is the type of digestive system present in beings of the phylum Arthropoda? Are these animals protostomes or deuterostomes? The digestive tube of arthropods is complete, h

Describe the term - near-far problem, Describe the term - Near-far problem ...

Describe the term - Near-far problem Signals closer to the receiver are received with less attenuation than signals farther away. Given the lack of complete orthogonality, the

What are contraction of sarcomeres of the muscle cells, Q. What are the few...

Q. What are the few examples of movement created by the contraction of sarcomeres of the muscle cells? The handling of a cup of coffee, the peristaltic movements of the bowels,

Organization chart, ORGANIZATION CHART: Organization chart is one of  ...

ORGANIZATION CHART: Organization chart is one of  the visual which helps any visitor to get an understanding of an organization at a glance.  Meaning of Organization Chart

Explain the nutritional and functional role of sulphur, Minerals :-  Sulfur...

Minerals :-  Sulfur Food Source       Widely distributed  Nutritional Functional role Essential nutrient: A constituent of the essential amino acids methionine and

Define the working of cyclomixer, Define the working of Cyclomixer? Thi...

Define the working of Cyclomixer? This is an electrical mixer used for mixing the contents of a test tube. The speed of the mixer can be adjusted to your requirement. This is a

Why ph regulation is important for living beings, Q. Why pH regulation is i...

Q. Why pH regulation is important for living beings? How mineral salts participate in this regulation? The prospective of hydrogen (pH) is a measure of the amount of hydrogen i

Electro magnetic radiation, Electro magnetic radiation Light is one ...

Electro magnetic radiation Light is one form of energy. The light rays consist of tiny particles called photons. The energy carried by photon is called quantum. Light

History of ecology, History of Ecology The roots of ecology lie in Natu...

History of Ecology The roots of ecology lie in Natural History, which is as old as human civilisation itself. As a matter of fact man indulged in ecology in a practical sort of

Explain resistance to infection in nutritional care, Explain Resistance to ...

Explain Resistance to Infection in Nutritional Care? Amino acids help to build the body's defence mechanisms like antibodies, blood cells, hormones and enzymes so as to prevent

Write Your Message!

Captcha
Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd